Production of Grifola frondosa enzymes on solid-state brewery industry wastes

Švagelj, Mirjan; Berovič, Marin; Gregori, Andrej; Pahor, Bojan; Pohleven, Franc

doi:10.1016/j.jbiotec.2007.07.719

Cited by 4 publications

(3 citation statements)

References 2 publications

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“…In order to obtain the nutrients required for growth and fruiting, G. frondosa is assumed to secrete the hydrolytic/oxidative enzymes that catalyze the degradation of the major macromolecular components (cellulose, hemicellulose and lignin) of its growth substrate. However, compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by G. frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle (Orth et al 1993;Svagelj et al 2007;Xing et al 2006;Yoshida et al 2002).…”

Section: Original Papermentioning

confidence: 99%

Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)

Montoya

Orrego

Levin

2011

World J Microbiol Biotechnol

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Cultivation of specialty mushrooms on lignocellulosic wastes represents one of the most economical organic recycling processes. Compared with other cultivated mushrooms, very little is known about the nature of the lignocellulolytic enzymes produced by the edible and medicinal fungus Grifola frondosa, the parameters affecting their production, and enzyme activity profiles during different stages of the developmental cycle. In this work we investigated the enzymes that enable G. frondosa, to colonize and deconstruct two formulations based on industrial lignocellulosic by-products. G. frondosa degraded both substrates (oak-sawdust plus corn bran, and oak/corn bran supplemented with coffee spent-ground) decreasing 67 and 50% of their lignin content, along with 44 and 37% of the polysaccharides (hemicellulose and cellulose) respectively. 35.3% biological efficiency was obtained when using oak sawdust plus corn bran as substrate. Coffee spent-ground addition inhibited mushroom production, decreased growth, xylanase and cellulase activities. However, taking into account that G. frondosa successfully colonized this residue; this substrate formula might be considered for its growth and medicinal polysaccharide production. Although G. frondosa tested positive for Azure B plate degradation, a qualitative assay for lignin-peroxidase, attempts to detect this activity during solid state fermentation were unsuccessful. Enzyme activities peaked during colonization but declined drastically during fruiting body formation. Highest activities achieved were: endoglucanase 12.3, exoglucanase 16.2, β-glucosidase 2.3, endoxylanase 20.3, amylase 0.26, laccase 14.8 and Mn-peroxidase 7.4 U/g dry substrate.

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Section: Original Papermentioning

confidence: 99%

Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)

Montoya

Orrego

Levin

2011

World J Microbiol Biotechnol

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“…In commercial cultivation, sterilized hardwood sawdust of alder and poplar is often used (16). Chung (7) frondosa (18). Polysaccharides are secreted during G. frondosa cultivation in both liquid and solid substrates used for its cultivation (3,24…”

Section: Introductionmentioning

confidence: 99%

Lignin degradation, ligninolytic enzymes activities and exopolysaccharide production by Grifola frondosa strains cultivated on oak sawdust

Mikiashvili¹,

Isikhuemhen²,

Ohimain³

2011

Braz. J. Microbiol.

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Fourteen strains of Grifola frondosa (Dicks.) S. F. Gray, originating from different regions (Asia, Europe and North America) were tested for lignin degradation, ligninolytic enzyme activities, protein accumulation and exopolysaccharide production during 55 days of cultivation on oak sawdust. Lignin degradation varied from 2.6 to7.1 % of dry weight of the oak sawdust substrate among tested strains. The loss of dry matter in all screened fungi varied between 11.7 and 33.0%, and the amount of crude protein in the dry substrate varied between 0.94 to 2.55%. The strain, MBFBL 596, had the highest laccase activity (703.3 U/l), and the maximum peroxidase activity of 22.6 U/l was shown by the strain MBFBL 684. Several tested strains (MBFBL 21, 638 and 662) appeared to be good producers of exopolysaccharides (3.5, 3.5 and 3.2 mg/ml respectively)

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“…More detailed information would be helpful to improve the cultivation conditions for efficient production of the mushroom [2]. Recently, G. frondosa, was cultivated on substrates composed of olive oil press cakes [7], coffee spent ground [8] and spent brewery grains [9], with different supplements. The experiments demonstrated that of G. frondosa mycelium could become an efficient biotechnological process for the production and isolation of fungal b-polysaccharides [2].…”

Section: Introductionmentioning

confidence: 99%

Modeling Grifola frondosa fungal growth during solid‐state fermentation

Montoya¹,

Orrego²,

Levin³

2011

Engineering in Life Sciences

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Grifola frondosa (maitake) is an edible and medicinal mushroom. Considering its increasing popularity, there are limited references for its cultivation. Previous studies demonstrated that carpophore formation is correlated directly with mycelial biomass. The development of a mathematical model for its growth under solid‐state fermentation (SSF) may help to predict the potential of different substrates for maitake production. G. frondosa growth and basidiome development was studied, using oak sawdust and corn bran as substrates. The fungal biomass content was determined by measuring N‐acetyl‐D‐glucosamine (NAGA). It increased steadily for the first 80 days, to a maximum in coincidence with the first fruiting (60.5 μg NAGA/mg dry sample). Two mathematical models were selected to evaluate G. frondosa development, measuring reducing sugars consumption and NAGA synthesis, as an indirect assessment of fungal growth. Both models showed a good fit between predicted and experimental data: logistic model (R2=0.8896), two‐stage model (R2=0.8878), but the logistic model required a minor number of adjustment parameters.

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Production of Grifola frondosa enzymes on solid-state brewery industry wastes

Cited by 4 publications

References 2 publications

Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)

Growth, fruiting and lignocellulolytic enzyme production by the edible mushroom Grifola frondosa (maitake)

Lignin degradation, ligninolytic enzymes activities and exopolysaccharide production by Grifola frondosa strains cultivated on oak sawdust

Modeling Grifola frondosa fungal growth during solid‐state fermentation

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