Production of halogenated compounds byBjerkandera adusta

Spinnler, Henry-Éric; Jong, Ed de; Mauvais, Geneviève; Sémon, Étienne; Quéré, Jean‐Luc Le

doi:10.1007/bf00902719

Cited by 17 publications

(13 citation statements)

References 24 publications

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“…The fungal metabolite 2‐chloro‐1,4‐dimethoxybenzene [17, 18]was a LiP substrate at pH values below 4.0. At pH 5.0, however, no appreciable activity could be detected.…”

Section: Discussionmentioning

confidence: 99%

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Purification and characterization of two lignin peroxidase isozymes produced by Bjerkandera sp. strain BOS55

et al. 1998

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The white-rot fungus Bjerkandera sp. strain BOS55 excretes at least seven lignin peroxidase (LiP) isozymes. Two of these, LiP-2 and LiP-5 (molecular weight 40^42 kDa), were purified to homogeneity. Both isozymes had the same N-terminal amino acid sequence which showed strong homology with LiP isozymes produced by other white-rot fungi. The kinetics of both isozymes were similar. LiP-5 oxidized veratryl alcohol optimally only in the presence of H P O P near pH 3.0 (16.7 U/mg) and LiP-2 did this below pH 2.5 (33.8 U/mg). Also at normal physiological pHs for fungal growth (pH 5.0^6.5) both isozymes were still active. Further characterization of LiP-2 and LiP-5 revealed that the K m for H P O P strongly decreased with increasing pH. As a result of this the catalytic efficiency (TN/K m ) calculated on the basis of the K m for H P O P in the oxidation of veratryl alcohol was constant over wide pH range.z 1998 Federation of European Biochemical Societies.

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“…The fungal metabolite 2‐chloro‐1,4‐dimethoxybenzene [17, 18]was a LiP substrate at pH values below 4.0. At pH 5.0, however, no appreciable activity could be detected.…”

Section: Discussionmentioning

confidence: 99%

“…The most potent peroxidase having the capability to oxidize substrates with a high ionisation potential is lignin peroxidase (LiP) [9]. This enzyme uses small aromatic fungal metabolites such as veratryl alcohol (VA) [15, 22], and 2‐chloro‐1,4‐dimethoxybenzene [17, 18]as a cofactor [19].…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of two lignin peroxidase isozymes produced by Bjerkandera sp. strain BOS55

et al. 1998

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“…2Cl‐14DMB is a 1,4‐dimethoxybenzene derivative, which is naturally produced by white‐rot and litter degrading fungi, such as Bjerkandera adusta and Lepista nuda [16, 17, 23]. In this report, we provide evidence that this natural metabolite can act as a redox mediator in the LiP catalyzed oxidation of Poly R‐478, 4‐MMA and oxalate.…”

Section: Discussionmentioning

confidence: 73%

“…In this report, we demonstrate that the fungal secondary metabolite [16, 17]2‐chloro‐1,4‐dimethoxybenzene (2Cl‐14DMB) can act as a redox mediator in lignin degradation. Our results provide new evidence for the possible role of other fungal metabolites as redox mediators besides VA in lignin degradation.…”

Section: Introductionmentioning

confidence: 90%

2‐Chloro‐1,4‐dimethoxybenzene as a mediator of lignin peroxidase catalyzed oxidations

Teunissen

Field

1998

FEBS Letters

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Poly R-478, 4-methoxymandelic acid and oxalic acid were oxidized by lignin peroxidase (LiP) in the presence of the fungal metabolite 2-chloro-1,4-dimethoxybenzene (2Cl-14DMB), whereas no oxidation occurred in the absence of 2Cl-14DMB. These substrates clearly inhibited the consumption of 2Cl-14DMB and the formation of 2-chloro-1,4-benzoquinone from 2Cl-14DMB by LiP. The results suggest that 2Cl-14DMB can replace the function of veratryl alcohol as a redox mediator in lignin peroxidase catalyzed oxidations.z 1998 Federation of European Biochemical Societies.

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“…Chloroperoxidase-mediated chlorination of humic acids and lignin by fungi improves the bioavailability of these recalcitrant organic compounds and leads to the formation of halogenated aromatic compounds, such as 5-chlorovanillin and 2-chlorosyringaldehyde (Laturnus et al, 2005; Ortiz-Bermúdez et al, 2007). Similarly, wood-rotting fungi produce vast quantities of chloromethane through the activity of SAM-dependent methyl transferases (Spinnler et al, 1994; Watling and Harper, 1998; Gribble, 2004). In particular the genus Phellinus was held responsible for more than 80% of the chloromethane emission from tropical and subtropical forests (Watling and Harper, 1998).…”

Section: Microbial Chlorinationmentioning

confidence: 99%

Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds

et al. 2018

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Organic and inorganic chlorine compounds are formed by a broad range of natural geochemical, photochemical and biological processes. In addition, chlorine compounds are produced in large quantities for industrial, agricultural and pharmaceutical purposes, which has led to widespread environmental pollution. Abiotic transformations and microbial metabolism of inorganic and organic chlorine compounds combined with human activities constitute the chlorine cycle on Earth. Naturally occurring organochlorines compounds are synthesized and transformed by diverse groups of (micro)organisms in the presence or absence of oxygen. In turn, anthropogenic chlorine contaminants may be degraded under natural or stimulated conditions. Here, we review phylogeny, biochemistry and ecology of microorganisms mediating chlorination and dechlorination processes. In addition, the co-occurrence and potential interdependency of catabolic and anabolic transformations of natural and synthetic chlorine compounds are discussed for selected microorganisms and particular ecosystems.

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Production of halogenated compounds byBjerkandera adusta

Cited by 17 publications

References 24 publications

Purification and characterization of two lignin peroxidase isozymes produced by Bjerkandera sp. strain BOS55

Purification and characterization of two lignin peroxidase isozymes produced by Bjerkandera sp. strain BOS55

2‐Chloro‐1,4‐dimethoxybenzene as a mediator of lignin peroxidase catalyzed oxidations

Microbial Synthesis and Transformation of Inorganic and Organic Chlorine Compounds

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