Production of herpes simplex virus fluorescein labelled typing reagents

Typing of Clinical Herpes Simplex Virus Type 1 and Type 2 Isolates with Monoclonal Antibodies

“…There was a highly statistically significant difference between gC-1 and gG-2 used as target proteins (P Ͻ 0.001, chi-square test), indicating that the intratypic variability within these two epitopes differed among the clinical HSV isolates. Since the gC-1 protein was described earlier to be efficiently expressed at the cell surface (12), this protein has been suggested as a suitable target antigen for diagnostic MAbs (17). The domain recognized by the anti-gC-1 MAb was here shown to be an ideal target for the typing of HSV-1 isolates since the epitope was found to be highly conserved among different clinical HSV-1 isolates.…”

“…Despite the fact that the MAb binds to a region with significant homologies to gG-2, no cross-reactivity to HSV-2 isolates was seen, indicating that gC-1 and gG-2 are structurally different within the epitope detected by the B1.C1 MAb. Although type-specific anti-gC-1 MAbs have previously been shown not to cross-react with HSV-2 isolates (7,17), other workers (11) have described such a cross-reactivity, suggesting an intratypic variability for different epitopes within gC-1. A problem discussed earlier in a study comparing commercial kits used for typing of HSV isolates was that MAbs specific for HSV-2 were somewhat cross-reactive with HSV-1 isolates (7).…”

“…Virus culture, followed by antigenic detection of type-specific determinants, is often used as the "gold standard" to discriminate HSV-1 from HSV-2 (1,16). Since several HSV-specific monoclonal antibodies (MAbs) have been shown to identify type-specific epitopes (2,5,11,17), these reagents have been used for type-specific antigen detection. However, the reported sensitivities and specificities for such reagents, including commercially available kits, have been found to vary among the assays in the range between 92 and 100% (4,6,7,17).…”

“…Since several HSV-specific monoclonal antibodies (MAbs) have been shown to identify type-specific epitopes (2,5,11,17), these reagents have been used for type-specific antigen detection. However, the reported sensitivities and specificities for such reagents, including commercially available kits, have been found to vary among the assays in the range between 92 and 100% (4,6,7,17). This discrepancy may be explained by variability in the expression of different HSV proteins or intratypic variability of different epitopes within the same protein among the isolates (11).…”

Typing of Clinical Herpes Simplex Virus Type 1 and Type 2 Isolates with Monoclonal Antibodies

“…Virus culture, followed by antigenic detection of type-specific determinants, is often used as the "gold standard" to discriminate HSV-1 from HSV-2 (1,16). Since several HSV-specific monoclonal antibodies (MAbs) have been shown to identify type-specific epitopes (2,5,11,17), these reagents have been used for type-specific antigen detection. However, the reported sensitivities and specificities for such reagents, including commercially available kits, have been found to vary among the assays in the range between 92 and 100% (4,6,7,17).…”