Production of Hevea brasiliensis Embryos from in vitro Culture of Unpolinated Ovules

Kouassi, Kan Modeste; Koffi, Kouablan Edmond; Gnagne, Y. M.; N'Nan, Oulo; Coulibaly, Y.; Sangaré, Abdourahamane

doi:10.3923/biotech.2008.793.797

Cited by 4 publications

(1 citation statement)

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“…During the in vitro culture of unpollinated ovaries, the sampling period is the main factor affecting the formation of embryos. It has been reported that when the pistil of cotton ( Gossypium L.) is pretreated with pollen from Hibiscus hemp ( Hibiscus cannabinus L.) and the ovary is harvested 5 or 10 d after flowering, Schenk and Hildebrandt and MS media induce ovules, and there is no significant difference in the number of embryogenic ovules or embryo germination frequency (Kouassi et al., 2008). The highest frequency of embryo development was observed in ovules excised from carrot ( Daucus carota L. var.…”

Section: Discussionmentioning

confidence: 99%

In vitro embryo formation and plantlet regeneration from unpollinated ovaries of Lilium L

et al. 2022

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The in vitro culture of unpollinated ovaries is one of the techniques used to induce gynogenesis. To explore the mechanism of lily gynogenesis and obtain haploids to shorten the breeding cycle, the in vitro effects of flower bud sampling time, exogenous plant growth regulators (PGRs), and different concentrations of picloram on embryo formation in unpollinated ovaries of Lilium L. 'Sorbonne' (oriental hybrids) were studied. The results indicated that flower buds ≥8 cm in length were most suitable. The PGRs picloram and 2,4-dichlorophenoxyacetic acid (2,4-D) with 6benzylaminopurine (6-BA) or kinetin were useful in inducing ovule expansion in vitro. However, most ovules did not survive on the media containing 2,4-D, whereas those grown on media containing picloram developed normally. In particular, a lower concentration of picloram (0.5 mg L −1 ) was found to be beneficial to the development of ovules into embryogenic shoots, whereas higher concentrations were more favorable to the development of shoots from the ovary wall. In total, 87 regenerated plants were obtained from unpollinated ovaries of Sorbonne. The chromosome ploidy of 20 of these was determined by counting root tip chromosomes, and eight of the regenerated plants were found to be haploids.

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Section: Discussionmentioning

confidence: 99%