Production of High‐Quality Probiotics by Fermentation

Anandharaj, Marimuthu; Rani, Rizwana Parveen; Swain, Manas R.

doi:10.1002/9781119048961.ch10

Cited by 10 publications

(5 citation statements)

References 94 publications

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“…This is in agreement with the generally accepted fact that pre‐exposure to sublethal levels of a stress factor can lead to a physiological adaptation to subsequent stress (Anandharaj et al . 2017). The induction of adaptive responses can increase the bacterial tolerance to the same stress factor or to others, and prepare strains to withstand more challenging conditions (Lavari et al .…”

Section: Discussionmentioning

confidence: 99%

Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

et al. 2020

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Aims To investigate the influence of different culture media and fermentation conditions on growth performance and viability of three lactic acid bacteria (LAB) strains with potential as silage inoculants, and to optimize spray‐drying in order to enhance survival to dehydration and storage stability. Methods and Results In house‐formulated MRS was a suitable low‐cost culture medium for Lactobacillus plantarum Hv75, Pediococcus acidilactici 3903 and L. buchneri B463. Uncontrolled pH biomass production conferred enhanced stability during storage at 4°C after spray‐drying. The use of whey protein concentrate 35 (WPC)‐maltodextrin (M) as matrix, inlet temperature of 145‐150°C and air flow rate of 601 l h−1 was adequate for the production of dehydrated LAB. According to the desirability function, at this optimized condition, moisture content, yield and solubility were predicted to be 3·96, 73·68 and 90·36% respectively. Those conditions also showed a decrease of 0·855 log CFU per gram after drying, no loss in viability at 4°C for 6 months and 1 log CFU per gram reduction at 25°C. Conclusions Stable and economically feasible dehydrated LAB cultures were obtained using alternative culture media, fermentation under uncontrolled pH and optimizing spray‐drying process conditions through the desirability function method. Significance and Impact of the Study Our results can be utilized for efficient production and commercialization of several dry LAB.

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Section: Discussionmentioning

confidence: 99%

Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

et al. 2020

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“…The absorbance values of the solution, obtained after centrifuging, were measured at both wave lengths of 420 and 560 nm, and β-galactosidase value was calculated in Miller units using the following formula: MU = 1000 × where A1(560) was the absorbance just before assay and A2(560) was the absorbance value of the reaction mixture [9]. (1,2,3) and the presence of bile salt 0.2 ml of cells grown at 37 o C for 48 h were centrifuged, washed twice in phosphate buffer and resuspended in 2.0 ml of normal MRS (pH 6.8 ± 0.2) and MRS with the pH of 1.0, 2.0, and 3.0, respectively (normal MRS medium adjusted to each pH value with HCl) to obtain final counts between 10 7 -10 8 CFU/mL. After 1.5 h and 3 h, 0.1 ml of MRS medium with different pH values was plated out on normal MRS agar plates.…”

Section: β-Galactosidase Assaymentioning

confidence: 99%

“…Among them, Lactobacillus casei attracts a lot of interest due to its valuable properties. L. casei exists in many products used in our life [2]. Despite their long history of safe use, for being used in human, the bacterial strain should be assessed carefully both in vitro and in vivo according to FAO/WHO recommendation [1].…”

Section: Introductionmentioning

confidence: 99%

Improvement of SERS signal measured by portable Raman instrument using random sampling technique

Tiến

Nghĩa²,

Lien³

et al. 2022

Vietnam J. Sci. Technol.

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In recent years, portable Raman spectrometers and commercialized surface-enhanced Raman scattering (SERS) substrates have become increasingly popular. They have turned out to be great tools for both substance detection, identification, and analysis on-site. This work addresses the technique to collect proper Raman spectra using SERS substrates and portable Raman spectrometers. We propose a random sampling technique that gives representative and high-quality spectra with high intensity and good resolution. This technique was tested on a home-built portable Raman spectrometer and SERS substrates based on metal film over nano-sphere (MFON) structure. Experimental results showed that peaks of Raman spectrum collected using random sampling technique are significantly narrower than those of spectra measured in conventional one and prevent samples and SERS substrates from photoinduced degradation. Potentially, this method can promote quantitative SERS and chemical trace analysis using portable Raman spectrometers.

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“…Exposure to acid leads to a proton accumulation inside the cell that may negatively affect the proton motive force (PMF) across the membrane. Besides cell membrane structural damage caused by changes in PMF, acid stress also causes damage to nucleic acids and proteins (Anandharaj et al, 2017). Bifidobacteria are generally considered to have low tolerance to exposure to acidic conditions.…”

Section: Acidmentioning

confidence: 99%

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

Andrade¹,

Almeida²,

Domingos³

et al. 2020

Front. Bioeng. Biotechnol.

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In the last years several human commensals have emerged from the gut microbiota studies as potential probiotics or therapeutic agents. Strains of human gut inhabitants such as Akkermansia, Bacteroides, or Faecalibacterium have shown several interesting bioactivities and are thus currently being considered as food supplements or as live biotherapeutics, as is already the case with other human commensals such as bifidobacteria. The large-scale use of these bacteria will pose many challenges and drawbacks mainly because they are quite sensitive to oxygen and/or very difficult to cultivate. This review highlights the properties of some of the most promising human commensals bacteria and summarizes the most up-to-date knowledge on their potential health effects. A comprehensive outlook on the potential strategies currently employed and/or available to produce, stabilize, and deliver these microorganisms is also presented.

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Production of High‐Quality Probiotics by Fermentation

Cited by 10 publications

References 94 publications

Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

Technological variables influencing the growth and stability of a silage inoculant based on spray‐dried lactic acid bacteria

Improvement of SERS signal measured by portable Raman instrument using random sampling technique

Commensal Obligate Anaerobic Bacteria and Health: Production, Storage, and Delivery Strategies

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