Production of high yields of docosahexaenoic acid byThraustochytrium roseum ATCC 28210

Singh, Ajay; Ward, Owen P.

doi:10.1007/bf01570118

Cited by 58 publications

(24 citation statements)

References 27 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The total fatty acid content of the cell was 466.5 mg/g dry cells, and thus DHA yield was estimated as 4.3 g/L at 26 h in this study. This biomass and DHA yield were much higher than those reported for the genus Thraustochytrium (T. aureum, T. roseum and T. striatum) grown in flask culture under optimal conditions for 5-12 d [24][25][26] , in which the biomass was around 10 g/L and 17.1 g/L and DHA yield was <1.1 g/L and 2.0 g/L in batch culture and fedbatch culture, respectively. Comparative results have been observed in the cultivation of the genus Aurantiochytrium using a fermentor under the same concentration of glucose as in the present study.…”

contrasting

confidence: 59%

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

et al. 2009

View full text Add to dashboard Cite

contrasting

confidence: 59%

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

et al. 2009

View full text Add to dashboard Cite

“…The optimum seawater concentration (50-60%) for biomass production concurs with the findings of Perveen et al (2006). Also, the pH range for growth (pH 5-8) as reported by Singh & Ward (1996) and Perveen et al (2006) was confirmed in this study with these isolates able to grow well at these pH levels. The optimal growth temperature of Schizochytrium sp.…”

Section: Discussionsupporting

confidence: 75%

Untitled

2011

Mycosphere

View full text Add to dashboard Cite

. 2011 -Cultural optimization of thraustochytrids for biomass and fatty acid production. Mycosphere 2(5), 521-531.Two thraustochytrids isolated from fallen mangrove leaves in Subic Bay, Philippines were identified based on their cell morphology, sporogenesis and spore release as: Thraustochytrium sp. SB04; and Schizochytrium sp. SB11. Physiological screening (temperature, seawater concentration, glucose concentration and initial pH) was undertaken on the two isolates for optimization of culture conditions for biomass and total fatty acid production. Both isolates survived and grew well on glucose as their sole source of carbon. Highest biomass production for the two isolates were obtained at lower glucose concentrations (3-5% w/v) in a half-strength natural seawater (50-60% v/v), at near neutral pH (6.0), and incubation temperatures of 20-30°C. Growth curve analysis showed slower growth (1 d lag time) and a shorter stationary phase (less than 1 day) for Thraustochytrium sp. compared to Schizochytrium sp. Additional extracellular enzyme screening showed that both isolates only produced lipase. Analysis of fatty acid methyl ester results showed that Thraustochytrium sp. produced predominantly short chain fatty acids with oleic acid (18:1), a monounsaturated fatty acid (MUFA) making up as much as 71% of the total fatty acids (TFA). Schizochytrium sp., on the other hand, produced high amount of docosahexaenoic acid (DHA) and comprised up to 22.5% of TFA. The large amount of MUFA makes this Thraustochytrium sp. a viable source of oil for biodiesel production. Just as important is the high amount of DHA produced by Schizochytrium sp., a potential source for local large-scale DHA production.

show abstract

“…The DHA productivity by Thraustochytriidae sp. Z105 in flask culture was lower than previous results in fermentors [13,14], but much higher than the most reported results in flask culture [8,12,16,17]. Comparison of DHA productivity, Thraustochytriidae sp.…”

Section: The Effect Of Dissolved Oxygencontrasting

confidence: 76%

Microbial production of docosahexaenoic acid by a low temperature‐adaptive strain Thraustochytriidae sp. Z105: screening and optimization

Zhou

et al. 2010

J. Basic Microbiol.

View full text Add to dashboard Cite

As an alternative source in addition to fish oil, microbial production of docosahexaenoic acid has been recieved more and more attentions owing to their culture advantage. A unicellular eukaryotic microbe with high DHA production and capable of low temperature-adaptive growth was isolated from seawater and identified as Thraustochytriidae sp. Z105. The siginificant effect of temperature on cell growth and DHA synthesis by the strain was revealed. It could grow and produce DHA even at 4 degrees C, but hardly grow above 35 degrees C. Low temperature (15-25 degrees C) was favorable for formation of biomass, lipids and DHA, but DHA synthesis was completely blocked above 30 degrees C. Conditions for high level DHA production by Thraustochytriidae sp. Z105 in flask culture were optimized as follows: medium containing glucose 80 g/l, yeast extract 5.0 g/l, K2HPO(4) . 3 H2O 1.0 g/l, MgSO4 . 7 H2O 0.5 g/l, seawater crystal 20 g/l, pH 6.0, liquid volume 30 ml/250 ml, temperature 20 degrees C, agitation speed of 200 r/min, and culture for 120 h. Under the optimal conditions, biomass of 16.72 g/l, total lipids of 5.35 g/l, DHA yield of 1.71 g/l (accounting for 32% of the total lipids) were achieved, respectively. In flask cluture level, the DHA productivity of Thraustochytriidae sp. Z105 was higher than most reported results, which suggested the wild type strain was a potential superior candidate for industrialization of DHA production. Moreover, the strain is an unique and valuable resource for investigation of the low temperature adaptive mechanism related to DHA synthesis.

show abstract

Production of high yields of docosahexaenoic acid byThraustochytrium roseum ATCC 28210

Cited by 58 publications

References 27 publications

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

Optimization of Culture Conditions for Growth and Docosahexaenoic Acid Production by a Marine Thraustochytrid, Aurantiochytrium limacinum mh0186

Untitled

Microbial production of docosahexaenoic acid by a low temperature‐adaptive strain Thraustochytriidae sp. Z105: screening and optimization

Contact Info

Product

Resources

About