Production of Human Rotavirus and Salmonella Antigens in Plants and Elicitation of fljB-Specific Humoral Responses in Mice

Bergeron-Sandoval, Louis-Philippe; Girard, Aurélie; Ouellet, François; Archambault, Denis; Sarhan, Fathey

doi:10.1007/s12033-010-9324-z

Cited by 9 publications

(1 citation statement)

References 41 publications

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“…Salmonella expresses two types of flagellin (FljB and FliC) known as strong immunogens. FljB has been expressed in plants, being capable to induce humoral responses in subcutaneously immunized mice (Bergeron-Sandoval et al 2011). The FliC protein flagellar subunit is an antigen capable to induce Th responses via macrophage-mediated presentation, stimulating antibody production; and it has become a candidate for vaccine development because it possesses a broad protective activity against multiple salmonella species (Cummings et al 2006;Cookson and Beavan 1997;McSorley et al 2000).…”

Section: Discussionmentioning

confidence: 99%

A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice

2019

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Key message A plant-based multiepitopic protein (LTBentero) containing epitopes from ETEC, S. typhimurium, and V. parahaemolyticus was produced in plants cells and triggered systemic and intestinal humoral responses in immunized mice. Abstract Around 200 million people suffer gastroenteritis daily and more than 2 million people die annually in developing countries due to such pathologies. Vaccination is an alternative to control this global health issue, however new low-cost vaccines are needed to ensure proper vaccine coverage. In this context, plants are attractive hosts for the synthesis and delivery of subunit vaccines. Therefore, in this study a plant-made multiepitopic protein named LTBentero containing epitopes from antigens of enterotoxigenic E. coli, S. typhimurium, and V. parahaemolyticus was produced and found immunogenic in mice. The LTBentero protein was expressed in tobacco plants at up to 5.29 µg g −1 fresh leaf tissue and was deemed immunogenic when administered to BALB/c mice either orally or subcutaneously. The plant-made LTBentero antigen induced specific IgG (systemic) and IgA (mucosal) responses against LTB, ST, and LptD epitopes. In conclusion, multiepitopic LTBentero was functionally produced in plant cells, being capable to trigger systemic and intestinal humoral responses and thus it constitutes a promising oral immunogen candidate in the fight against enteric diseases.

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Section: Discussionmentioning

confidence: 99%

A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice

2019

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Plant‐Made Vaccines Targeting Enteric Pathogens—Safe Alternatives for Vaccination in Developing Countries

Trujillo,

Angulo

2024

Biotech & Bioengineering

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Enteric diseases by pathogenic organisms are one of the leading causes of death worldwide, particularly in low‐income countries. Despite antibiotics, access to clean water and vaccination are the most economically affordable options to prevent those infections and their health consequences. Vaccines, such as those approved for rotavirus and cholera, have played a key role in preventing several enteric diseases. However, vaccines for other pathogens are still in clinical trials. Distribution and cost remain significant barriers to vaccine access in developing regions due to poor healthcare infrastructure, cold‐chain requirements, and high production costs. Plant‐made vaccines offer a promising alternative to address these challenges. Plants can be easily grown, lowering production costs, and can be administered in oral forms, potentially eliminating cold‐chain dependency. Although there are some promising prototypes of vaccines produced in plants, challenges remain, including yields and achieving sufficient immunogenicity. This review aims to describe common enteric pathogens and available vaccines, followed by a strategic summary of plant‐made vaccine development and a discussion of plant‐made enteric vaccine prototypes. Trends to overcome the key challenges for plant‐made vaccines are identified and placed in perspective for the development of affordable and effective vaccines for populations at the highest risk of enteric diseases.

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Biotechnological Interventions for the Production of Subunit Vaccines Against Group A Rotavirus

Prajapati,

Malik,

Sinha

et al. 2024

Cell Biochemistry & Function

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Group A rotavirus (RVA) is a major cause of severe gastroenteritis in infants and young children globally, despite the availability of live‐attenuated vaccines. Challenges such as limited efficacy in low‐income regions, safety concerns for immunocompromised individuals, and cold‐chain dependency necessitate alternative vaccine strategies. Subunit vaccines, which use specific viral proteins to elicit immunity, provide a safer and more adaptable approach. This review highlights biotechnological advancements in producing subunit vaccines, focusing on recombinant expression systems like bacterial, yeast, insect, mammalian, and plant‐based platforms for scalable and cost‐effective production of viral proteins. Key innovations include molecular engineering, adjuvant development, and delivery system improvements to enhance vaccine immunogenicity and efficacy. Subunit vaccines and virus‐like particles expressed in various systems have demonstrated promising preclinical and clinical results, with some candidates nearing commercial readiness. Reverse vaccinology, combined with Artificial Intelligence and Machine Learning, is driving the development of innovative multiepitope vaccines and antivirals. Strategies such as passive immunization, single‐chain antibodies, immunobiotics, and novel antivirals are also explored as alternative management options. The review also underscores advanced genome editing and reverse genetics approaches to improve vaccine design and antiviral therapies. These biotechnological interventions offer hope for equitable and effective control of rotavirus diarrhea, particularly in resource‐limited settings, and represent significant progress toward addressing current vaccine limitations.

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Production of Human Rotavirus and Salmonella Antigens in Plants and Elicitation of fljB-Specific Humoral Responses in Mice

Cited by 9 publications

References 41 publications

A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice

A multi-epitope plant-made chimeric protein (LTBentero) targeting common enteric pathogens is immunogenic in mice

Plant‐Made Vaccines Targeting Enteric Pathogens—Safe Alternatives for Vaccination in Developing Countries

Biotechnological Interventions for the Production of Subunit Vaccines Against Group A Rotavirus

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