Production of Curcuminoids in Different in vitro Organs of Curcuma longa

Abstract

Curcuma longa L. (turmeric) is one of the most important spice and safe food additives. Its main constituents, curcuminoids, showed anti-inflammatory, antitumor and antioxidant effects. In the present work, an in vitro propagation method was developed to achieve selected plant organs with quantified curcuminoid content. In vitro plants were obtained from sprouting buds as primary explants. The major curcuminoid constituents, such as curcumin (CUR), demethoxycurcumin (DEM), and bis-demethoxycurcumin (bis-DEM) were examined in different organs by LC-DAD-ESI-MS. A significant production of curcumin (more than 260 µg g-1 fresh weight) was obtained from in vitro microrhizomes, especially grown in a Murashige and Skoog medium (MS) supplemented with kinetin (0.1 mg L-1), -naphthaleneacetic acid (NAA, 1 mg L-1), sucrose (6%), agar (5%) and activated charcoal (0.1%). The analyzed microrhizomes showed reduced amounts of DEM and bis-DEM in comparison with CUR levels. In addition a shoot culture line was suitable to biosynthesize curcuminoids, in a ratio very similar to that identified in the fresh rhizomes of parent plants. This study represents the first direct quantification of curcuminoids in turmeric in vitro shoots and microrhizomes to be used in dietary supplements.

Microrhizome Induction and Curcumin Accumulation in Curcuma aromatica Salisb.

“…However, as shown in Table 9, although white turmeric microrhizomes may replace natural rhizomes, the curcumin levels in the five test samples from our study were much lower than those reported by Pistellia et al (2012) and Wu et al (2015). This might be due to genetic differences between our sample and those of other studies.…”

“…Curcuminoids also accumulate slowly during the development of white turmeric rhizomes (Baranowski, 1986). Moreover, changes in genotype, cultivation location, and processing procedures can result in changes in the chemical profile of commercial rhizomes (Pistellia et al, 2012). Therefore, the quantities of high-quality, genetically identical white turmeric rhizomes are insufficient for cultivation and subsequent extraction of metabolites.…”

A Comparative Investigation on Phenolic Composition, Characterization and Antioxidant Potentials of Five Different Australian Grown Pear Varieties

“…Some of the other compounds were reported in other plants but not pears. Pistelli et al [84] have identified the presence of demethoxycurcumin in Curcuma longa by LC-DAD-ESI-MS method. While the presence of isopimpinellin have also been confirmed by Dehghan et al [85] in Heracleum persicum.…”

Ozone-elicited secondary metabolites in shoot cultures of Melissa officinalis L.

“…In the last decade, many reports documented the use of plants as cell factories (Oksman-Caldentey and Inze ´2004) with the aim to increase the production of secondary metabolites in medicinal plants, e.g. as nutraceutical compounds (Pistelli et al 2012;Jacobo-Vela ´zquez and Cisneros-Zevallos 2012) or for other industrial purposes. However, the in vitro cultures require the optimization of growth and production conditions to maximize the yield of metabolites (Ruffoni et al 2010).…”