Production of industrial enzymes by filamentous fungi

Shahryari, Zohresadat; Niknezhad, Seyyed Vahid

doi:10.1016/b978-0-323-91872-5.00004-1

Cited by 1 publication

(1 citation statement)

References 134 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…On the other hand, the pelletised aggregation of mycelia reduces oxygen mass transfer and oxygen solubility and so increases the chances of oxygen deprivation inside the core of the pellets (Patel et al 2023 ). The hyphal aggregates are especially prone to shear stress damage because of the entanglement of the filamentous morphology when they are propagated in a bioreactor that has a high stirring speed (Shahryari and Niknezhad 2023 ). A continuous cultivation fermentation strategy could allow high throughputs if the fungus is able to sustain high dilution rates, but it has the drawback of a lower cell dry mass and it can only be sustained for a minimal retention time which can lead to a reduced total duration of the bioprocess (Patel et al 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Airlift bioreactor–based strategies for prolonged semi-continuous cultivation of edible Agaricomycetes

Cerrone,

Lochlainn,

Callaghan

et al. 2024

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Submerged cultivation of edible filamentous fungi (Agaricomycetes) in bioreactors enables maximum mass transfer of nutrients and has the potential to increase the volumetric productivity of fungal biomass compared to solid state cultivation. These aspects are paramount if one wants to increase the range of bioactives (e.g. glucans) in convenient time frames. In this study, Trametes versicolor (M9911) outperformed four other Agaricomycetes tested strains (during batch cultivations in an airlift bioreactor). This strain was therefore further tested in semi-continuous cultivation. Continuous and semi-continuous cultivations (driven by the dilution rate, D) are the preferred bioprocess strategies for biomass production. We examined the semi-continuous cultivation of T. versicolor at dilution rates between 0.02 and 0.1 h−1. A maximum volumetric productivity of 0.87 g/L/h was obtained with a D of 0.1 h−1 but with a lower total biomass production (cell dry weight, CDW 8.7 g/L) than the one obtained at lower dilution rates (12.3 g/L at D of 0.04 and vs 13.4 g/L, at a D of 0.02 h−1). However, growth at a D of 0.1 h−1 resulted in a very short fermentation (18 h) which terminated due to washout (the specific D exceeded the maximum growth rate of the fungal biomass). At a D of 0.04 h−1, a CDW of 12.3 g/L was achieved without compromising the total residence time (184 h) of the fermentation. While the D of 0.04 h−1 and 0.07 h−1 achieved comparable volumetric productivities (0.5 g/L/h), the total duration of the fermentation at D of 0.07 h−1 was only 85 h. The highest glucan content of cells (27.8 as percentage of CDW) was obtained at a D of 0.07 h−1, while the lowest glucan content was observed in T. versicolor cells grown at a D of 0.02 h−1. Key points • The highest reported volumetric productivity for fungal biomass was 0.87 g/L/h. • Semi-continuous fermentation at D of 0.02 h−1 resulted in 13.4 g/L of fungal biomass. • Semi-continuous fermentation at D of 0.07 h−1 resulted in fungal biomass with 28% of total glucans.

show abstract

Section: Introductionmentioning

confidence: 99%