Production of Infectious Hepatitis C Virus in Primary Cultures of Human Adult Hepatocytes

Podevin, Philippe; Carpentier, Arnaud; Pène, Véronique; Aoudjehane, Lynda; Carrière, Matthieu; Zaïdi, Sakina; Hernandez, Céline; Calle, Vanessa; Méritet, Jean–François; Scatton, Olivier; Dreux, Marlène; Cosset, François‐Loïc; Wakita, Takaji; Bartenschlager, Ralf; Demignot, Sylvie; Conti, Filoména; Rosenberg, Arielle R.; Calmus, Yvon

doi:10.1053/j.gastro.2010.06.058

Cited by 139 publications

(169 citation statements)

References 38 publications

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“…However, both kinds of particles are tightly associated with other lipoproteins, most notably ApoE and ApoC1. 174,175 These discrepancies are probably due to a defect of Huh7 cells to secrete ApoBcontaining VLDL particles, 173 supporting the notion of 'lipid imprinting' of HCV particles by the host cell.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 69%

“…162,[168][169][170][171][172] Moreover, the nature of the lipidic constituents of HCV particles appears to depend on the host cell in which the virus is produced. 173 This is best illustrated by the observation that cell culture grown HCV (HCVcc) produced in the human hepatoma cell line Huh7 differs from HCV circulating in patient serum as so-called lipoviro particles (LVPs). Both particle types have a heterogeneous density and in both cases density correlates inversely with infectivity.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%

“…Importantly, HCV produced in primary human hepatocytes also has a lower density as compared to HCVcc, closely resembling that of LVPs. 173 Another difference between HCVcc and LVPs relates to their propensity to associate with ApoB. While HCV particles present in infected livers can be immunoprecipitated with ApoB-specific antibodies, this capture is inefficient in case of Huh7-derived HCVcc.…”

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%

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Hepatitis c virus and host cell lipids: An intimate connection

Alvisi¹,

Madan²,

2011

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Section: O N O T D I S T R I B U T Ementioning

confidence: 69%

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%

Section: O N O T D I S T R I B U T Ementioning

confidence: 99%

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Hepatitis c virus and host cell lipids: An intimate connection

Alvisi¹,

Madan²,

2011

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“…In fact the much higher specific infectivity of HCV re-cultured from infected humanized mice compared to cell culture grown HCV is likely due to different particle properties of viruses grown in these environments [54]. HCV production in mice engrafted with a humanized liver [54], in PHH [91] or development of new cell lines fully competent for VLDL production is necessary to overcome this issue.…”

Section: Host Cells For the Investigation Of Hcv Entrymentioning

confidence: 99%

“…Permissive for HCVcc infection, with virus production reaching up to 10 4 -10 5 FFU/ml [91]. However, the low number of successful reports underscores the difficulty to work with this system.…”

Section: Namementioning

confidence: 99%

Entry and replication of recombinant hepatitis C viruses in cell culture

Vièyres

Pietschmann

2013

Methods

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Hepatitis C virus

Harris

2014

Encyclopedia of Life Sciences

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Hepatitis C virus is a positive‐sense RNA virus that is parenterally transmitted and is associated with chronic liver disease – fibrosis, cirrhosis, liver failure and hepatocellular carcinoma. The virus infects an estimated 123 million individuals worldwide with 5 million new infections occurring per annum. The prevalence of the virus, and the persistent nature of the infection, has led to the prediction that it will become an ever‐increasing burden on global healthcare resources. There is no vaccine to prevent infection; however, treatment options, until recently limited to a combination of interferon and ribavirin, have been improved by the advent of direct‐acting antivirals. These therapeutic advances have been underpinned by recent developments in our understanding of the molecular biology of virus infection, which are described in this article. In particular, the establishment of a system for the study of the complete virus lifecycle in tissue culture has led to many major advances and provides opportunities for further therapeutic developments. Key Concepts: Hepatitis C virus (HCV) is a major human pathogen infecting approximately 3% of the world population. HCV causes chronic liver disease – fibrosis, cirrhosis and hepatocellular carcinoma. HCV is a positive‐strand RNA virus that infects hepatocytes in the liver. Recent developments in HCV biology have allowed a deeper understanding of mechanisms of replication and pathogenesis. New direct‐acting antiviral compounds promise to improve therapeutic options.

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Production of Infectious Hepatitis C Virus in Primary Cultures of Human Adult Hepatocytes

Cited by 139 publications

References 38 publications

Hepatitis c virus and host cell lipids: An intimate connection

Hepatitis c virus and host cell lipids: An intimate connection

Entry and replication of recombinant hepatitis C viruses in cell culture

Hepatitis C virus

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