Production of Interleukin-1α by Human Endometrial Stromal Cells Is Triggered during Menses and Dysfunctional Bleeding and Is Induced in Culture by Epithelial Interleukin-1α Released upon Ovarian Steroids Withdrawal

Pretto, Chrystel; Chevronnay, Héloïse P. Gaide; Cornet, Patricia B.; Galant, Christine; Delvaux, Denis; Courtoy, Pierre J.; Marbaix, Étienne; Henriet, Patrick

doi:10.1210/jc.2007-2636

Cited by 24 publications

(19 citation statements)

References 38 publications

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“…However, menstruation starts in response to a drop in P concentration whereas proliferative explants barely contain P before culture. Tissue sampling for culture likely induces signaling cascades and/or allows the release of intracellular stocks of cytokines, such as IL-1␣, which is present in epithelial cells throughout the menstrual cycle and is able to stimulate MMP expression by stromal cells (6).…”

Section: Figmentioning

confidence: 99%

“…To further characterize the mechanisms controlling the local extent of endometrium remodeling during menstruation, we recently compared transcriptomes between degraded and preserved areas (4). This study highlighted major differences in gene expression profiles between stroma and glands, which could reflect or result from paracrine interactions between the two tissue compartments, as exemplified by the focal expression of stromal MMP-1 in response to epithelial IL-1␣ released at the onset of menstruation (3,5,6).…”

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confidence: 99%

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Ovarian Steroids, Mitogen-Activated Protein Kinases, and/or Aspartic Proteinases Cooperate to Control Endometrial Remodeling by Regulating Gene Expression in the Stroma and Glands

et al. 2010

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Explants from nonmenstrual endometria cultured in the absence of ovarian hormones undergo tissue breakdown. Addition of estradiol and progesterone (EP) prevents proteolysis. Explants include stromal and epithelial compartments which play different but complementary roles in endometrial physiology, including tissue remodeling and hormonal response. In order to characterize the cell type-specific contribution to regulation of tissue breakdown, we characterized the transcriptomes of microdissected stromal and glandular areas from endometrial explants cultured with or without EP. The datasets were also compared to other published endometrial transcriptomes. Finally, the contribution of proteolysis, hypoxia, and MAPKs to the regulation of selected genes was further investigated in explant culture. This analysis identified distinct gene expression profiles in stroma and glands, with differential response to EP, but functional clustering underlined convergence in biological processes, further indicating that endometrial remodeling requires cooperation between the two compartments through expression of cell type-specific genes. Only partial overlaps were observed between lists of genes involved in different occurrences of endometrial breakdown, pointing to a limited number of potentially crucial regulators but also to the requirement for additional mechanisms controlling tissue remodeling. We identified a group of genes differentially regulated by EP in stroma and glands among which some were sensitive to MAPKs and/or aspartic proteinases and were not induced by hypoxia. In conclusion, MAPKs and/or aspartic proteinases likely act in concert with EP to locally and specifically control differential expression of genes between degrading and preserved areas of the human endometrium.

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Section: Figmentioning

confidence: 99%

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Ovarian Steroids, Mitogen-Activated Protein Kinases, and/or Aspartic Proteinases Cooperate to Control Endometrial Remodeling by Regulating Gene Expression in the Stroma and Glands

et al. 2010

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“…First, the expression of several MMPs is controlled by EP (3) and by soluble local regulators such as IL-1␣ (4,5), LEFTY-A (6), and TGF-␤ (7). Second, being synthesized as inactive zymogens, MMPs must be activated by proteolytic propeptide removal (8).…”

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confidence: 99%

Metalloproteinase-Dependent Shedding of Low-Density Lipoprotein Receptor-Related Protein-1 Ectodomain Decreases Endocytic Clearance of Endometrial Matrix Metalloproteinase-2 and -9 at Menstruation

et al. 2009

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Cyclic elimination of the endometrium functional layer through menstrual bleeding results from intense tissue breakdown by proteolytic enzymes, mainly members of the matrix metalloproteinase (MMP) family. In contrast to menstrual-restricted MMPs, e.g. interstitial collagenase (MMP-1), gelatinases A (MMP-2) and B (MMP-9) mRNAs are abundant throughout the cycle without detectable tissue degradation at proliferative and secretory phases, implying a tight posttranslational control of both gelatinases. This paper addresses the role of low-density lipoprotein receptor-related protein (LRP)-1 in the endocytic clearance of endometrial gelatinases. LRP-1 mRNA and protein were studied using RT-PCR, Western blotting, and immunolabeling. Posttranslational control of LRP-1 was analyzed in explant culture. The receptor-associated protein (RAP), used as LRP antagonist, strongly increased (pro)gelatinase accumulation in medium conditioned by endometrial explants, suggesting a role for LRP-1 in their clearance. Although LRP-1 mRNA remained constant throughout the cycle, the protein ectodomain vanished at menses. LRP-1 immunolabeling selectively disappeared in areas of extracellular matrix breakdown in menstrual samples. It also disappeared from explants cultured without estrogen and progesterone (EP) due to ectodomain shedding in the medium. The shedding was inhibited by metalloproteinase inhibitors, including a disintegrin and metalloproteinase (ADAM) inhibitor, and by tissue inhibitors of MMPs (TIMP)-3 and -2, but barely by TIMP-1, pointing to ADAM-12 as the putative sheddase. In good agreement, ADAM-12 mRNA expression was repressed by EP. In conclusion, the efficient LRP-1-mediated clearance of gelatinase activity in nonbleeding endometrium is abrogated upon EP withdrawal, due to shedding of LRP-1 ectodomain by a metalloproteinase, presumably ADAM-12, itself regulated by EP.

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“…It has been demonstrated that secretion of MMP-1, MMP-3 and MMP-9 is stimulated by TNF-β and interleukin-1. In contrast, MMP-2 is independent of such cytokines [6,7].…”

Section: The Structure and Function Of Matrix Metalloproteinases And mentioning

confidence: 96%

The role of metalloproteinases in endometrial remodelling during menstrual cycle

et al. 2017

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Endometrium is the only tissue in the human body subject to cyclic transformations under the influence of ovarian steroid hormones. As estradiol and progesterone balance throughout the physiological menstrual cycle changes, so does the expression of metalloproteinases (MMPs). These endopeptides are responsible for keeping the balance between the process of synthesis and degradation of extracellular matrix proteins. Thus, MMP's take part in sustaining physiological stability of the endometrium. A number of MMPs found in the endometrial tissue and their activity is related to menstrual cycle phase. This paper is an up-to-date review of literature of Medline database. The search was conducted for key words including "matrix metalloproteinases", "MMPs", "TIMPs" and "tissue inhibitors of metalloproteinases". Over 1092 publications regarding interdependence and interplay between ovarian hormones and the role of various MMPs and their inhibitors in normal endometrial remodelling and in pathological conditions were analysed and critically reviewed.

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Production of Interleukin-1α by Human Endometrial Stromal Cells Is Triggered during Menses and Dysfunctional Bleeding and Is Induced in Culture by Epithelial Interleukin-1α Released upon Ovarian Steroids Withdrawal

Cited by 24 publications

References 38 publications

Ovarian Steroids, Mitogen-Activated Protein Kinases, and/or Aspartic Proteinases Cooperate to Control Endometrial Remodeling by Regulating Gene Expression in the Stroma and Glands

Ovarian Steroids, Mitogen-Activated Protein Kinases, and/or Aspartic Proteinases Cooperate to Control Endometrial Remodeling by Regulating Gene Expression in the Stroma and Glands

Metalloproteinase-Dependent Shedding of Low-Density Lipoprotein Receptor-Related Protein-1 Ectodomain Decreases Endocytic Clearance of Endometrial Matrix Metalloproteinase-2 and -9 at Menstruation

The role of metalloproteinases in endometrial remodelling during menstrual cycle

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