Production of iturin A by <i>Bacillus velezensis ND</i> and its biological control characteristics

Tang, Zhongmin; Cao, Xin; Zhang, Huili

doi:10.1002/jobm.202200473

Cited by 9 publications

(5 citation statements)

References 39 publications

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“…Similarly, the results of this study showed that B. velezensis BHZ-29 bacteria and a sterile fermentation broth had a strong inhibitory effect on V. dahliae , and could significantly improve the agronomic traits such as plant height, root length and root weight of cotton seedlings. Similarly to the B. velezensis ND ( Tang et al, 2023 ), B. velezensis BHZ-29 has a strong biocontrol potential by inhibiting the growth of V. dahliae , activating the disease resistance of the cotton system, enhancing the resistance of cotton to Verticillium wilt, and increasing cotton yield.…”

Section: Discussionmentioning

confidence: 99%

Optimization of the fermentation media and growth conditions of Bacillus velezensis BHZ-29 using a Plackett–Burman design experiment combined with response surface methodology

Shi,

Niu,

Yang

et al. 2024

Front. Microbiol.

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IntroductionBacillus velezensis occurs extensively in the soil environment. It produces a range of antimicrobial compounds that play an important role in the field of biological control. However, during the actual application process it is often affected by factors such as the medium formulation and fermentation conditions, and therefore biocontrol measures often do not achieve their expected outcomes.MethodsIn this study, the B. velezensis BHZ-29 strain was used as the research object. The carbon and nitrogen sources, and inorganic salts that affect the number of viable bacteria and antibacterial potency of B. velezensis BHZ-29, were screened by a single factor test. A Plackett–Burman design experiment was conducted to determine the significant factors affecting the number of viable bacteria and antibacterial potency, and a Box–Behnken design experiment was used to obtain the optimal growth of B. velezensis BHZ-29. The medium formula that produced the highest number of viable bacteria and most antibacterial substances was determined. The initial pH, temperature, amount of inoculant, liquid volume, shaking speed, and culture time were determined by a single factor test. The factors that had a significant influence on the number of viable bacteria of B. velezensis BHZ-29 were selected by an orthogonal test. A Box–Behnken design experiment was conducted to obtain the optimal fermentation conditions, and highest number of viable bacteria and antibacterial titer.ResultsMolasses, peptone, and magnesium sulfate had significant effects on the viable count and antibacterial titer of B. velezensis BHZ-29. The viable count of B. velezensis BHZ-29 increased from 7.83 × 109 to 2.17 × 1010 CFU/mL, and the antibacterial titer increased from 111.67 to 153.13 mm/mL when the optimal media were used. The optimal fermentation conditions for B. velezensis BHZ-29 were as follows: temperature 25.57°C, pH 7.23, culture time 95.90 h, rotation speed 160 rpm, amount of inoculant 2%, and liquid volume 100 ml. After the optimization of fermentation conditions, the number of viable bacteria increased to 3.39 × 1010 CFU/mL, and the bacteriostatic titer increased to 158.85 mm/ml.The plant height and leaf number of cotton plants treated with BHZ-29 fermentation broth were higher than those of cotton inoculated with Verticillium dahliae. The number of bacteria was 1.15 × 107 CFU/g, and the number of fungi was 1.60 × 105 spores/g. The disease index of the cotton seedlings treated with the optimized fermentation broth was 2.2, and a control effect of 93.8% was achieved. B. velezensis BHZ-29 could reduce the disease index of cotton Verticillium wilt and had a controlling effect on the disease. The best effect was achieved in the treatment group with an inoculation concentration of 2 × 108 CFU/ml, the disease index was 14.50, and a control effect of 84.18% was achieved.DiscussionThe fermentation process parameters of the number of viable bacteria and antibacterial titer by strain B. velezensis BHZ-29 were optimized to lay a foundation for the practical production and application of strain B. velezensis BHZ-29 in agriculture.

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Section: Discussionmentioning

confidence: 99%

Optimization of the fermentation media and growth conditions of Bacillus velezensis BHZ-29 using a Plackett–Burman design experiment combined with response surface methodology

Shi,

Niu,

Yang

et al. 2024

Front. Microbiol.

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“…The biological functional studies of B . velezensis 504 were performed as previously reported studies ( Tang et al., 2023 ). We detected the siderophore production from B .…”

Section: Methodsmentioning

confidence: 99%

Antagonistic transcriptome profile reveals potential mechanisms of action on Xanthomonas oryzae pv. oryzicola by the cell-free supernatants of Bacillus velezensis 504, a versatile plant probiotic bacterium

Zhou

et al. 2023

Front. Cell. Infect. Microbiol.

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Bacterial leaf streak (BLS) of rice is a severe disease caused by the bacterial pathogen Xanthomonas oryzae pv. oryzicola (Xoc) that has gradually become the fourth major disease on rice in some rice-growing regions in southern China. Previously, we isolated a Bacillus velezensis strain 504 that exhibited apparent antagonistic activity against the Xoc wild-type strain RS105, and found that B. velezensis 504 was a potential biocontrol agent for BLS. However, the underlying mechanisms of antagonism and biocontrol are not completely understood. Here we mine the genomic data of B. velezensis 504, and the comparative transcriptomic data of Xoc RS105 treated by the cell-free supernatants (CFSs) of B. velezensis 504 to define differentially expressed genes (DEGs). We show that B. velezensis 504 shares over 89% conserved genes with FZB42 and SQR9, two representative model strains of B. velezensis, but 504 is more closely related to FZB42 than SQR9, as well as B. velezensis 504 possesses the secondary metabolite gene clusters encoding the essential anti-Xoc agents difficidin and bacilysin. We conclude that approximately 77% of Xoc RS105 coding sequences are differentially expressed by the CFSs of B. velezensis 504, which significantly downregulates genes involved in signal transduction, oxidative phosphorylation, transmembrane transport, cell motility, cell division, DNA translation, and five physiological metabolisms, as well as depresses an additional set of virulence-associated genes encoding the type III secretion, type II secretion system, type VI secretion system, type IV pilus, lipopolysaccharides and exopolysaccharides. We also show that B. velezensis 504 is a potential biocontrol agent for bacterial blight of rice exhibiting relative control efficiencies over 70% on two susceptible cultivars, and can efficiently antagonize against some important plant pathogenic fungi including Colletotrichum siamense and C. australisinense that are thought to be the two dominant pathogenic species causing leaf anthracnose of rubber tree in Hainan province of China. B. velezensis 504 also harbors some characteristics of plant growth-promoting rhizobacterium such as secreting protease and siderophore, and stimulating plant growth. This study reveals the potential biocontrol mechanisms of B. velezensis against BLS, and also suggests that B. velezensis 504 is a versatile plant probiotic bacterium.

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“…Moreover, beyond its antibacterial activity, iturin A has been reported to possess insecticidal and anticancer properties (Wan et al 2022). Its extensive application prospects in food, medicine, and agriculture have garnered signi cant attention from researchers (Tang et al 2022).…”

Section: Introductionmentioning

confidence: 99%

“…Currently, researchers primarily employ single-product fermentation methods for the production of iturin A and vitamin K2, which not only escalate production costs but also result in resource wastage. The fabrication methods of iturin A are predominantly divided into three categories: solid-state fermentation (SSF) (Khedher et al 2021), submerged fermentation (SMF) (Tang et al 2022), and two-compartment bio lm reactor fermentation (Tang et al 2023). Tang et al (Tang et al 2022)compared the kinetic parameters of solid-state fermentation (SSF) and liquid deep fermentation (SMF) of Bacillus subtilis natto spores and proposed an innovative method for producing iturin A, achieving a yield of 12.46 g/kg through solid-state fermentation.…”

Section: Introductionmentioning

confidence: 99%

“…The fabrication methods of iturin A are predominantly divided into three categories: solid-state fermentation (SSF) (Khedher et al 2021), submerged fermentation (SMF) (Tang et al 2022), and two-compartment bio lm reactor fermentation (Tang et al 2023). Tang et al (Tang et al 2022)compared the kinetic parameters of solid-state fermentation (SSF) and liquid deep fermentation (SMF) of Bacillus subtilis natto spores and proposed an innovative method for producing iturin A, achieving a yield of 12.46 g/kg through solid-state fermentation. Moreover, Tang et al (Tang et al 2023) designed a two-compartment bio lm reactor, enabling a maximum itutin A concentration of 6.8 ± 0.1 g/L in fed-batch bio lm fermentation.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous Production of Vitamin K2 and Iturin A by Bacillus velezensis ND

Shen,

Cao,

Tang

et al. 2023

Preprint

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Bacillus velezensis is known to produce a diverse array of secondary metabolites, including the ability to synthesize lipopeptides such as iturin A, and additionally produce higher amounts of vitamin K2. Consequently, we sought to employ co-production fermentation to simultaneously generate vitamin K2 and iturin A. In order to significantly reduce fermentation costs and enhance substrate utilization efficiency, this article initially established a straightforward and effective product extraction method that enables the separate collection of vitamin K2 and iturin A from the fermentation broth. Simultaneously, the impacts of glycerol, soybean meal powder, yeast extract powder, and L-glutamic acid sodium, employed as carbon and nitrogen sources in the culture medium, on the co-production of vitamin K2 and iturin A were refined by optimizing factors such as culture temperature, initial pH and the concentrations of trace elements Na+ and K+, as well as additional additives L-glutamic acid sodium. It was discerned that glycerol, soybean meal powder, yeast extract powder, and L-glutamic acid sodium significantly influenced the concurrent production of vitamin K2 and iturin A. As a result, a novel process for the efficient co-production fermentation of vitamin K2 and iturin A was established, thereby enhancing the value-added aspect of the fermentation process. Furthermore, the implementation of a two-compartment biofilm reactor synergistically elevated the co-production efficiency. The yields of vitamin K2 and iturin A reached 46.88 mg/L and 5.58 g/L, respectively. This study provides a theoretical framework and practical implications for the integrated double-product fermentation process.

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Production of iturin A by Bacillus velezensis ND and its biological control characteristics

Cited by 9 publications

References 39 publications

Optimization of the fermentation media and growth conditions of Bacillus velezensis BHZ-29 using a Plackett–Burman design experiment combined with response surface methodology

Optimization of the fermentation media and growth conditions of Bacillus velezensis BHZ-29 using a Plackett–Burman design experiment combined with response surface methodology

Antagonistic transcriptome profile reveals potential mechanisms of action on Xanthomonas oryzae pv. oryzicola by the cell-free supernatants of Bacillus velezensis 504, a versatile plant probiotic bacterium

Simultaneous Production of Vitamin K2 and Iturin A by Bacillus velezensis ND

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