Production of L (+) Glutamic Acid in a Fully Membrane-Integrated Hybrid Reactor System: Direct and Continuous Production under Non-Neutralizing Conditions

Vikramachakravarthi, D.; Kumar, Ramesh; Pal, Parimal

doi:10.1021/ie503499w

Cited by 12 publications

(3 citation statements)

References 31 publications

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“…Generally, microfiltration and ultrafiltration membranes are used to recycle the mycelia and proteins back to fermenters, Moreover, nanofiltration, reverse osmosis and electrodialysis membrane are adopted in separating GA product instead of the filtration of ion exchange in conventional downstream processes. Vikramachakravarthi et al have used a neutral or charged nanofiltration membrane for the purification of GA and achieved a good result ( Vikramachakravarthi et al, 2014 ).…”

Section: Production Of Gluconic Acid and Its Derivatives By Fermentationmentioning

confidence: 99%

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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Gluconic acid (GA) and its derivatives, as multifunctional biological chassis compounds, have been widely used in the food, medicine, textile, beverage and construction industries. For the past few decades, the favored production means of GA and its derivatives are microbial fermentation using various carbon sources containing glucose hydrolysates due to high-yield GA production and mature fermentation processes. Advancements in improving fermentation process are thriving which enable more efficient and economical industrial fermentation to produce GA and its derivatives, such as the replacement of carbon sources with agro-industrial byproducts and integrated routes involving genetically modified strains, cascade hydrolysis or micro- and nanofiltration in a membrane unit. These efforts pave the way for cheaper industrial fermentation process of GA and its derivatives, which would expand the application and widen the market of them. This review summarizes the recent advances, points out the existing challenges and provides an outlook on future development regarding the production of GA and its derivatives by microbial fermentation, aiming to promote the combination of innovative production of GA and its derivatives with industrial fermentation in practice.

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Section: Production Of Gluconic Acid and Its Derivatives By Fermentationmentioning

confidence: 99%

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Zhang

et al. 2022

Front. Bioeng. Biotechnol.

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“…However, the genetic stability and production efficiency of production strains are still intrinsic to the industrialization of microbial glutamic acid production. Therefore, it is important to improve the genetic stability and increase the glutamic acid yield of production strains through mutagenic selection for the clean production of glutamic acid and increase the profit of enterprises [2].…”

Section: Introductionmentioning

confidence: 99%

Improved Glutamic Acid Production Capacity of Corynebacterium glutamicum by the ARTP Mutagenesis Method

et al. 2023

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Glutamic acid is an important amino acid that is used widely in the fields of food, medicine, and agriculture. One of the methods of glutamic acid production is direct microbial fermentation, so the genetic stability and glutamic-acid-producing capacity of the producing strain are the keys to improving glutamic acid concentration. Experiments were carried out using Corynebacterium glutamicum GL−6 as the parental strain, with two iterations of mutagenesis by atmospheric and room temperature plasma (ARTP) and screening with agar plates tolerant to high sugar and malonic acid, and the best strains with stable phenotypes were verified by fermentation in 20 L tanks. The results show that the optimal mutagenesis time of ARTP was 140 s, with lethality and positive mutation rates of 93.0% and 15.6%, respectively. The concentrations of the high-sugar and malonic acid agar plates were 240 g/L and 35 g/L, respectively. A mutant strain, P−45, with improved glutamic acid production capacity and genetic stability, was obtained through two rounds of iterative mutagenesis screening. The concentration of this strain in the Erlenmeyer flasks was 17.7 g/L, which was 18.8% higher than that of the parental strain, GL−6, and could be inherited stably for 10 generations. In the glutamic acid synthesis pathway, the upregulation of the gene encoding citrate synthase (cs), gene encoding isocitrate dehydrogenase (icdh), and gene encoding glutamate dehydrogenase (gdh), and the downregulation of the gene encoding oxoglutarate dehydrogenase complex (odhc) increased the carbon flows of the TCA cycle and its branch metabolic flow to glutamic acid synthesis. P−45 showed a glutamic acid concentration of 147.0 g/L under fed-batch fermentation conditions in 20 L tanks, which was 81.5% higher than the starting strain, GL−6. This study provides a new technical solution for improving microbial metabolites and genetic stability.

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“… 40 . Against the greatly explored batch or semi-batch processes involving multiple energy-intensive unit operations, the continuous production scheme which promise much higher productivity and higher acid concentration can be considered good alternatives 41 . In this context, the use of the porous liquid as the porous extraction medium, which has the potential to be incorporated in the continuous flow process 42 , is likely to generate versatile platforms for the isolation and purification of amino acid from the fermentation broth.…”

Section: Introductionmentioning

confidence: 99%

Type II porous ionic liquid based on metal-organic cages that enables l-tryptophan identification

et al. 2022

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Porous liquids with chemical separation properties are quite well-studied in general, but there is only a handful of reports in the context of identification and separation of non-gaseous molecules. Herein, we report a Type II porous ionic liquid composed of coordination cages that exhibits exceptional selectivity towards l-tryptophan (l-Trp) over other aromatic amino acids. A previously known class of anionic organic–inorganic hybrid doughnut-like cage (HD) is dissolved in trihexyltetradecylphosphonium chloride (THTP_Cl). The resulting liquid, HD/THTP_Cl, is thereby composed of common components, facile to prepare, and exhibit room temperature fluidity. The permanent porosity is manifested by the high-pressure isotherm for CH4 and modeling studies. With evidence from time-dependent amino acid uptake, competitive extraction studies and molecular dynamic simulations, HD/THTP_Cl exhibit better selectivity towards l-Trp than other solid state sorbents, and we attribute it to not only the intrinsic porosity of HD but also the host-guest interactions between HD and l-Trp. Specifically, each HD unit is filled with nearly 5 l-Trp molecules, which is higher than the l-Trp occupation in the structure unit of other benchmark metal-organic frameworks.

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Production of L (+) Glutamic Acid in a Fully Membrane-Integrated Hybrid Reactor System: Direct and Continuous Production under Non-Neutralizing Conditions

Cited by 12 publications

References 31 publications

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Production of Gluconic Acid and Its Derivatives by Microbial Fermentation: Process Improvement Based on Integrated Routes

Improved Glutamic Acid Production Capacity of Corynebacterium glutamicum by the ARTP Mutagenesis Method

Type II porous ionic liquid based on metal-organic cages that enables l-tryptophan identification

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