Production of l-lactic acid with immobilized Lactobacillus delbrueckii

Stenroos, Sirkka-Liisa; Linko, Yu‐Yen; Linko, P.

doi:10.1007/bf00144317

Cited by 77 publications

(13 citation statements)

References 6 publications

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“…The maximum and minimum process air flow rates through the CAH tube bank were 150 scfm (4.2 m 3 /min) and 120 scfm (3.4 m 3 /min), respectively. As ash deposits developed on the tube surfaces, heat recovery from the CAH tube bank decreased from maximum values of 41,200 -45,400 Btu/hr (43,466 to 47,897 kJ/hr), for inlet and outlet process air temperatures of 930° to 1010°F (499°-544°C) and 1220°-1270°F (660°-688°C) to a minimum of 18,300-23,200 Btu/hr (19,306 to 24,476 kJ/hr), for inlet and outlet process air temperatures of 695°-855°F (369°-458°C) and 830°-1000°F (444°-538°C), respectively. Figure 11 summarizes the CAH heat recovery data for the three coal-biomass-cofiring tests.…”

Section: Cah Resultsmentioning

confidence: 99%

Year 2 Biomass Utilization

Zygarlicke¹

2004

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This Energy & Environmental Research Center (EERC) Year 2 Biomass Utilization Final TechnicalReport summarizes multiple projects in biopower or bioenergy, transportation biofuels, and bioproducts. A prototype of a novel advanced power system, termed the high-temperature air furnace (HITAF), was tested for performance while converting biomass and coal blends to energy. Three biomass fuels-wood residue or hog fuel, corn stover, and switchgrass-and Wyoming subbituminous coal were acquired for combustion tests in the 3-million-Btu/hr system. Blend levels were 20% biomass-80% coal on a heat basis. Hog fuel was prepared for the upcoming combustion test by air-drying and processing through a hammer mill and screen. A K-Tron biomass feeder capable of operating in both gravimetric and volumetric modes was selected as the HITAF feed system. Two oxide dispersion-strengthened (ODS) alloys that would be used in the HITAF high-temperature heat exchanger were tested for slag corrosion rates. An alumina layer formed on one particular alloy, which was more corrosion-resistant than a chromia layer that formed on the other alloy. Research activities were completed in the development of an atmospheric pressure, fluidized-bed pyrolysis-type system called the controlled spontaneous reactor (CSR), which is used to process and condition biomass. Tree trimmings were physically and chemically altered by the CSR process, resulting in a fuel that was very suitable for feeding into a coal combustion or gasification system with little or no feed system modifications required. Experimental procedures were successful for producing hydrogen from biomass using the bacteria Thermotoga, a deep-ocean thermal vent organism. Analytical procedures for hydrogen were evaluated, a gas chromatography (GC) method was derived for measuring hydrogen yields, and adaptation culturing and protocols for mutagenesis were initiated to better develop strains that can use biomass cellulose. Fly ash derived from cofiring coal with waste paper, sunflower hulls, and wood waste showed a broad spectrum of chemical and physical characteristics, according to American Society for Testing and Materials (ASTM) C618 procedures. Higher-than-normal levels of magnesium, sodium, and potassium oxide were observed for the biomass-coal fly ash, which may impact utilization in cement replacement in concrete under ASTM requirements. Other niche markets for biomass-derived fly ash were explored. Research was conducted to develop/optimize a catalytic partial oxidation-based concept for a simple, low-cost fuel processor (reformer). Work progressed to evaluate the effects of temperature and denaturant on ethanol catalytic partial oxidation. A catalyst was isolated that had a yield of 24 mole percent, with catalyst coking limited to less than 15% over a period of 2 hours. In biodiesel research, conversion of vegetable oils to biodiesel using an alternative alkaline catalyst was demonstrated without the need for subsequent water washing. In work related to biorefinery technologies, ...

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Section: Cah Resultsmentioning

confidence: 99%

Year 2 Biomass Utilization

Zygarlicke¹

2004

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“…An effective recycling of 11 times was reported with a yield of 82% using cells of L delbrueckii immobilized in calcium alginate in a continuous PBCR. 29 Buyukgungor 30 found that L bulgaricus immobilized on k-carragenen beads could be recycled effectively four times with 100% conversion and a productivity of 0.83 g dm À3 h…”

Section: Operational Stability Of the Biocatalystmentioning

confidence: 99%

Chitosan‐treated polypropylene matrix as immobilization support for lactic acid production using Lactobacillus plantarum NCIM 2084

Kappalli

Gowthaman

Misra

et al. 2001

J of Chemical Tech & Biotech

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Adsorption coupled with electrostatic interaction as an immobilization technique is an important microbial cell immobilization technique. Treatment of the polymer matrix with the cationic surface treating agent chitosan for lactic acid production has been studied. Cells of Lactobacillus plantarum NCIM 2084 were immobilized on a polypropylene (PP) matrix treated with different concentrations of chitosan. The biocatalyst adsorbed on the 1.0 g dm À3 chitosan-treated PP matrix proved to be most effective. Repeated batch fermentation experiments showed that the immobilized biocatalyst could be recycled effectively 11 times. Studies were also carried out in a packed bed reactor with media recirculation. A high productivity of 7.66 g dm À3 h À1 could be obtained with a conversion of 94% and a yield of 97% at an average residence time of 30 h.

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“…Hence, even in a rigorously controlled batch fermentation the cells' efficiency of antibiotic production (specific productivity) begins to decline as early as [20][21][22][23][24][25] h after the induction of antibiotic production.' This specific productivity can be prolonged by the intermittant addition of essential nutrients, i.e., fed batch cultures,* by the periodic replacement of cells into a fresh medium, i.e., semicontinuous replacement cultures or by the continuous addition of fresh nutrients and the continuous removal of products (with or without cells), i.e., continuous-flow reactor culture^.^ The latter two procedures provide for a very effective way of maintaining a desired physical and metabolic environment in a culture vessel.…”

Section: Introductionmentioning

confidence: 99%

Semicontinuous and continuous production of penicillin‐G by Penicillium chrysogenum cells immobilized in κ‐carrageenan beads

Deo

Gaucher

1984

Biotech & Bioengineering

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Conidia of Penicillium chrysogenum were immobilized in K-carrageenan beads and then incubated in a growth-supporting medium to yield a penicillin producing immobilized cell mass. These in situ grown immobilized cells were used for the semicontinuous (replacement cultures)and continuous (fluidized bioreactor culture) production of penicillin-G. When periodically replaced into a minimal production medium, immobilized cells exhibited a half-life for penicillin production which was ninefold greater than that exhibited by free cells. The half-life of penicillin production and the yield of penicillin from glucose in such a replacement culture were greatly affected by the frequency of replacement and by the production medium's pH and concentration of glucose, phosphate, and trace metal nutrients. A penicillin-producing continuous flow bioreactor (150 mL), employing immobilized cells, was operated for up to 16 days. The best specific penicillin productivity (1.2 mg/g cells/h)yield from glucose (7.0 mg/g glucose) and half-life of production (15 days) were obtained when the feed medium contained 10 g/L of glucose, the pH was maintained at 7.0, the relative dissolved oxygen concentration was ca. 40%; and the residence time was 20 h.

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Production of l-lactic acid with immobilized Lactobacillus delbrueckii

Cited by 77 publications

References 6 publications

Year 2 Biomass Utilization

Year 2 Biomass Utilization

Chitosan‐treated polypropylene matrix as immobilization support for lactic acid production using Lactobacillus plantarum NCIM 2084

Semicontinuous and continuous production of penicillin‐G by Penicillium chrysogenum cells immobilized in κ‐carrageenan beads

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