Production of L-lysine by free and PVA-cryogel immobilizedCorynebacterium glutamicum cells

Велизаров, Светлозар; Rainina, E. I.; Синицын, А. П.; Varfolomeyev, S.D.; Lozinsky, V. I.; Zubov, A. L.

doi:10.1007/bf01022326

Cited by 31 publications

(11 citation statements)

References 9 publications

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“…The addition of cryoprotectants such as glycerol and skim milk helped stabilize activity. Velizarov et al (1992) demonstrated by measuring the level of intracelluiar ATP content that Corynebacterium glutamicum were partially deactivated during immobilization in PVA-cryogel. In this case, nongrowing cells were an advantage in achieving better conversion rates of glucose to lysine.…”

Section: Polyvinyl Alcohol (Pva)mentioning

confidence: 99%

Immobilization of Cells for Application in the Food Industry

Groboillot

Boadi

Poncelet

et al. 1994

Critical Reviews in Biotechnology

169

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Immobilization of cells offers advantages to the food process industries, including enhanced fermentation productivity and cell stability and reduced downstream processing costs due to facilitated cell recovery and recycle. This article summarizes the varied immobilization methodologies, including adsorption, entrapment, covalent binding, and microencapsulation. Examples of interest to the food industry are provided, together with a review of the physiological effects of immobilization. Topics in process engineering include immobilized cell bioreactor configurations and the scale-up potential of the various immobilization techniques.

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Section: Polyvinyl Alcohol (Pva)mentioning

confidence: 99%

Immobilization of Cells for Application in the Food Industry

Groboillot

Boadi

Poncelet

et al. 1994

Critical Reviews in Biotechnology

169

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“…After reusing the finished products in fermentation, the rate of immobilized cell decreased because some carriers were broken due to paddle and chemical components in fermentation medium. Beside, metabolic ability of immobilized cell in medium was different from that of free cell [4].…”

Section: Introductionmentioning

confidence: 91%

Optimization of Corynebacterium glutamicum immobilization process on bacterial cellulose carrier and its application for lysine fermentation

Tam¹

2014

IOSRJEN

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-Bacterial cellulose (BC) was used as a modern carrier in immobilization of cell. The immobilization of Corynebacterium glutamicum using bacterial cellulose (BC) as a carrier was carried out in two steps: adsorption and incubation. The screening method Plackett-Burman Design was used to identify the significantly influenced factors and the experiments were designed by Response Surface Methodology having the Central Composite Design (RSM-CCD). The obtained immobilization parameters were the cell density of 6.6 billion clone form units per milliliter, the weight of BC of 10g/100mL, the adsorption time of 6.82 hours with the shaking speed of 150 rpm and immobilized cell was incubated at 30 0 C for 3 days. The optimal efficiency of this immobilization reached 72.4% and the average density of cell on BC carrier was achieved 47.7 ± 0.02 billion clone form units per gram of finished product. These were fermented to receive L-lysine, the number reusing times of immobilized cell was eight, and the lysine field was 95% in the eighth time of reusing immobilized cell for fermentation with 26.032 ± 0.023 g/L and volumetric productivity was achieved 0.618 ± 0.100 g/(L.h). Furthermore, this immobile product was maintained under the suitable condition in the sterile water, pH = 7. After storing at 4 0 C for 30 days, the percentage of cell survival was 80%.

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“…Sato et al, 1982;Yamamoto et al, 1980Takamatsu et al, 1982Fujimara et al, 1984Kolot, 1981Sato et al, 1979Nishida et al, 1979;Seko et al, 1990Chibata et al, 1974Takamatsu etal., 1984a,b Yamada et al, 1980Slowinski and Charm, 1973Kim and Ryu, 1982Li etal., 1990Lu and Chen, 1988Constantinides et al, 1981Vieth and Venkatasubramanian, 1979Ogbonna et al, 1991a, b Galvan etal., 1990Chibata etal., 1974Wada etal., 1979 Velizarov et al, 1992Kanemitsu, 1975Nakamichi et al, 1989Tanakaetal., 1989Sirirote et al, 1988Marechal et al, 1979Bang et al, 1983daFonsecaetal., 1995Ishiwata et al, 1990 2.3.1.2 Production of Organic Acids Organic acids are extensively used in the food and pharmaceutical industries and some of them are products of microbial processes. Organic acids can be produced by gel immobilised microbial cells via bioconversion (cf .…”

Section: Production Of Amino Acidsmentioning

confidence: 99%