Production of L-ornithine from sucrose and molasses by recombinant Corynebacterium glutamicum

Zhang, Yuanyuan; Bu, Yifan; Liu, Jianzhong

doi:10.1007/s12223-014-0371-x

Cited by 23 publications

(4 citation statements)

References 30 publications

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“…Of late, de novo design of RBS sequences has been made possible by various computational tools that can predict the TIR of a given mRNA ORF as well as reverse engineer mRNA sequences for ORFs with a specified TIR. Two such tools, the RBS Calculator and the UTR Designer (Table ), ,, have been used successfully in multiple bacterial species for tuning protein expression levels across several orders of magnitude ,− (Figure A). They use similar models to predict the TIR of a given mRNA ORF by calculating the energy of interaction between the ribosome and the mRNA, incorporating contributions from energies of (a) rRNA:mRNA binding, (b) tRNA fMet :start-codon binding, (c) compensation for nonoptimal spacing between the SD and the start-codon, and (d) penalty due to 5′UTR structure that affects ribosome:mRNA interaction.…”

Section: Rna Parts and Toolsmentioning

confidence: 99%

Using RNA as Molecular Code for Programming Cellular Function

et al. 2016

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RNA is involved in a wide-range of important molecular processes in the cell, serving diverse functions: regulatory, enzymatic, and structural. Together with its ease and predictability of design, these properties can lead RNA to become a useful handle for biological engineers with which to control the cellular machinery. By modifying the many RNA links in cellular processes, it is possible to reprogram cells toward specific design goals. We propose that RNA can be viewed as a molecular programming language that, together with protein-based execution platforms, can be used to rewrite wide ranging aspects of cellular function. In this review, we catalogue developments in the use of RNA parts, methods, and associated computational models that have contributed to the programmability of biology. We discuss how RNA part repertoires have been combined to build complex genetic circuits, and review recent applications of RNA-based parts and circuitry. We explore the future potential of RNA engineering and posit that RNA programmability is an important resource for firmly establishing an era of rationally designed synthetic biology.

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Section: Rna Parts and Toolsmentioning

confidence: 99%

Using RNA as Molecular Code for Programming Cellular Function

et al. 2016

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“…Numerous microorganisms can utilize sucrose as a carbon source to synthesize valuable metabolites (Zhang et al 2018c , 2018e ; Zhao et al 2020 ). After intracellular transport and subsequent phosphorylation, sucrose can be converted to glucose phosphate and fructose by sucrose-6-phosphate hydrolase in C. glutamicum (Zhang et al 2015b ). Sucrose uptake is processed through a type II phosphotransferase system coupled with sugar phosphorylation (Martins et al 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Enhanced l-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum

Nie

Zhong

et al. 2022

Bioresour. Bioprocess.

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Abstractl-Ornithine, an important non-essential amino acid, has considerable medicinal value in the treatment of complex liver diseases. Microbial fermentation strategies using robust engineered strains have remarkable potential for producing l-ornithine. We showed that glucose and sucrose co-utilization accumulate more l-ornithine in Corynebacterium glutamicum than glucose alone. Further manipulating the expression of intracellular fructose-1-phosphate kinase through the deletion of pfkB1resulted in the engineered strain C. glutamicum SO30 that produced 47.6 g/L of l-ornithine, which represents a 32.8% increase than the original strain C. glutamicum SO26 using glucose as substrate (35.88 g/L). Moreover, fed-batch cultivation of C. glutamicum SO30 in 5-L fermenters produced 78.0 g/L of l-ornithine, which was a 78.9% increase in yield compared with that produced by C. glutamicum SO26. These results showed that manipulating the fructose metabolic pathway increases l-ornithine accumulation and provides a reference for developing C. glutamicum to produce valuable metabolites. Graphical Abstract

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“…The food crisis caused by the growing population has prompted researchers to explore more sustainable feedstocks to produce l -ornithine. Various carbon sources such as xylose [ 13 ], molasses [ 16 ], sucrose [ 17 ], glycerol [ 18 ], and arabinose [ 19 ] have been used as second-generation biomass feedstocks to produce l -ornithine. However, the obtained l -ornithine production titers have not exceeded 20 g/L, which severely limits the application of these carbon sources.…”

Section: Introductionmentioning

confidence: 99%

Improvement in l-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum

Nie

et al. 2022

Biotechnol Biofuels

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Background l-Ornithine is an important medicinal intermediate that is mainly produced by microbial fermentation using glucose as the substrate. To avoid competition with human food resources, there is an urgent need to explore alternative carbon sources for l-ornithine production. In a previous study, we constructed an engineered strain, Corynebacterium glutamicum MTL13, which produces 54.56 g/L of l-ornithine from mannitol. However, compared with the titers produced using glucose as a substrate, the results are insufficient, and further improvement is required. Results In this study, comparative transcriptome profiling of MTL01 cultivated with glucose or mannitol was performed to identify novel targets for engineering l-ornithine-producing strains. Guided by the transcriptome profiling results, we modulated the expression of qsuR (encoding a LysR-type regulator QsuR), prpC (encoding 2-methylcitrate synthase PrpC), pdxR (encoding a MocR-type regulator PdxR), acnR (encoding a TetR-type transcriptional regulator AcnR), CGS9114_RS08985 (encoding a hypothetical protein), and CGS9114_RS09730 (encoding a TetR/AcrR family transcriptional regulator), thereby generating the engineered strain MTL25 that can produce l-ornithine at a titer of 93.6 g/L, representing a 71.6% increase as compared with the parent strain MTL13 and the highest l-ornithine titer reported so far for C. glutamicum. Conclusions This study provides novel indirect genetic targets for enhancing l-ornithine accumulation on mannitol and lays a solid foundation for the biosynthesis of l-ornithine from marine macroalgae, which is farmed globally as a promising alternative feedstock.

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Production of L-ornithine from sucrose and molasses by recombinant Corynebacterium glutamicum

Cited by 23 publications

References 30 publications

Using RNA as Molecular Code for Programming Cellular Function

Using RNA as Molecular Code for Programming Cellular Function

Enhanced l-ornithine production from glucose and sucrose via manipulation of the fructose metabolic pathway in Corynebacterium glutamicum

Improvement in l-ornithine production from mannitol via transcriptome-guided genetic engineering in Corynebacterium glutamicum

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