Production of laccase from newly isolated Pseudomonas putida and its application in bioremediation of synthetic dyes and industrial effluents

Soil environment is a major sink for a multitude of chemicals and heavy metals, which inevitably leads to environmental contamination problems. Various human activities including agricultural, urban or industrial, or landfilling are major contributors to heavy metal contamination in the environment. Since landfilling is one of the ultimate waste disposal methods, the generation of leachate is inevitable. Leachate from landfill is highly heterogeneous and consist high amount of heavy metal. Subsequent movement of the leachate into the surrounding soil, ground water or surface water could lead to severe pollution problems to and cause toxicity to human and other living organisms. Microorganisms has the ability to solubilize the metals (or increase their bioavailability) via the production of siderophores and adsorb the metals in their biomass on metal-induced outer membrane proteins and by bio precipitation. Therefore this study aimed to remediate heavy metal in leachate contaminated soil from a closed non-sanitary landfill in Kuala Lumpur. Preliminary soil and leachate characterization revealed high amount of metal contaminants as compared to the prescribed limit by local and international standard. Total of eighteen microbes were isolated from the contaminated site and were grouped into two treatments, proteobacteria and non-proteo bacteria.Comparison between the treatments revealed that proteobacteria (Treatment A) were performing higher metal removal activity compared to non-proteobacteria (Treatment B) and control (Treatment C). Out of four metals tested in this study, three of the metals (As (71.86%), Ni (50.8%), Al (87.15%)) were removed significantly by the addition of Treatment A. Highest metal removal rate constant was obtained for Al at 0.02 day -1 . Therefore, it can be concluded that the addition of microbes, namely proteobacteria to leachate contaminated soil can remove the heavy metal content at a significant rate.

show abstract

“…It associated with in situ microbial community and suggests possible roles of these bacteria [9]. Fig.…”

Section: International Journal Of Bioscience Biochemistry and Bioinfmentioning

confidence: 92%

Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Fauziah¹,

Barasarathi²,

Emenike³

et al. 2017

IJBBB

View full text Add to dashboard Cite

show abstract

“…Maximum growth OD of 1.51 and maximum extracellular activity of 34.30 U/l was observed at pH: 8.0. Maximum enzyme production has been reported in other studies at an optimum pH of 8.0 such as in case of Pseudomonas putida MTCC 7525 [27] and γ-Proteobacterium JB [28].…”

Section: Optimization Of Culture Conditions For Maximum Laccase Produmentioning

confidence: 54%

“…The isolate exhibited maximum growth OD of 1.45 at a wavelength of 540nm after 96hrs, whereas maximum extracellular activity of 33.80 U/l was observed after 48hrs of the incubation period. In other reports, γ-proteobacterium JB produced maximum intracellular as well as an extracellular laccase activity after 48hrs of incubation, whereas maximum laccase production of 94.10 U/ml was recorded in Pseudomonas putida MTCC 7525 after 108hrs of incubation [27]. The selected bacteria showed a maximum growth OD of 1.632 and maximum extracellular activity of 33.70 U/l at an incubation temperature of 30 °C.…”

Section: Optimization Of Culture Conditions For Maximum Laccase Produmentioning

confidence: 61%

“…The selected bacteria showed a maximum growth OD of 1.632 and maximum extracellular activity of 33.70 U/l at an incubation temperature of 30 °C. In other studies of laccase production it was noted that, maximum enzyme production was achieved at 30 °C temperature in case of Pseudomonas putida MTCC 7525 [27] and at 37 °C in case of γ-proteobacterium JB [28]. Maximum growth OD of 1.51 and maximum extracellular activity of 34.30 U/l was observed at pH: 8.0.…”

Section: Optimization Of Culture Conditions For Maximum Laccase Produmentioning

confidence: 76%

See 1 more Smart Citation

Hyper-Production of Laccase By Pseudomonas putida LUA15.1 through Mutagenesis

Verma

Dhiman

Shirkot

2016

JMEN

View full text Add to dashboard Cite

“…Also, Mogharabi et al (2012) reported that laccase immobilized by gelatin alginate exhibited maximum removal of dye of about 80% amino black and eosin, which was similar to this study. Although, Kuddus et al (2013) showed P. putida MTCC 7525 laccase degraded Brilliant blue, Congo red and Crystal violet up to 74-93% at 24 h incubation which crossponds to 74.69% of T-Blue degradation by free laccase at 120 h incubation. Moreover, Kalme et al (2009) suggested that, presence of amino group represents the starching of pyrrol ring like structure at a peak of 1340 cmG 1 through FTIR, indicated that the some of the metabolites produced after decolourization.…”

Section: Discussionmentioning

confidence: 89%

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Narayanan¹,

Sevanan²,

Eva³

et al. 2015

Research J. of Microbiology

View full text Add to dashboard Cite

Laccases (benzenediol oxygen oxidoreductases, EC 1.10.3.2) are polyphenol oxidases (PPO) that catalyze the oxidation of various substituted phenolic compounds by using molecular oxygen as the electron acceptor. In the present study, Bacillus subtilis MTCC 2414 were employed for the production of laccase using guaiacol as a substrate under Submerged Fermentation (SmF) condition. Laccase was partially purified by salt precipitation method followed by dialysis. The synthetic dyes such as Orange 3R, Yellow GR and T-Blue were used for degradation studies using culture filtrate, free and immobilized laccases. The immobilized laccase obtained by entrapment method using sodium alginate was optimized and it exhibited maximum activity at pH 7 (321 U mLG 1 ) and temperature 35°C (317 U mLG 1 ). Similarly, free laccase was also optimized and the maximum activity was observed at pH 9 (309 U mLG 1 ) and temperature 35°C (339 U mLG 1 ). Surprisingly, Yellow GR dye was found to be highly degraded up to 81.72% by immobilized laccase when compared to free laccase (74.69% of T-Blue) and culture filtrate (72.16% of Orange 3R) respectively. The FTIR spectrum showed a spectrum peaks at 2110.12 c/m in control dye and degraded Yellow GR exposed peak at 3406.36 cmG 1 which represents C-N stretching of aromatic amine group in dyes. Therefore, high degradation ability of laccase from B. subtilis MTCC 2414 makes them attractive catalyst for biotechnological applications.

show abstract

Production of laccase from newly isolated Pseudomonas putida and its application in bioremediation of synthetic dyes and industrial effluents

Cited by 79 publications

References 25 publications

Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Remediation of Heavy Metal Contaminated Soil Using Potential Microbes Isolated from a Closed Disposal Site

Hyper-Production of Laccase By Pseudomonas putida LUA15.1 through Mutagenesis

Application of Immobilized Laccase from Bacillus subtilis MTCC 2414 on Decolourization of Synthetic Dyes

Contact Info

Product

Resources

About