2009
DOI: 10.1007/s00449-009-0341-x
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Production of lactic acid and ethanol by Rhizopus oryzae integrated with cassava pulp hydrolysis

Abstract: Cassava pulp was hydrolyzed with acids or enzymes. A high glucose concentration (>100 g/L) was obtained from the hydrolysis with 1 N HCl at 121 degrees C, 15 min or with cellulase and amylases. While a high glucose yield (>0.85 g/g dry pulp) was obtained from the hydrolysis with HCl, enzymatic hydrolysis yielded only 0.4 g glucose/g dry pulp. These hydrolysates were used as the carbon source in fermentation by Rhizopus oryzae NRRL395. R. oryzae could not grow in media containing the hydrolysates treated with 1… Show more

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Cited by 59 publications
(36 citation statements)
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References 43 publications
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“…This combination consists of 0.724 U/g cellulase, 1.458 U/g hemicellulase, 4.109 U/g pectinase and 5.702 U/g amylase. The DE of this study was higher than those were achieved by Sriroth [4] and Thongchul [14] but lower than those were achieved by Rattanachomsri [7]. Addition of mixture of different enzymes (2 U/g cellulase, 1.6 U/g hemicellulase, 4.9 U/g pectinase and …”
Section: Resultscontrasting
confidence: 69%
“…This combination consists of 0.724 U/g cellulase, 1.458 U/g hemicellulase, 4.109 U/g pectinase and 5.702 U/g amylase. The DE of this study was higher than those were achieved by Sriroth [4] and Thongchul [14] but lower than those were achieved by Rattanachomsri [7]. Addition of mixture of different enzymes (2 U/g cellulase, 1.6 U/g hemicellulase, 4.9 U/g pectinase and …”
Section: Resultscontrasting
confidence: 69%
“…This means that almost all starch in the cassava pulp was hydrolyzed to glucose. The maximum glucose yield obtained was higher than the result of cassava pulp hydrolysis using enzymes (70%) [13] or the combination of hydrothermal and enzymatic hydrolysis (75%) [18,19], but lower than the process reported by Thongchul, et al [30], who applied acid hydrolysis using hydrocholric acid, sulfuric acid and phosphoric acid with a high acid concentration (0.25-2 N) and conventional heating at 121°C, 15 psig for 15-60 minutes. The glucose yield gradually decreased when the heating temperature was further increased up to 230°C.…”
Section: The Effects Of Heating Temperaturementioning
confidence: 80%
“…This was perhaps due to the affinity of these inhibitors to ADH at the concentration studied could not compete with the enzyme-substrate affinity under the operating conditions during the growth phase [29]. Moreover, yeast extract present in the growth medium for promoting spore germination and initial cell growth favored ethanol production simultaneously [30].…”
Section: Resultsmentioning
confidence: 98%