Production of lignocellulolytic enzymatic complex using pretreated carnauba straw as carbon source and application on sugarcane bagasse hydrolysis

Silva, Francinaldo Leite da; Magalhães, Emilianny Rafaely Batista; Leitão, Ana Laura Oliveira de Sá; Santos, Everaldo Silvino dos

doi:10.1007/s13399-020-00815-w

“…Hence, the strain becomes potent producer of the lignocellulosic enzymes utilizing LCAWs as this study is rst to be reported for the high titer of cocktail of lignocellulolytic enzymes production with this strain. However, some authors stated the lignocellulolytic enzymes production using various LCAWs by several fungi [3,5,[11][12] and certain bacteria [10,21]. But none of these authors reported all the lignocellulolytic enzymes reported in this study.…”

Section: Optimization For the Cocktail Of Lignocellulolytic Enzymes Productionmentioning

confidence: 62%

“…Also Kerber et al, [5] reported the production of 53.6 U/mL of xylanase using barley bagasse which is lesser than that of our study. Nevertheless, Silva.et al, [12] reported Trichoderma reesei to secrete higher titer of xylanase (172 U/g dry substrate) and cellulase (2.4 U/g dry substrate) by usage of pretreated carnauba straw after 72 h of incubation. But the author fails to produce the remaining enzymes produced by Burkholderia sp SMB1and also the authors used pretreated substrate which is cost enhancing step.…”

Section: Optimization For the Cocktail Of Lignocellulolytic Enzymes Productionmentioning

confidence: 99%

“…However, there are several reports known till date on the isolation of the microbes viz. bacteria, fungi, actinomycetes, etc., producing lignocellulolytic enzymes [3,5,[10][11][12]. But to date there are no single repots on the isolation of Burkholderia sp for production of cocktail of these lignocellulolytic enzymes by usage of rice agro-residues like bran and straw as substrate.…”

Section: Introductionmentioning

confidence: 99%

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Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia Sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-Wastes for Bioethanol Production

Beladhadi

¹

,

Shankar

²

,

Jayalakshmi

³

et al. 2021

Preprint

0

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Purpose: This study focuses to assess the potentiality of the locally isolated Burkholderia sp SMB1. It was aimed for the cocktail of lignocellulolytic enzyme production involved in the depolymerization of the agro-wastes to release sugars for bioethanol production. Methods: The optimization of enzyme cocktail (lignolytic, cellulolytic, hemicellulolytic) production by isolated bacterium utilizing inexpensive substrates like bran and straw of rice was done using response surface methodology. Further this cocktail secreted by the bacterium at optimized conditions was employed for saccharifying untreated agro-wastes for sugars production which were fermented to bioethanol by S. cerevisiae.Results: This strain produced high titer of cellulase (10.8 U/mL), xylanase (76 U/mL), mannanase (14.23 U/mL), pectinase (62.18 U/mL) and laccase (24.25 U/mL) using rice bran at 7 pH and 40 oC using 10% (w/v) of rice bran. The production of cocktail of enzymes was slightly increased by adding CaCO3 (2mM) in the growth medium. This cocktail was able to hydrolyze untreated agro-wastes to release highest reducing sugars 28.5 g/L using untreated corn husk after 24 h of saccharification process. These sugars of corn husk were fermented by S. cerevisiae to produce highest 9.04 gL-1 bioethanol. Conclusion: This paper represents the exploitation of the locally isolated strain for the production of lignocellulolytic enzymes using rice bran and straw for the first time promising the novelty of the study. This study helps in waste disposal process by valorizing the waste biomass to produce value added products like sugars, bioethanol and hence increases agricultural economy.

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“…Hence, the strain becomes potent producer of the lignocellulosic enzymes utilizing LCAWs as this study is rst to be reported for the high titer of cocktail of lignocellulolytic enzymes production with this strain. However, some authors stated the lignocellulolytic enzymes production using various LCAWs by several fungi [3,5,[11][12] and certain bacteria [10,21]. But none of these authors reported all the lignocellulolytic enzymes reported in this study.…”

Section: Optimization For the Cocktail Of Lignocellulolytic Enzymes P...mentioning

confidence: 63%

“…However, there are several reports known till date on the isolation of the microbes viz. bacteria, fungi, actinomycetes, etc., producing lignocellulolytic enzymes [3,5,[10][11][12]. But to date there are no single repots on the isolation of Burkholderia sp for production of cocktail of these lignocellulolytic enzymes by usage of rice agro-residues like bran and straw as substrate.…”

Section: Introductionmentioning

confidence: 99%

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-wastes for Bioethanol Production

Beladhadi

¹

,

Shankar

²

,

Jayalakshmi

³

et al. 2021

Waste Biomass Valor

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Purpose: This study focuses to assess the potentiality of the locally isolated Burkholderia sp SMB1. It was aimed for the cocktail of lignocellulolytic enzyme production involved in the depolymerization of the agro-wastes to release sugars for bioethanol production.Methods: The optimization of enzyme cocktail (lignolytic, cellulolytic, hemicellulolytic) production by isolated bacterium utilizing inexpensive substrates like bran and straw of rice was done using response surface methodology. Further this cocktail secreted by the bacterium at optimized conditions was employed for saccharifying untreated agro-wastes for sugars production which were fermented to bioethanol by S. cerevisiae.Results: This strain produced high titer of cellulase (10.8 U/mL), xylanase (76 U/mL), mannanase (14.23 U/mL), pectinase (62.18 U/mL) and laccase (24.25 U/mL) using rice bran at 7 pH and 40 o C using 10% (w/v) of rice bran. The production of cocktail of enzymes was slightly increased by adding CaCO 3 (2mM) in the growth medium. This cocktail was able to hydrolyze untreated agro-wastes to release highest reducing sugars 28.5 g/L using untreated corn husk after 24 h of sacchari cation process. These sugars of corn husk were fermented by S. cerevisiae to produce highest 9.04 gL -1 bioethanol.Conclusion: This paper represents the exploitation of the locally isolated strain for the production of lignocellulolytic enzymes using rice bran and straw for the rst time promising the novelty of the study. This study helps in waste disposal process by valorizing the waste biomass to produce value added products like sugars, bioethanol and hence increases agricultural economy. Statement Of NoveltyThe production of the cocktail of lignolytic, cellulolytic and hemicellulolytic enzymes by the microorganisms has being the interesting topic because of its potentiality of utilizing the lignocellulosic agrowastes for the production of fermentable sugars. These sugars can be directly fermented to bioethanol without any detoxi cation as there may be less or no production of yeast inhibitors during the sacchari cation process through microbial or enzymatic process. Hence, the process becomes economically feasible as the steps required during fermentation (detoxi cation) may be reduced.Moreover, the isolated Burkholderia sp SMB1 becomes novel as it secretes high titer of enzymes after 24 h. This work represents to be the rst study on production of several lignocellulolytic enzymes by Burkholderia sp SMB1 strain utilizing several lignocellulosic agro-wastes. Thus, Burkholderia sp SMB1 strain shows the potentiality for its industrially application in biofuel industries as the cocktail of enzymes were utilized for the sacchari cation of agro-wastes to release fermentable sugars which are fermented to bioethanol by yeast strain.

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Solid-State Fermentation of Steam-Exploded Opuntia ficus-indica Cladodes Using Trichoderma reesei CCT-2768 for the Production of Cellulolytic Enzymes

Filho

¹

,

Santos

²

2022

Appl Biochem Biotechnol

0

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Production of lignocellulolytic enzymatic complex using pretreated carnauba straw as carbon source and application on sugarcane bagasse hydrolysis

Cited by 5 publications

References 40 publications

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia Sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-Wastes for Bioethanol Production

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia Sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-Wastes for Bioethanol Production

Production of Cocktail of Lignolytic, Cellulolytic and Hemicellulolytic Enzymes by the Novel Bacterium Burkholderia sp SMB1 Utilizing Rice Bran and Straw: Application in the Saccharification of Untreated Agro-wastes for Bioethanol Production

Solid-State Fermentation of Steam-Exploded Opuntia ficus-indica Cladodes Using Trichoderma reesei CCT-2768 for the Production of Cellulolytic Enzymes

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