Production of Lipase by Yarrowia lipolytica, I. Lipases from Yeasts (Review)

Hadeball, W.

doi:10.1002/abio.370110217

Cited by 40 publications

(26 citation statements)

References 51 publications

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“…The simultaneous production of microbial lipid and lipase has rarely been reported (Szczęsna- Antczak et al 2006). A wide range of microorganisms has been reported capable of producing lipases with different enzymological properties and substrate specificities (Hadeball, 1991;Dalmau et al 2000;Sharma et al 2001;Montesinos et al 2003;Szczęsna-Antczak et al 2006). Among them, various Y. lipolytica strains have been tested, with secretion of extracellular lipase being stimulated by the presence of longchain fatty acids or triacylglycerols as carbon source and organic nitrogen (i.e.…”

Section: Discussionmentioning

confidence: 99%

“…Traditionally, the most widespread biotechnological application of strains belonging to this species has focused on the production of single-cell protein (microbial biomass) (Barth and Gaillardin, 1996;Musiał et al 2004). In spite of the fact that species of the yeast Y. lipolytica were considered as good candidates for single-cell oil or lipase production (Hadeball, 1991;Ratledge, 1994), the production of these metabolites by Y. lipolytica strains has only recently been studied in detail. Wild or genetically engineered strains have recently been reported capable of storing huge quantities of intra-cellular lipid, located in lipid bodies, during growth on various hydrophobic materials (Papanikolaou et al 2001;Papanikolaou and Aggelis, 2003;Mličková et al 2004).…”

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confidence: 99%

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Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica

Papanikolaou¹,

Chevalot²,

Galiotou‐Panayotou³

et al. 2007

Electron. J. Biotechnol.

128

166

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Financial support: The present work was partially financed by the international cooperation project in industrial research activities of development in precompetitive stage-2005 (ΟΔΣΒΕΠΡΟ-69) entitled "Exploitation of the natural microflora of the olive fruit for the production of virgin oil of high quality" (General Secretariat of Research and Development of the Greek Ministry of Development -E.U.).

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Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica

Papanikolaou¹,

Chevalot²,

Galiotou‐Panayotou³

et al. 2007

Electron. J. Biotechnol.

128

166

View full text Add to dashboard Cite

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“…The average amount of linoleic acid in the corn oil was 56.31% of the total fatty acid content, while grape seed oil (60.45%) and sunflower oil (58.25%) were characterized by a higher concentration of this compound, and olive oil at 6.17% (C18:2 acid). Hadebal, 1991), and the main products of lipase-catalysed triglyceride hydrolysis were 2-monoacylglycerols and free fatty acids. The hypothesis was that high lipolytic activity would be associated with the content and type of fatty acids present at the sn-1 and sn-3 positions in the triacylglycerols.…”

Section: Resultsmentioning

confidence: 99%

Factors Influencing Synthesis of Extracellular Lipases by Yarrowia Lipolytica in Medium Containing Vegetable Oils

Fabiszewska¹,

Białecka‐Florjańczyk²

2014

J microb biotech food sci

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The aim of this study was to evaluate lipase activity of Yarrowia lipolytica KKP 379 in media containing selected vegetable oils as the sole carbon source. The highest activity for extracellular Yarrowia lipolytica lipases was obtained in a corn oil medium, which was almost two times higher compared to lipase activity in olive oil medium, while the lowest lipolytic activity was observed in medium containing rice and peanut oils. Literature suggested that free fatty acids, especially oleic acid present in olive oil, are good inducers of microbial lipase synthesis, however the results of this study do not support the hypothesis that high oleic acid content stimulates high lipolytic activity. Neither the relationship between the percentage of individual fatty acids in triacylglycerols of vegetable oils nor their content at the sn-1,3 positions influenced the activity of extracellular lipases synthesized by Yarrowia lipolytica KKP 379. Several hypothesis are given regarding the presence of other factors which may determine differences in extracellular lipolytic activity of yeast grown on different vegetable oils, such as presence of metal ions, the content of free fatty acids and critical micellar concentration.

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“…Isso pode ser explicado pelo fato de que emulsificadores como Tweens, mono e diacilgliceróis devem ser evitados na composição do meio de cultura, visto que sua presença no meio pode afetar a síntese e a atividade da lipase [17,18]. Segundo Colen (2006) [2], outro fator que pode dificultar a obtenção de enzimas lipolíticas é a ausência de peptona no meio de cultura.…”

Section: : Médias De Três Repetições Médias Seguidas Pela Mesma Letunclassified

Isolamento e seleção de micro-organismos produtores de enzimas de interesse comercial

Ribeiro¹,

Gois²,

Bispo³

et al. 2018

Sci. Plena

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Enzimas são proteínas amplamente utilizadas no âmbito industrial devido a sua alta especificidade de substrato e capacidade catalítica, sendo obtidas preferencialmente a partir de micro-organismos. Para suprimento dessa demanda, há uma crescente busca por novas linhagens de fungos e bactérias produtores potenciais dessas enzimas, levando ao isolamento a partir dos mais diferentes biomas. O manguezal possui uma grande biodiversidade associada às suas áreas. Por conta disso, foi utilizado para o presente trabalho o isolamento de micro-organismos a partir de um manguezal sergipano, avaliando posteriormente a capacidade dos isolados em produzir enzimas como amilases, proteases, celulases e lipases, que têm destaque nos setores farmacêuticos, de alimentos, têxtil e de bebidas. A produção dessas enzimas foi determinada através de seus índices enzimáticos usando a razão entre o diâmetro da degradação do indutor no meio e o diâmetro de crescimento microbiano. Nesse estudo, foi utilizado o método da difusão radial em meio sólido, onde foi observado que 65,5% das bactérias e 50% dos fungos isolados foram capazes de produzir enzimas amilolíticas, sendo 30% das bactérias consideradas boas produtoras com valores de índice enzimático ≥ 2,0. Com relação à produção de protease, 76,9 % das bactérias e 50% dos fungos isolados degradaram o meio proteico. No entanto, apenas 10% dos fungos e 69,2% das bactérias isoladas foram consideradas boas produtoras de protease. Os isolados obtidos não apresentaram atividades enzimáticas para celulase e lipase. Palavras-chave: enzimas, micro-organismo, isolamento Enzymes are proteins widely used in the industrial field due to their high substrate specificity and catalytic ability, being obtained preferably from microorganisms. To supply this demand, there is a growing search for new strains of potential fungi and bacteria producers of these enzymes, leading to isolation from the most different biomes. The mangrove has a great biodiversity associated to its areas. As a result, the isolation of microorganisms from a Sergipe mangrove was used for this study and, subsequently, the evaluation of the ability of the isolates to produce enzymes such as amylases, proteases, cellulases and lipases, which are prominent in the pharmaceutical, food, textile and beverage industries. The production of these enzymes was determined by their enzymatic index, which is the ratio between the diameter of the degradation of the inducer substance in the medium and the microbial growth one. In this study, it was used the radial diffusion method on solid medium, where it was observed that 65.5% of bacteria and 50% of the fungal isolates were able to produce amylolytic enzymes, with 30% of bacteria considered good enzyme producers with index values ≥ 2.0. Regarding protease production, 76.9% of bacteria and 50% of the fungal isolates degraded protein means. However, only 10% of fungi and 69.2% of bacteria isolates were found to be good protease producers. The obtained isolates did not present enzymatic activities for ...

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Production of Lipase by Yarrowia lipolytica, I. Lipases from Yeasts (Review)

Cited by 40 publications

References 51 publications

Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica

Industrial derivative of tallow: a promising renewable substrate for microbial lipid, single-cell protein and lipase production by Yarrowia lipolytica

Factors Influencing Synthesis of Extracellular Lipases by Yarrowia Lipolytica in Medium Containing Vegetable Oils

Isolamento e seleção de micro-organismos produtores de enzimas de interesse comercial

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