Production of lipid-containing algal-bacterial polyculture in wastewater and biomethanation of lipid extracted residues: Enhancing methane yield through hydrothermal pretreatment and relieving solvent toxicity through co-digestion

Bohutskyi, Pavlo; Phan, Duc; Spierling, Ruth; Kopachevsky, Anatoliy M.; Bouwer, Edward J.; Lundquist, Trygve; Betenbaugh, Michael J.

doi:10.1016/j.scitotenv.2018.11.026

Cited by 37 publications

(8 citation statements)

References 71 publications

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“…After the lipid extraction from algal-bacterial biomass, the leftover biomass residue is rich in carbohydrates and proteins, which makes anaerobic digestion a possible way for resource recovery []. The ultimate methane yield from anaerobic digestion of lipid-extracted biomass residue was 296 ± 2 mL/g VS, and hydrothermal pretreatment enhanced the methane production rate by 15–30% [ 233 ].…”

Section: Resource Recoverymentioning

confidence: 99%

A comprehensive review on the use of algal-bacterial systems for wastewater treatment with emphasis on nutrient and micropollutant removal

et al. 2022

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The scarcity of water resources and environmental pollution have highlighted the need for sustainable wastewater treatment. Existing conventional treatment systems are energy-intensive and not always able to meet stringent disposal standards. Recently, algal-bacterial systems have emerged as environmentally friendly sustainable processes for wastewater treatment and resource recovery. The algal-bacterial systems work on the principle of the symbiotic relationship between algae and bacteria. This paper comprehensively discusses the most recent studies on algal-bacterial systems for wastewater treatment, factors affecting the treatment, and aspects of resource recovery from the biomass. The algal-bacterial interaction includes cell-to-cell communication, substrate exchange, and horizontal gene transfer. The quorum sensing (QS) molecules and their effects on algal–bacterial interactions are briefly discussed. The effect of the factors such as pH, temperature, C/N/P ratio, light intensity, and external aeration on the algal-bacterial systems have been discussed. An overview of the modeling aspects of algal-bacterial systems has been provided. The algal-bacterial systems have the potential for removing micropollutants because of the diverse possible interactions between algae-bacteria. The removal mechanisms of micropollutants – sorption, biodegradation, and photodegradation, have been reviewed. The harvesting methods and resource recovery aspects have been presented. The major challenges associated with algal-bacterial systems for real scale implementation and future perspectives have been discussed. Integrating wastewater treatment with the algal biorefinery concept reduces the overall waste component in a wastewater treatment system by converting the biomass into a useful product, resulting in a sustainable system that contributes to the circular bioeconomy.

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Section: Resource Recoverymentioning

confidence: 99%

A comprehensive review on the use of algal-bacterial systems for wastewater treatment with emphasis on nutrient and micropollutant removal

et al. 2022

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“…The use of microalgae-bacteria consortia in these biorefineries is gaining more attention with respect to increasing microalgal productivity while improving economic feasibility [9]. For instance, Bohutskyi et al [118] evaluated the lipid production in an alga-bacteria polyculture grown in municipal primary wastewater and also evaluated the biomethane potential of the lipid extracted residues. The harvested biomass contained around 23% lipid content, including fatty acid methyl esters optimal for biodiesel production.…”

Section: Green Chemistry Projections and Circular Bioeconomymentioning

confidence: 99%

Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia

González-González¹,

de‐Bashan

2021

Biology

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Engineered mutualistic consortia of microalgae and bacteria may be a means of assembling a novel combination of metabolic capabilities with potential biotechnological advantages. Microalgae are promising organisms for the sustainable production of metabolites of commercial interest, such as lipids, carbohydrates, pigments, and proteins. Several studies reveal that microalgae growth and cellular storage of these metabolites can be enhanced significantly by co-cultivation with growth-promoting bacteria. This review summarizes the state of the art of microalgae–bacteria consortia for the production of microalgal metabolites. We discuss the current knowledge on microalgae–bacteria mutualism and the mechanisms of bacteria to enhance microalgae metabolism. Furthermore, the potential routes for a microalgae–bacteria biorefinery are outlined in an attempt to overcome the economic failures and negative energy balances of the existing production processes.

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“…For the yeast culture grown on primary sludge, the lipid productivity was a bit higher but with overall lower FAMEs content (0.4% (Zhang et al, 2014)). Groups working with primary treated wastewater showed lipid content ranging from 9 to 32% for strains of diverse microbial origin, however, the disclosed content of FAMEs did not exceed 0.3% of dry weight biomass (Bohutskyi et al, 2019;Hall et al, 2011;Ryu et al, 2014;Van Den Hende et al, 2011;Woertz et al, 2009) with the exception of Scenedesmus acutus, where the efficiency of the transesterification reaction was almost 90% (Table 1, (Sacristán de Alva et al, 2013). Microalgae grown on wastewater sampled from the aeration tank of the activated sludge process ('activated wastewater') showed lipid productivity of 0.1 g/L/day, with extractable FAMEs content of 11% from Chlorella sp.…”

Section: Oleaginous Microorganismsmentioning

confidence: 99%

Assessment of the production of biodiesel from urban wastewater-derived lipids

Frkova

Venditti

Herr

et al. 2020

Resources, Conservation and Recycling

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Production of biodiesel is one of the most important European targets within renewables for the future. To consider biodiesel a feasible alternative to fossil fuel, unconventional resources need to be exploited. This review aims to provide up-to-date knowledge on the existing reuse of lipids from urban wastewater to produce biodiesel. Lipids are readily removed by mixed microbial populations during wastewater treatments in sewage plants. Assessment results on potential annual European market supply indicate 3-414 10 4 tons (min for activated and max for grease trap sludge) of potentially extractable biodiesel from wastewater and an expected biodiesel demand of 14.8 10 6 tons. Considering the prospect of transforming sewage plants into biorefineries, we may cover on average 1.5, 6.2, 6.7 and 24.4% of activated, primary, scum and grease trap sludge respectively, of the European biodiesel market from wastewater-derived lipids. In addition, by implementing an optimized biotechnology selector, the overall biodiesel yield could be higher due to increased lipid incorporation into microbial biomass. This is not an insignificant amount and, if efficiently implemented, could represent an exploitable resource for biofuel production, an important and desired step towards a circular economy. The technology readiness level is still very low. There are several challenges and possible drawbacks, e.g., biogas yield loss, substrate depletion, or formation of floating sludge. Finally, no definitive legislative barriers towards wastewater-derived lipids have been identified; however, quality criteria as well as waste status have to be defined. Recently, many reports and regulations have been introducing circular economy principles in areas where sustainability of renewables is strongly considered. Generally, renewable sources having a high environmental impact such as indirect land use change due to crop-to-fuel cultivation, which needs to be better regulated. Accordingly, the limit

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Production of lipid-containing algal-bacterial polyculture in wastewater and biomethanation of lipid extracted residues: Enhancing methane yield through hydrothermal pretreatment and relieving solvent toxicity through co-digestion

Cited by 37 publications

References 71 publications

A comprehensive review on the use of algal-bacterial systems for wastewater treatment with emphasis on nutrient and micropollutant removal

A comprehensive review on the use of algal-bacterial systems for wastewater treatment with emphasis on nutrient and micropollutant removal

Toward the Enhancement of Microalgal Metabolite Production through Microalgae–Bacteria Consortia

Assessment of the production of biodiesel from urban wastewater-derived lipids

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