Production of Lipids and Proteome Variation in a Chilean Thraustochytrium striatum Strain Cultured under Different Growth Conditions

Shene, Carolina; Garcés, Marcelo; Vergara, Daniela; Peña, Jhonatan; Claverol, Stéphane; Rubilar, Mónica; Leyton, Allison

doi:10.1007/s10126-018-9863-z

Cited by 14 publications

(9 citation statements)

References 42 publications

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“…Its inadequate disposal could be an environmental threat, increasing the contamination of water and the uncontrolled release of methane. Thus, in recent years, the interest for converting residues and by-products of the food industry into products of added value has been promoted ( Thyagarajan et al., 2014 ; Silva et al., 2015 ; Caamaño et al., 2017 ; Shene et al., 2018 ; Villarroel and Silva, 2018 ; Sato et al., 2019 ; Kothri et al., 2020 ; Singh et al., 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…These microorganisms have been found in different marine and estuarine habitats including live algae, marine detritus, phytoplankton aggregates in invertebrates into the water column of the Mediterranean, Atlantic Ocean, the North Sea, Arabian Sea, coastal waters of Japan and India and also in coastal waters of Argentina and Chile ( Raghukumar, 2002 ; Raghukumar et al, 2000 , 2001 ; Naganuma et al, 1998 ; Bremer, 2000 ; Mo et al, 2002 ; Rosa et al, 2006 , 2011 ; Hinzpeter et al, 2009 ; Silva et al ., 2015 ; Pino , 2013 ; Shene et al, 2013 , 2018 ; Pino et al ., 2015 ; Gupta et al ., 2016 ; Caamaño et al, 2017 ). The TH can be isolated from various substrates and appear to be abundant in the sediments of coastal and estuarine environment ( Raghukumar & Raghukumar, 1999 ; Bongiorni, 1998 , 2012 ; Bongiorni et al, 2005 ; Singh et al, 2014 ; Caamaño et al, 2017 ).…”

Section: Introductionmentioning

confidence: 99%

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Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Quilodrán

Cortínez

Bravo

et al. 2020

Heliyon

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The thraustochytrid are marine heterotrophic protists that are widely distributed in the marine world. They are characterized by producing and accumulating great amount of lipids in their cells, especially long chain polyunsaturated fatty acids (LC-PUFA), highlighting the docosahexaenoic acid (DHA, 22:6, n-3), eicosapentaenoic acid (EPA, 20:5, n-3) and arachidonic acid (ARA, 20:4, n-6), as well as pigments of interest for human health and animal nutrition, such as carotenoids. Therefore, the objective of this study was to isolate and characterize three natives isolated of thraustochytrids and assess the potential of the by-products of the manufacture of beer (RB) and protein extraction of Lupine flour (RL) as complex carbon sources to produce biomass, lipid and polyunsaturated fatty acids. Three native strains of thraustochytrid (AS5-B2, IQ81 y VAL-B1), isolated from Chilean coastal waters were morphologically and genetically identified as thraustochytrid. For the determination of biomass production cultures were quantified by gravimetry and the fatty acids quantification and identification were carried out by gas chromatography (GC-FID). Our results show that the culture with any sources of complex carbon used, increased significantly the production of both biomass and total lipids in the strains IQ81 and VAL-B1, compared to glucose as pure carbon source. On the other hand, strain AS5-B2 showed a decrease in the total production of lipids in RB compared to the pure carbon source. For the production of fatty acids, the strains IQ81 and VAL-B1 showed a significant increase in DHA when growing in RB. In conclusion strains IQ81 and VAL-B1 can be used to biotransform industrial waste, such as RB and RL, into a more valuable product such as DHA, EPA, ARA and lipids.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Quilodrán

Cortínez

Bravo

et al. 2020

Heliyon

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“…KRS101 (19.88% TFA) [28] and Aurantiochytrium 4W-1b (27.9% TFA) [29]. The DHA content was also higher than those reported for genus Thraustochytrium (T. striatum) (0.8-6% TFA) [30] and Thraustochytrium sp. strain 004 (2.7% TFA) [31].…”

Section: Discussionmentioning

confidence: 68%

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Sirirak¹,

Suanjit²,

Powtongsook³

et al. 2020

ScienceAsia

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Aurantiochytrium limacinum BUCHAXM 122, a strain of thraustochytrid microorganism is potentially an alternative source of essential nutrients for commercial products. In this study, the strain was screened for its characterization and determination of polyunsaturated fatty acid (PUFA) production. Cells were isolated from fallen mangrove leaves, and grown in a glucose-yeast extract-peptone (GYP) medium. Their morphology, life cycle, biomass, fatty acid, extracellular enzymes and major elements were also investigated. Colonies occurred prominently on the fourth day of cultivation, appearing opaque white in color and containing large numbers of amoeboid cells. These thraustochytrids were observed to have a multi-stage life cycle, developing through zoospore, vegetative and zoosporangium stages with a life span of approximately 24 h. Zoospores developed into vegetative cells within 10-12 h and vegetative cells developed into zoosporangia, releasing zoospores, within approximately 10-15 h. Results showed that A. limacinum BUCHAXM 122 produced highest biomass at 108 h, with 23.85 ± 1.02 g/l dry weight. The isolates had high content of docosahexaenoic acid (DHA; 30.31 ± 5.88% of total fatty acids), indicating that they could serve as a fatty acid source for human consumption and aquaculture feeds. Major elemental analysis showed that cells contained 57% carbon, 29% oxygen, 9% hydrogen, 4% nitrogen and 1% sulfur. Extracellular degradative enzymes including protease, lipase, urease, α-glucosidase and phosphatase activities were also detected.

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“…It is acknowledged that Thraustochytrium sp. was able to grow on maltose, soluble starch (starch), and glucose; by contrast, fructose and sucrose did not support the growth (Shene et al, 2019) 2019), squalene content of thraustochytrids was the highest at 2% glucose but production per liter was not significantly different at concentrations from 2% to 6%. Therefore, glucose, maltose and starch at concentration of 2% were used to investigate the effect of carbon sources on cell growth and squalene production of the strain TN22 in this study (Fig.…”

Section: Carbon Source Testmentioning

confidence: 98%

Optimization of culture conditions for squalene production and squalene extraction method of <i>Thraustochytrium<\i> sp. TN22

Hien¹,

Thom²,

Ha³

et al. 2020

AJB

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Squalene, a natural triterpene with important roles as antioxidant, skin hydrating, cardio-protection and detoxifier, has attracted the attention of researchers in the world. This study investigated the optimal culture conditions for squalene production and to develop a method for squalene extraction and purification from cell suspension of Thraustochytrium sp. TN22. The results showed that squalene production by the strain Thraustochytrium sp. TN22 was optimum at 2% glucose, 0.5% yeast extract and 0,14% mixture of vitamins (B1, B6 and B12) at 28 oC for 2 days of cultivation. At the optimum conditions, the dry biomass, squalene content and productivity were approximately 9.3 g/L, 9.9 mg/g DCW and 95.3 mg/L, respectively. Time-dependence on cell lysis and extraction solvents were selected as the extraction parameters. The obtained results showed that the highest squalene production of 178.1 mg/100 g biomass was obtained in cell lysate at alkaline medium (pH = 10), at 60 oC for 6 hours and n-hexan was the best solvent for squalene extraction. Squalene was then purified on silica gel column using n-hexan as the mobile phase and 90% purity of squalene was archived. Our obtained results are promising for the commercial productions of various value-added compounds from species belong to the genus Thrautochytrium in Vietnam.

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Production of Lipids and Proteome Variation in a Chilean Thraustochytrium striatum Strain Cultured under Different Growth Conditions

Cited by 14 publications

References 42 publications

Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Characterization and comparison of lipid and PUFA production by native thraustochytrid strains using complex carbon sources

Characterization and PUFA production of Aurantiochytrium limacinum BUCHAXM 122 isolated from fallen mangrove leaves

Optimization of culture conditions for squalene production and squalene extraction method of <i>Thraustochytrium<\i> sp. TN22

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