Production of lysophosphatidic acid by lysophospholipase D in incubated plasma of spontaneously hypertensive rats and Wistar Kyoto rats

Tokumura, Akira; Fujimoto, Hiroaki; Yoshimoto, Osamu; Nishioka, Yuko; Miyake, Maki; Fukuzawa, Kenji

doi:10.1016/s0024-3205(99)00243-x

Cited by 56 publications

(58 citation statements)

References 25 publications

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“…LPA has not only been implicated in PM but also in normal vascular development [49], SMC and VSMC mitogenesis [50,51], and contractility [52,53]. Alkyl-LPA has been identified as a biologically active lipid in mildly oxidized low-density lipoprotein, human atherosclerotic lesions, and the supernatant of activated platelets [54].…”

Section: Discussionmentioning

confidence: 99%

The early- and late stages in phenotypic modulation of vascular smooth muscle cells: Differential roles for lysophosphatidic acid

Guo

Makarova

Cheng

et al. 2008

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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Lysophosphatidic acid (LPA) has been implicated as causative in phenotypic modulation (PM) of cultured vascular smooth muscle cells (VSMC) in their transition to the dedifferentiated phenotype. We evaluated the contribution of the three major LPA receptors, LPA 1 and LPA 2 GPCR and PPARγ, on PM of VSMC. Expression of differentiated VSMC-specific marker genes, including smooth muscle α-actin, smooth muscle myosin heavy chain, calponin, SM-22α, and hcaldesmon, was measured by quantitative real-time PCR in VSMC cultures and aortic rings kept in serum-free chemically defined medium or serum-or LPA-containing medium using wild-type C57BL/6, LPA 1 , LPA 2 , and LPA 1&2 receptor knockout mice. Within hours after cells were deprived of physiological cues, the expression of VSMC marker genes, regardless of genotype, rapidly decreased. This early PM was neither prevented by IGF-I, inhibitors of p38, ERK1/2, or PPARγ nor significantly accelerated by LPA or serum. To elucidate the mechanism of PM in vivo, carotid artery ligation with/without replacement of blood with Krebs solution was used to evaluate contributions of blood flow and pressure. Early PM in the common carotid was induced by depressurization regardless of the presence/absence of blood, but eliminating blood flow while maintaining blood pressure or after sham surgery elicited no early PM. The present results indicate that LPA, serum, dissociation of VSMC, IGF-I, p38, ERK1/2, LPA 1 , and LPA 2 are not causative factors of early PM of VSMC. Tensile stress generated by blood pressure may be the fundamental signal maintaining the fully differentiated phenotype of VSMC.

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Section: Discussionmentioning

confidence: 99%

The early- and late stages in phenotypic modulation of vascular smooth muscle cells: Differential roles for lysophosphatidic acid

Guo

Makarova

Cheng

et al. 2008

Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biolog

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“…The most important substances regulating porcine uterine contractions include: noradrenaline (NA), serotonine (Kitazawa et al 2001a), acetylcholine (Ach) (Kitazawa et al 2003), oxytocin (Carnahan et al 1999, Kitazawa et al 2001b, prostanoids (prostaglandin F 2 α, E 1 and E 2 ) (Kucharski et al 2007, Jana et al 2010, tromboxane A 2 , histamine (Kitazawa et al 1997), neuropeptide Y (Markiewicz et al 2003) and endothelin (Isaka et al 2000). Recently it has been documented that besides the above-mentioned substances, a representative of phospholipids, such as lysophosphatidic acid (1-acyl-sn-glycerol-3-phosphate-LPA) and its receptor (LPA 3 ) may play a significant role in reproductive processes including modulation of the uterine contractility (Tigyi et al 1992, Tokumura et al 1999, Budnik and Mukhopadhyay 2002. LPA is a multifunctional lipid messenger and is the simplest of all glycerophospholipids (Moolenaar 1995).…”

Section: Introductionmentioning

confidence: 99%

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Markiewicz¹,

Kamińska²,

Bogacki³

et al. 2012

Polish Journal of Veterinary Sciences

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Recent studies show that a representative of phospholipids, namely lysophosphatidic acid (LPA) and its receptors (LPA 1-3 ) play a significant role in the reproductive processes, i. a, in the modulation of the uterine contractility. The participation of LPA 3 in the reproductive processes has been revealed in mice and has not been studied in gilts. Therefore, in the present study we investigated the role/action of LPA and its receptors LPA 1 , LPA 2 and LPA 3 on the contraction activity in the porcine uterus. The study was conducted on an experimental model in which the pig uterus consisted of the one whole uterine horn and a part of the second horn, both connected with the uterine corpus. Uterine strips consisting of the endometrium with the myometrium (ENDO/MYO) and myometrium (MYO) alone were collected on days 12-14 of the estrous cycle (control group; n = 5) or pregnancy (experimental group; n = 5). Two analogues of LPA at increasing doses were used: oleoyl-sn-glycero-3-phosphate (L-α-LPA, a selective agonist of LPA 1 and LPA 2 receptors; 10 -7 M; 10 -6 M and 10 -5 M) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT, a selective agonist of LPA 3 receptor; 68 nM; 136 nM and 680 nM). L-α-LPA caused an increase in the contraction tension, amplitude and frequency of ENDO/MYO from the uterine horn with the developing embryos. This effect was not observed in MYO in both groups examined. In the ENDO/MYO strips of the uterine horn with developing embryos, OMPT significantly increased the contraction tension at the highest dose (680 nM) and amplitude at all doses examined, while frequency of contractions was decreased at doses of 136 nM and 680 nM. In the MYO strips of the uterine horn with embryos a significant increase in the contraction tension and amplitude after the highest dose of OMPT was observed. The results obtained imply the important role of receptors LPA 1 , LPA 2 and LPA 3 in the contraction activity of the porcine uterus during early pregnancy.

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“…55: [393][394][395][396][397][398][399] 2009) ysophosphatidic acid (LPA), a simple phospholipid, has a vast variety of physiological and pathological actions, such as cell proliferation and differentiation [1,2], cytoskeletal rearrangement [3], cell-to-cell interactions [4] and tumorigenesis [5,6]. In the literature, at least two pathways of LPA synthesis have been postulated [7,8]. In serum and plasma, LPA is mainly converted from lysophospholipids [8].…”

mentioning

confidence: 99%

“…In the literature, at least two pathways of LPA synthesis have been postulated [7,8]. In serum and plasma, LPA is mainly converted from lysophospholipids [8]. By contrast, in platelets and some cancer cells, LPA is converted from phosphatidic acid.…”

mentioning

confidence: 99%

Lysophosphatic Acid Modulates Prostaglandin Secretion in the Bovine Endometrial Cells Differently on Days 8-10 of the Estrous Cycle and Early Pregnancy

Wocławek-Potocka

Brzezicka

Skarzynski

2009

Journal of Reproduction and Development

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Abstract. Lysophosphatidic acid (LPA) is becoming a new player in regulation of the reproductive processes of domestic animals. In the present study, we examined whether LPA modulates prostaglandin (PG) synthesis in the bovine endometrium at the time of the early maternal pregnancy recognition compared with the respective days of the estrous cycle and the enzymatic mechanism of this action. Bovine epithelial and stromal endometrial cells isolated from the uteri on days 8-10 of the estrous cycle and pregnancy were cultured with LPA for 24 h. LPA increased PGE2 production in stromal cells during the estrous cycle and early pregnancy. On days 8-10 of pregnancy, LPA inhibited PGF2α production in epithelial cells. LPA stimulated PGES mRNA expression in stromal cells during both examined phases and inhibited PGFS mRNA expression in epithelial cells on days 8-10 of pregnancy. The overall results indicate that LPA may serve as a luteotropic factor during the luteal phase of the estrous cycle and early pregnancy stimulating PGE2 synthesis and mRNA expression for PGES in stromal cells. Moreover, during early pregnancy, LPA might protect bovine CL and early embryo development by decreasing PGF2α synthesis and mRNA expression for PGFS in the epithelial cells of the bovine endometrium. Key words: Cow, Lysophosphatidic acid, Prostaglandins, Uterus (J. Reprod. Dev. 55: [393][394][395][396][397][398][399] 2009) ysophosphatidic acid (LPA), a simple phospholipid, has a vast variety of physiological and pathological actions, such as cell proliferation and differentiation [1,2], cytoskeletal rearrangement [3], cell-to-cell interactions [4] and tumorigenesis [5,6]. In the literature, at least two pathways of LPA synthesis have been postulated [7,8]. In serum and plasma, LPA is mainly converted from lysophospholipids [8]. By contrast, in platelets and some cancer cells, LPA is converted from phosphatidic acid. In each pathway, at least two phospholipase activities are required; phospholipase A1 (PLA1)/PLA2 plus lysophospholipase D (lysoPLD) activities are involved in the first pathway, and phospholipase D (PLD) plus PLA1/PLA2 activities are involved in the second pathway [7]. In the reproductive system, the LPA role has been studied extensively so far. We have recently found that in the bovine endometrium, unlike in mice, pigs and sheep [9][10][11][12], there is only mRNA expression for the receptor for LPA type 1 (LPA1) [13]. We also found that LPA is locally produced and released from the bovine endometrium [13]. The lysophosphatidic acid concentration and expression of mRNA for LPA1 in the bovine endometrium are significantly higher during early pregnancy than during the estrous cycle. Moreover, LPA stimulates progesterone (P4) and prostaglandin (PG) E2 secretion in vivo, and its receptor gene expression (LPA1) is positively correlated with expression of the enzyme responsible for luteotropic PGE2 production (PGES) in the bovine endometrium during the estrous cycle and early pregnancy. These data indicate that LPA may play auto...

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Production of lysophosphatidic acid by lysophospholipase D in incubated plasma of spontaneously hypertensive rats and Wistar Kyoto rats

Cited by 56 publications

References 25 publications

The early- and late stages in phenotypic modulation of vascular smooth muscle cells: Differential roles for lysophosphatidic acid

The early- and late stages in phenotypic modulation of vascular smooth muscle cells: Differential roles for lysophosphatidic acid

Participation of analogues of lysophosphatidic acid (LPA): oleoyl-sn-glycero-3-phosphate (L-α-LPA) and 1-oleoyl-2-O-methyl-rac-glycerophosphothionate (OMPT) in uterine smooth muscle contractility of the pregnant pigs

Lysophosphatic Acid Modulates Prostaglandin Secretion in the Bovine Endometrial Cells Differently on Days 8-10 of the Estrous Cycle and Early Pregnancy

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