Production of male and female offspring in the strictly monogenic fly Chtysomya rufifacies after ovary transplantation

Ullerich, Fritz-Helmut

doi:10.1007/bf00438313

Cited by 4 publications

(4 citation statements)

References 5 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Are feminizing cytoplasmic genes to be suspected in crustaceans other than amphipods and isopods ? There are reports of monogeny in other crustaceans however, in for instance Triops (Branchiopoda : Notostraca), monogeny appears to be controlled, as it is in the dipteran Chryosomya (Ullerich, 1975(Ullerich, , 1977(Ullerich, , 1982, by nuclear genes inherited in a Mendelian fashion (Sassaman, 1989(Sassaman, , 1991 (see also Sassaman, 1988, on Eulimnodia antlei). Feminization of males by protozoans is however indicated by the work of Smith (1905) on the decapod spider crab (Inachus dorsettensis).…”

Section: (C) Other Crustaceansmentioning

confidence: 99%

The Incidences. Mechanisms and Evolution of Cytoplasmic Sex Ratio Distorters in Animals

1993

View full text Add to dashboard Cite

Section: (C) Other Crustaceansmentioning

confidence: 99%

The Incidences. Mechanisms and Evolution of Cytoplasmic Sex Ratio Distorters in Animals

1993

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

“…The hairy maggot blow fly, Chrysomya rufifacies Macquart (Diptera: Calliphoridae), has an unusual sex-determination system (monogeny) that makes it uniquely well suited to the study of sexual dimorphism throughout development (Wilton, 1954;Ullerich, 1977). In this species, females produce genetically pre-determined single-sex offspring clutches, independent of the zygotic genome of the offspring (Ullerich, 1963(Ullerich, , 1973(Ullerich, , 1975(Ullerich, , 1977(Ullerich, , 1980(Ullerich, , 1984. Female-producing (thelygenic) females are hypothesized to be heterozygote dominant for a factor that they incorporate into their eggs that causes their offspring to develop into fertile females (Ullerich, 1963(Ullerich, , 1973(Ullerich, , 1975(Ullerich, , 1977(Ullerich, , 1980(Ullerich, , 1984, while male-producing (arrhenogenic) females and males are hypothesized to be homozygous recessive for this same factor.…”

Section: Introductionmentioning

confidence: 99%

“…In this species, females produce genetically pre-determined single-sex offspring clutches, independent of the zygotic genome of the offspring (Ullerich, 1963(Ullerich, , 1973(Ullerich, , 1975(Ullerich, , 1977(Ullerich, , 1980(Ullerich, , 1984. Female-producing (thelygenic) females are hypothesized to be heterozygote dominant for a factor that they incorporate into their eggs that causes their offspring to develop into fertile females (Ullerich, 1963(Ullerich, , 1973(Ullerich, , 1975(Ullerich, , 1977(Ullerich, , 1980(Ullerich, , 1984, while male-producing (arrhenogenic) females and males are hypothesized to be homozygous recessive for this same factor. Furthermore, as C. rufifacies has homomorphic sex chromosomes, maternally determined sex, and limited genetic data available, it is not currently possible to use karyotyping or genome-size for immature sex-identification (Ullerich, 1973(Ullerich, , 1975Picard et al, 2012;Andere et al, 2020).…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Sexual Dimorphism in Growth Rate and Gene Expression Throughout Immature Development in Wild Type Chrysomya rufifacies (Diptera: Calliphoridae) Macquart

et al. 2021

View full text Add to dashboard Cite

Reliability of forensic entomology analyses to produce relevant information to a given case requires an understanding of the underlying arthropod population(s) of interest and the factors contributing to variability. Common traits for analyses are affected by a variety of genetic and environmental factors. One trait of interest in forensic investigations has been species-specific temperature-dependent growth rates. Recent work indicates sexual dimorphism may be important in the analysis of such traits and related genetic markers of age. However, studying sexual dimorphic patterns of gene expression throughout immature development in wild-type insects can be difficult due to a lack of genetic tools, and the limits of most sex-determination mechanisms. Chrysomya rufifacies, however, is a particularly tractable system to address these issues as it has a monogenic sex determination system, meaning females have only a single-sex of offspring throughout their life. Using modified breeding procedures (to ensure single-female egg clutches) and transcriptomics, we investigated sexual dimorphism in development rate and gene expression. Females develop slower than males (9 h difference from egg to eclosion respectively) even at 30°C, with an average egg-to-eclosion time of 225 h for males and 234 h for females. Given that many key genes rely on sex-specific splicing for the development and maintenance of sexually dimorphic traits, we used a transcriptomic approach to identify different expression of gene splice variants. We find that 98.4% of assembled nodes exhibited sex-specific, stage-specific, to sex-by-stage specific patterns of expression. However, the greatest signal in the expression data is differentiation by developmental stage, indicating that sexual dimorphism in gene expression during development may not be investigatively important and that markers of age may be relatively independent of sex. Subtle differences in these gene expression patterns can be detected as early as 4 h post-oviposition, and 12 of these nodes demonstrate homology with key Drosophila sex determination genes, providing clues regarding the distinct sex determination mechanism of C. rufifacies. Finally, we validated the transcriptome analyses through qPCR and have identified five genes that are developmentally informative within and between sexes.

show abstract

Analysis of the predetermining effect of a sex realizer by ovary transplantations in the monogenic flyChrysomya rufifacies

Ullerich

1980

Wilhelm Roux' Archiv

View full text Add to dashboard Cite

In the heterozygousF'/f female-producing females of the strictly monogenic blowflyChrysomya rufifacies the gene product of the dominant or epistatic female sex realizerF' which causes sexpredetermination is thought to be synthesized either by cells of the germ line (oocytes, nurse cells or oogonia) or by somatic cells and then transferred into the oocytes. To determine the possible site of synthesis, reciprocal transplantations were made of prepupal ovaries between female-producing (thelygenic; t) and male-producing (arrhenogenic; a) females ofChrysomya rufifacies. In another series of experiments prepupal host females of the wild t-type and a-type were each provided with one additional ovary either from a0type (f/f) or a t-type (F'/f) prepupa (neither were distinguishable by their phenotypes). In all these experiments the donor females were marked by the recessive sex-linked mutation 'white' (w/w); white eyes, white Malpighian tubules). In a considerable number of cases the implanted ovaries were in contact with the host's own oviduct and grew normally, but the rate of hatched adults was somewhat reduced. Crosses between such host females andw/w males (f/f) produced female or male offspring with white eyes from the eggs of the implantedw/w ovary, as well as flies with wild-type eyes (+/w) which had developed in the host's own ovaries. In all cases so far examined, the genetically thelygenic (or arrhenogenic) host females with an additional ovary implanted from an arrhenogenic (thelygenic) donor, produced progeny of both sexes: sons (daughters) from the eggs of the donor's ovary and daugthers (sons) from the eggs of the host's own gonads.These results demonstrate that the ovaries of the t-females ofChrysomya rufifacies at least from the early prepupal stage, are autonomous for the product of theF' gene. Transplantations of the premordial germ cells (pole cells) are planned to find out whether the predeterminingF' gene product is synthesized before the prepupal stage, by somatic cells outside the ovary or by somatic (follicle) cells of the ovary itself.

show abstract

Production of male and female offspring in the strictly monogenic fly Chtysomya rufifacies after ovary transplantation

Cited by 4 publications

References 5 publications

The Incidences. Mechanisms and Evolution of Cytoplasmic Sex Ratio Distorters in Animals

The Incidences. Mechanisms and Evolution of Cytoplasmic Sex Ratio Distorters in Animals

Sexual Dimorphism in Growth Rate and Gene Expression Throughout Immature Development in Wild Type Chrysomya rufifacies (Diptera: Calliphoridae) Macquart

Analysis of the predetermining effect of a sex realizer by ovary transplantations in the monogenic flyChrysomya rufifacies

Contact Info

Product

Resources

About