Production of Mare Chorionic Girdle Organoids That Secrete Equine Chorionic Gonadotropin

Thompson, Riley E.; Meyers, Mindy A.; Palmer, Jennifer S.; Veeramachaneni, D. N. Rao; Magee, Christianne; Mestre, Amanda M. de; Antczak, Douglas F.; Hollinshead, Fiona K.

doi:10.3390/ijms24119538

Cited by 3 publications

(2 citation statements)

References 42 publications

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“…Механізм дії ФСГподібної активності eCG повністю не з'ясований, вважається, що він зумовлений структурною схожістю між eCG та ФСГ (Murphy, 2012). Оскільки eCG може мати як ФСГ-, так і ЛГ-подібну активність, його можна використовувати для індукції як тічки, так і овуляції залежно від виду та дози, а для отримання eCG у кобил збирають великі об'єми цільної крові один або два рази на тиждень (Vilanova et al, 2019;Thompson et al, 2023).…”

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“…До того ж для підтримання вагітності у кобили, введення eCG має важливе значення для багатьох програм розведення домашніх і недомашніх тварин, особливо тих, що утримуються в неволі (Lucia et al, 1999;Liu et al, 2007;Thompson et al, 2023). В інших видів, крім коней, eCG індукує вивільнення як ЛГ, так і ФСГ (Crowe & Mullen, 2013).…”

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Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

Tverdokhlib

2023

SMLNUVMB

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Ovulatory stimulation of rabbits during artificial insemination requires the use of hormonal drugs to stimulate maturation of follicles, effective fertilization and full manifestation of female sexual function. For this purpose, gonadotropins or analogues of gonadotropin-releasing hormone are common in the practice of rabbit breeding. The latter have a selective effect on folliculogenesis and, under certain conditions, are not capable of inducing ovulation in rabbits of “zero” reproductive cycle, that is, those that have not given birth. Instead, gonadotropins, in particular serum gonadotropin obtained from foal mares (eCG), have a pronounced effect on folliculogenesis in animals. eCG is a glycoprotein hormone secreted by trophoblastic epithelial cells of fetal origin. In other species, except for horses, it is able to induce the release of both luteinizing and follicle-stimulating hormone. That is why eCG is widely used together with human chorionic gonadotropin to induce follicle growth and ovulation. However, there are data on complications and pathological conditions that may occur with long-term use of eCG, especially in genital tract. Therefore, the goal of our research was the morphological evaluation of the functional compartments of ovaries when using eCG to stimulate reproductive function of rabbits. Schemes of hormonal treatment of females in the conditions of a private rabbit farm, an experiment was conducted, which included the introduction of eCG in different doses (40 IU for animals of experimental group 1 and 25 IU for experimental group 2) during five reproductive cycles. Selection of material for histological studies, its fixation, production and analysis of tissue sections stained with hematoxylin and eosin were performed according to commonly used methods. The complex of morphofunctional changes in the ovaries of the rabbits of experimental groups corresponds to the pathomorphological picture of the syndrome of hyperstimulated ovaries. In our experiment, obtained changes were more pronounced at a dose of 40 IU, using which we additionally detected signs of increased immunoreactivity in body of rabbits, as evidenced by the presence of nodular lymphoid formations in outer theca of secondary and tertiary follicles. Thus, as a safe regimen for the use of eCG for ovulatory stimulation in rabbits, we recommend a dosage of 25 IU for 1–2 reproductive cycles.

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Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

Tverdokhlib

2023

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Gross and ultrasonic morphology of the equine conceptus on days 10 to 40

Gille,

Ginther

2023

Journal of Equine Veterinary Science

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Histomorphology of the ovaries of rabbits does during ovulation induced by the combined use of gonadotropins

Tverdokhlib,

Naumenko,

Koshevoy

et al. 2024

Ukr. Jour. of Vet. and Agr. Sci.

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Hormonal stimulation of folliculogenesis is a general method of increasing reproductive capacity in rabbits; however, studying the safety of the developed protocols remains an urgent scientific task. The use of hormonal agents is due to the physiological characteristics of rabbits, primarily coitus-dependent mechanisms of ovulation. Applying gonadotropin-releasing analogs depends on mature follicles ready for ovulation in the ovary. It, therefore, has limited effectiveness for rabbits of the “zero” cycle that have not yet given birth. Therefore, such females are recommended to use medications based on gonadotropins obtained from the serum of foal mares (eCG) or human chorionic gonadotropin (hCG). There is limited data on the combined use of gonadotropins, especially the long-term administration in different doses. Thus, the research aimed to conduct a morphological evaluation of rabbit's ovaries with combined serum and chorionic gonadotropins to stimulate folliculogenesis before artificial insemination to assess the safety of the proposed dosages and long-term administration. Stimulation of ovulation in rabbits of experimental groups was caused by the combined use of serum and chorionic gonadotropins (eCG – 400 IU; hCG – 200 IU) for experimental groups 1 and 2 at a dose of 40 IU and 24 IU, respectively. Females of the control group were induced to ovulate by subcutaneous injection of 0.2 ml of gonadotropin-releasing hormone analog. Ovaries were collected on the seventh day of gestation after the euthanasia of the rabbit with prior premedication. After dissection and macroscopic examination, the ovaries were fixed in a 10% aqueous solution of neutral formalin. Histological slides were made according to the general method by pouring in paraffin and subsequent staining with hematoxylin and eosin. The research has established that repeated (during five reproductive cycles) combined use of serum and chorionic gonadotropins has dose-dependent effectiveness and can cause dysfunction of ovaries and blood circulation. Pathological changes may acquire a systemic character with the manifestation of critical conditions for the health and life of rabbits. The stimulating effect of the combined use of eCG and hCG was detected at a dose of 40 IU, and the inhibition of folliculogenesis was observed at 24 IU. Since the premises for the hyperandrogenism were found microscopically, it was essential to investigate the level of androgens in the blood of rabbits after using a dose of 24 IU. At the same time, both doses of gonadotropins affect the reactivity of the endothelium, which is manifested by the presence of intravascular vesicles and changes in the rheological properties of blood.

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Production of Mare Chorionic Girdle Organoids That Secrete Equine Chorionic Gonadotropin

Cited by 3 publications

References 42 publications

Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

Morphological assessment of ovaries during stimulation of ovulation in rab-bits with equine serum gonadotropin in different doses

Gross and ultrasonic morphology of the equine conceptus on days 10 to 40

Histomorphology of the ovaries of rabbits does during ovulation induced by the combined use of gonadotropins

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