Production of Menaquinone(Vitamin K2)-7 by Bacillus subtilis.

Abstract

Menaquinone-7 (MK-7) is a highly bioactive homologue of vitamin K. We obtained a diphenylamine-resistant mutant strain D200-41 from Bacillus subtilis strain MH-1 which was isolated from fermented soybeans, natto. The mutant strain exhibited decreased production of MK-6. Using strain D200-41, efficient production of MK-7 was achieved. We found that, compared with an agitated and aerated culture, production of MK-7 was increased by static culture. The sporulation of the cells progressed more slowly in a static culture than in an agitated culture. The maximum concentration of MK reached about 60 mg/l in a medium containing 10% soybean extract, 5% glycerol, 0.5% yeast extract and 0.05% K2HPO4 (pH 7.3) when D200-41 cells as well as MH-1 cells were statically cultured at 45 degrees C for 5 d after being cultured with shaking at 37 degrees C for 1 d.

Menaquinones, Bacteria, and Foods: Vitamin K2 in the Diet

“…The highest measured value is almost 1000 μg/100 g. B. subtilis natto is the key microorganism for industrial production of MK-7, and much work has been done to improve the production. Optimization of the fermentation medium, mutations of the strains, and biofilm formation have been described as means for improving the yield of MK-7 [42][43][44][45][46]. The use of organic solvents to extract vitamins is one of the major issues of the bulk production of MK-7.…”

Vitamin K2 Rich Food Products

“…Commercially, MK-7 was produced by action of bacteria such as E. coli, Flavobacterium, and B. subtilis natto. Moreover, among the fermented microflora, B. subtilis is consider one of the best options for the production of MK-7 due to its safety aspects as well as potential to synthesis the range of menaquinones such as MK-4 to MK-8 [15,24,29]. These hydrolytic bacteria are significantly consumed and reduced the indigestible oligo-and polysaccharides.…”

Exometabolites Phytostimulation Activity of Antagonistic Active Strain Bacillus Amyloliquefaciens Imv В-7404

“…amyloliq-amyloliquefaciens IÌВ В-7404. Öе дозволяє зробити припущення щодо наявності інших регуляторів негорìональної природи у культуральній рідині дослі-джуваного штаìу, зокреìа, вітаìіну Ê, синтез якого є характерниì для сапробіонтів роду Bacillus [13].…”