Production of metabolites in the biodegradation of phenanthrene, fluoranthene and pyrene by the mixed culture of Mycobacterium sp. and Sphingomonas sp.

Zhong, Yin; Luan, Tiangang; Lin, Li; Liu, Hong; Tam, N.F.Y.

doi:10.1016/j.biortech.2010.09.113

Cited by 104 publications

(56 citation statements)

References 35 publications

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“…Although dihydroxypyrene standard was not commercially available, three peaks at the retention time of 24.26, 27.39, and 27.75 min were determined as dihydroxypyrene according to ion fragment pattern in mass spectra (378 Figure S1 (Supporting Information), which were labeled as dihydroxypyrene I, II, and III, respectively. 19 The occurrences of pyrene metabolites produced by the algal-only and bacterial-only cultures, as well as the algal−bacterial consortium, are tabulated in Table S1 (Supporting Information). Dihydroxypyrene (I, II, and III) and 1-hydroxypyrene were considered as the initial intermediates in the degradation pathways of pyrene.…”

Section: ■ Results and Discussionmentioning

confidence: 99%

Pyrene Degradation Accelerated by Constructed Consortium of Bacterium and Microalga: Effects of Degradation Products on the Microalgal Growth

Luo

Chen

Lin

et al. 2014

Environ. Sci. Technol.

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Abundant microbes including bacteria, fungi, or algae are capable of biodegrading polycyclic hydrocarbons (PAHs). However, pure cultures never occur in the contaminated environments. This study aimed to understand the general potential mechanisms of interactions between microbes under pollution stress by constructing a consortium of PAH-degrading microalga (Selenastrum capricornutum) and bacterium (Mycobacterium sp. strain A1-PYR). Bacteria alone could grow on the pyrene, whereas the growth of algae alone was substantially inhibited by the pyrene of 10 mg L(-1). In the mixing culture of algae and bacteria, the growth rate of algae was significantly increased from day 4 onward. Rapid bacterial degradation of pyrene might mitigate the toxicity of pyrene to algae. Phenolic acids, the bacterial degradation products of pyrene, could serve as the phytohormone for promoting algal growth in the coculture of algae and bacteria. In turn, bacterial growth was also enhanced by the algae presented in the mixing culture. Consequently, the fastest degradation of pyrene among all biodegradation systems was achieved by the consortium of algae and bacteria probably due to such interactions between the two species by virtue of degradation products. This study reveals that the consortium containing multiple microbial species is high potential for microbial remediation of pyrene-contaminated environments, and provides a new strategy to degrade the recalcitrant PAHs.

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Section: ■ Results and Discussionmentioning

confidence: 99%

Pyrene Degradation Accelerated by Constructed Consortium of Bacterium and Microalga: Effects of Degradation Products on the Microalgal Growth

Luo

Chen

Lin

et al. 2014

Environ. Sci. Technol.

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“…S. koreensis was tested to grow on phthalate as a sole source of energy on MBS agar medium; the heavy growth was obtained after 24 hours. Zhong et al [41] detected only two metabolites (monohydroxypyrene and pyrene diol) from pyrene degradation by mixed culture of Sphingomonas and Mycobacterium, while, when Sphingomonas was used as pure culture for pyrene degradation, no metabolites could be detected.…”

Section: Discussionmentioning

confidence: 99%

Biodegradation Ability and Catabolic Genes of Petroleum-DegradingSphingomonas koreensisStrain ASU-06 Isolated from Egyptian Oily Soil

Hesham

Mawad

Mostafa

et al. 2014

BioMed Research International

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Polycyclic aromatic hydrocarbons (PAHs) are serious pollutants and health hazards. In this study, 15 PAHs-degrading bacteria were isolated from Egyptian oily soil. Among them, one Gram-negative strain (ASU-06) was selected and biodegradation ability and initial catabolic genes of petroleum compounds were investigated. Comparison of 16S rRNA gene sequence of strain ASU-06 to published sequences in GenBank database as well as phylogenetic analysis identified ASU-06 as Sphingomonas koreensis. Strain ASU-06 degraded 100, 99, 98, and 92.7% of 100 mg/L naphthalene, phenanthrene, anthracene, and pyrene within 15 days, respectively. When these PAHs present in a mixed form, the enhancement phenomenon appeared, particularly in the degradation of pyrene, whereas the degradation rate was 98.6% within the period. This is the first report showing the degradation of different PAHs by this species. PCR experiments with specific primers for catabolic genes alkB, alkB1, nahAc, C12O, and C23O suggested that ASU-06 might possess genes for aliphatic and PAHs degradation, while PAH-RHDαGP gene was not detected. Production of biosurfactants and increasing cell-surface hydrophobicity were investigated. GC/MS analysis of intermediate metabolites of studied PAHs concluded that this strain utilized these compounds via two main pathways, and phthalate was the major constant product that appeared in each day of the degradation period.

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“…Pyrene (a four-ring PAHs) is one of the most abundant high-molecular weight PAHs present in the environment and due to its low bioavailability recalcitrant property, removal from the environment is a challenging task. Degradation of pyrene or other PAHs occurs mostly by microbial means and many bacterial species have been involved in this process (Zhong et al 2011;Kumari et al 2013;Ma et al 2013;Ghosh et al 2014).…”

Section: Introductionmentioning

confidence: 99%

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Singh

Tiwary

2016

Bioresour. Bioprocess.

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Background: Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural characterization of the BS produced by Pseudomonas otitidis P4.Results: An efficient BS producing bacterial strain isolated from the unexplored coal mining site of Chirimiri, India was identified as P. otitidis P4 based on morphological, biochemical and 16S rRNA gene sequence analysis. The surface tension of the culture medium was reduced from 71.18 to 33.4 mN/m. The surface tension and emulsification activity of the BS remained stable over a wide range of temperature, pH and salt concentrations indicating its scope of application in bioremediation, food, cosmetics, and pharmaceutical industries. Structural attributes of BS were determined by biochemical tests, thin layer chromatography (TLC), Fourier transform infrared (FTIR) and nuclear magnetic resonance ( 1 H NMR) spectroscopy analyses, which confirmed the glycolipid nature of BS. Lipid and sugar fractions were the main constituents of the extracted BS. Thermogravimetric (TG) and Differential scanning calorimetry (DSC) analyses showed the thermostable nature of BS. As determined from TGA graph, the degradation temperature of biosurfactant was found to be 280 °C while complete weight loss was observed after 450 °C. Conclusion:The BS isolated from P. otitidis P4 was identified as glycolipid and showed high emulsification activity and stability in a wide range of temperature, pH and salinity which makes it suitable for various industrial and environmental applications.

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Production of metabolites in the biodegradation of phenanthrene, fluoranthene and pyrene by the mixed culture of Mycobacterium sp. and Sphingomonas sp.

Cited by 104 publications

References 35 publications

Pyrene Degradation Accelerated by Constructed Consortium of Bacterium and Microalga: Effects of Degradation Products on the Microalgal Growth

Pyrene Degradation Accelerated by Constructed Consortium of Bacterium and Microalga: Effects of Degradation Products on the Microalgal Growth

Biodegradation Ability and Catabolic Genes of Petroleum-DegradingSphingomonas koreensisStrain ASU-06 Isolated from Egyptian Oily Soil

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

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