Production of Methane, Hydrogen and Ethanol from Secale cereale L. Straw Pretreated with Sulfuric Acid

Domański, Jarosław; Marchut-Mikołajczyk, Olga; Cieciura-Włoch, Weronika; Patelski, Piotr; Dziekońska‐Kubczak, Urszula; Januszewicz, Bartłomiej; Zhang, Bolin; Dziugan, Piotr

doi:10.3390/molecules25041013

Cited by 14 publications

(3 citation statements)

References 46 publications

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“…Figure . 11, illustrated that in collagen concentration from 10 g VSS/L to 20 g VSS/L until the fth-day methane production did not occur with increasing pH. Therefore in this time range, there was classical dark fermentation [42].…”

Section: Hydrogen Sulphide Emissionmentioning

confidence: 92%

Microbial Biogas Production From Pork Gelatine.

Sołowski

2021

Preprint

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Dark fermentation of collagen (gelatine) results are shown in this research. The concentrations of applied gelatine were of VSS (volatile suspended solids) from 10 g VSS/L to 30 g VSS/L. The initial process pH was 5.5, depending on concentration reached pH values from 7.5 to 7.8 after 55 days. Although inoculum was heat-shocked in the case of 30 g VSS/L of collagen the process was hydrogenotrophic anaerobic digestion. In collagen concentration below 30 g VSS/L, hydrogen production was dominant only in the first 5 days of experiments. Then there also changed from dark fermentation into hydrogenotrophic methane production. In the case of optimal biogas production was due to accumulative production for a concentration of collagen 20 g VSS/L: 147.2 mL of hydrogen and 57.23 L of methane. In the case of optimal biogas production was due to accumulative production for a concentration of collagen 20 g VSS/L: 147.2 mL of hydrogen and 57.23 L of methane. The optimal hydrogen and methane yields were for concentration 10 g VSS/L (7.65 mL H2 /g VSS, and 3.49 L CH4/ g VSS). In 10 g VSS/L was also the lowest accumulated emission of hydrogen sulphide (10.3 mL of H2S), while the lowest yield was for 30 g VSS/L (0.44 mL H2S /g VSS). After a lag time, the hydrogen production and hydrogen sulphide grew with a specific ratio depending on concentration. Collagen, a protein with known amounts of sulphur allowed determining the origin of hydrogen sulphide in biogas. The hydrogen sulphide emission and sulphur added analysis proved that hydrogen sulphide origins in biogas from bacteria remains more than from substrate.

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Section: Hydrogen Sulphide Emissionmentioning

confidence: 92%

Microbial Biogas Production From Pork Gelatine.

Sołowski

2021

Preprint

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“…Dahunsi et al (2019) also stated that sulfuric acid pretreatment significantly reduced hemicellulose and partial cellulose solubilization. Furthermore, Domański et al (2020) investigated that methane yield increased with increasing sulfuric acid concentrations during methane production of rye straw. Therefore, this study chose sulfuric acid pretreatment as chemical pretreatment for corn stover.…”

Section: Introductionmentioning

confidence: 99%

Anaerobic Digestion of Corn Stover Pretreated with Sulfuric Acid in Different Soaking Durations

2023

Global NEST: The International Journal

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<p>The biogas production of pretreated corn stover has been determined in different soaking durations. Batch anaerobic digestion applies three different soaking durations in sulfuric acid pretreatment under room temperature. The study aimed to probe the effect of soaking durations during sulfuric acid pretreatment. The experiment was conducted in 600 mL digesters at room temperature. Biogas volume was measured using the water displacement method every three days. The observed cumulative biogas yields varied between 48.74 mL/g VS and 99.95 mL/g VS. The highest biogas yield was obtained when corn stover was soaked in sulfuric acid for 6 hours. The 24 h-pretreated corn stover got the lowest biogas yield. The statistical result proved a significant effect of soaking durations on biogas production (p < 0.05). The logistic model provided a better fit than the first-order model, with R2 values ranging from 0.9923 to 0.9987 and divergence between experimental and predicted values varying between 0.12% and 1.48%.</p>

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“…Rye ( Secale cereale L.) is an allogamous, diploid (RR, 2 n = 2x = 14), and widely grown cereal crop in northern Europe [ 22 ]. In recent years, it has also been used for substrate for bioethanol and biogas production [ 23 , 24 ]. Rye is reported to have a large genome of approximately 7.9 Gb [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

A Comparative Transcriptome Analysis, Conserved Regulatory Elements and Associated Transcription Factors Related to Accumulation of Fusariotoxins in Grain of Rye (Secale cereale L.) Hybrids

Mahmood

Orabi²,

Kristensen³

et al. 2020

IJMS

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Detoxification of fusariotoxin is a type V Fusarium head blight (FHB) resistance and is considered a component of type II resistance, which is related to the spread of infection within spikes. Understanding this type of resistance is vital for FHB resistance, but to date, nothing is known about candidate genes that confer this resistance in rye due to scarce genomic resources. In this study, we generated a transcriptomic resource. The molecular response was mined through a comprehensive transcriptomic analysis of two rye hybrids differing in the build-up of fusariotoxin contents in grain upon pathogen infection. Gene mining identified candidate genes and pathways contributing to the detoxification of fusariotoxins in rye. Moreover, we found cis regulatory elements in the promoters of identified genes and linked them to transcription factors. In the fusariotoxin analysis, we found that grain from the Nordic seed rye hybrid “Helltop” accumulated 4 times higher concentrations of deoxynivalenol (DON), 9 times higher nivalenol (NIV), and 28 times higher of zearalenone (ZEN) than that of the hybrid “DH372” after artificial inoculation under field conditions. In the transcriptome analysis, we identified 6675 and 5151 differentially expressed genes (DEGs) in DH372 and Helltop, respectively, compared to non-inoculated control plants. A Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that DEGs were associated with glycolysis and the mechanistic target of rapamycin (mTOR) signaling pathway in Helltop, whereas carbon fixation in photosynthesis organisms were represented in DH372. The gene ontology (GO) enrichment and gene set enrichment analysis (GSEA) of DEGs lead to identification of the metabolic and biosynthetic processes of peptides and amides in DH372, whereas photosynthesis, negative regulation of catalytic activity, and protein-chromophore linkage were the significant pathways in Helltop. In the process of gene mining, we found four genes that were known to be involved in FHB resistance in wheat and that were differentially expressed after infection only in DH372 but not in Helltop. Based on our results, we assume that DH372 employed a specific response to pathogen infection that led to detoxification of fusariotoxin and prevented their accumulation in grain. Our results indicate that DH372 might resist the accumulation of fusariotoxin through activation of the glycolysis and drug metabolism via cytochrome P450. The identified genes in DH372 might be regulated by the WRKY family transcription factors as associated cis regulatory elements found in the in silico analysis. The results of this study will help rye breeders to develop strategies against type V FHB.

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Production of Methane, Hydrogen and Ethanol from Secale cereale L. Straw Pretreated with Sulfuric Acid

Cited by 14 publications

References 46 publications

Microbial Biogas Production From Pork Gelatine.

Microbial Biogas Production From Pork Gelatine.

Anaerobic Digestion of Corn Stover Pretreated with Sulfuric Acid in Different Soaking Durations

A Comparative Transcriptome Analysis, Conserved Regulatory Elements and Associated Transcription Factors Related to Accumulation of Fusariotoxins in Grain of Rye (Secale cereale L.) Hybrids

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