Production of Microbial Biomass Protein from Potato Processing Wastes by Cephalosporium eichhorniae

Abstract

The use of Cephalosporium eichhorniae 152 (ATCC 38255) (reclassified as Acremonium alabamense; see Addendum in Proof), a thermophilic, acidophilic, amylolytic fungus, for the conversion of potato processing wastes into microbial protein for use as animal feed was studied. The fungus was not inhibited by a-solanine or 0-2-chaconine, antimicrobial compounds in potatoes, or by morpholine or cyclohexylamine (additives to steam used in the peeling process) at levels likely to be encountered in this substrate. Mixed effluent from holding tanks at a potato-processing plant contained about 109 bacteria per ml and inhibited fungal growth. * Corresponding author. eichhorniae 152 for the production of MBP from potato processing wastes. MATERIALS AND METHODS Culture. C. eichhorniae (Padwick) 152 was isolated by Gregory et al. (8) from soil. It was grown on potato dextrose agar (Difco Laboratories) at 45°C and stored at room temperature.

Converting corn wet‐milling effluent into high‐value fungal biomass in a biofilm reactor

“…These findings agree with nutrient supplementation studies conducted on other food-processing effluents. Stevens and Gregory (1987) reported efficient growth of Cephalosporium eichhorniae with N supplementation as (NH 4 ) 2 SO 4 in potato processing effluent. Truong et al (2004) also observed better growth of A. oryzae yield with higher COD removal in cassava starch processing effluent supplemented with (NH 4 ) 2 SO 4 or peptone.…”

“…On the other hand, fungi are often cultivated commercially as a source of high-value byproducts under aseptic conditions on relatively expensive substrates such as starch or molasses (Barbesgaard et al, 1992). The use of molds (microfungi) is an attractive approach since the fungal process converts the organics into high-value fungal biomass that can be used as a source of protein in animal feed and potentially in human diets (Stevens and Gregory, 1987). Fungi produce a wide range of biochemicals and enzymes, and are usually more effective in metabolizing complex carbohydrates such as starch than bacteria (Van Leeuwen et al, 2003).…”

Enhancing dry-grind corn ethanol production with fungal cultivation and ozonation

“…The filamentous nature of fungal mycelia and potential for pellet formation, however, simplify separation of the biomass from the liquid substrate Nigam, 1994). Unlike bacterial cells, the highly-dewaterable fungal biomass has commercial value as a protein source suitable for animal feed and even for human food, depending on the substrate and processing steps (Stevens and Gregory, 1987).…”

“…Diverse fungal species and substrates, such as wastewaters, have been evaluated in lab-scale, batch studies to produce fungal biomass for animal feed (Hiremath et al, 1985;Thanh and Simard, 1973a, b). As discussed previously, fungal wastewater treatment is a low-cost, technically-simple option for producing this alternative protein source (Stevens and Gregory, 1987).…”

“…Actinomucor elegans Hang, 1976 Aspergillus fumigatus Khor et al, 1977;Reade and Gregory, 1975 Aspergillus niger Christias et al, 1975;Hang, 1976;Oboh et al, 2002;Singh et al, 1991 Aspergillus oryzae Hang, 1976 Candida lipolytica Achermowicz et al, 1977 Candida tropicalis Achermowicz et al, 1977;Christias et al, 1975 Candida utilis Villas-Boas et al, 2003 Chaetomium globosum Hang, 1976 Fusarium moniliforme Christias et al, 1975;Drouliscos et al, 1976 Fusarium oxysporum Sukara and Doelle, 1989 Fusarium venenatum Anderson and Solomons, 1984;Trinci, 1992 Geotrichum candidum Robinson and Smith, 1984;Ziino et al, 1999 Pestalotriopsis westerdijkii Hang, 1976 Phanerochaete chrysosporium Cardoso and Nicoli, 1981 Rhizopus oligosporus Sukara and Doelle, 1989 Thielavia terrestris Bajon et al, 1985;Stevens and Gregory, 1987 Trichoerma viride Hang, 1976;Youssef and Aziz, 1999 a References collected by Thrane (2007).…”