Production of Microshoot Using the Temporary Immersion System (Tis) in Potato Cultivars

Abstract

The Temporary Immersion System (TIS) Bioreactor’s performance in cultivation for several commercial potato cultivars became the prevailing study’s review focus. The experiment in a split-plot design had two factors. The first factor comprised the type of culture system (conventional and TIS Bioreactor) treatment used as main plots. The second factor was the four potato cultivars (Atlantic Malang, Dayang Sumbi, Granola L., and Maglia) used as subplots. The TIS Bioreactor culture has the highest average in all studied variables compared with conventional cultures at the multiplication stage. In the TIS Bioreactor, potato cultivar Dayang Sumbi excelled in the number of axillary branching and number of primary roots. At the same time, genotype Granola L. surpassed the height of the plantlets of other cultivars. Meanwhile, the cultivar Atlantic Malang shone in the number of nodes, with Maglia in the number of leaves. Using the TIS Bioreactor produces microshoots with more nodes and can be beneficial as a propagation organ. The Dayang Sumbi cultivar was superior to all other potato cultivars.