Production of Monoclonal Antibodies Against Three Ilarviruses and Alfalfa Mosaic Virus and Their Use in Serotyping

Halk, Edward L.; Hsu, H. T.; Aebig, Joan; Franke, J.

doi:10.1094/phyto-74-367

Cited by 53 publications

(34 citation statements)

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“…Monoclonal antibodies (M-Ab) produced by somatic cell hybridization (K6hler & Milstein, 1975) have great potential for plant virus detection (Gugerli & Fries, 1983;Diaco et al, 1984;Hill et al, 1984) as well as serological differentiation (Briand et al, 1982;Diaco et al, 1983;Gugerli & Fries, 1983 ;Halk et al, 1984;Hsu et al, 1984). The objectives of this research were to prepare a highly sensitive, non-isotopic, M-Ab-based immunoassay that could be used to detect SMV antigen in infected seeds.…”

Section: Introductionmentioning

confidence: 99%

Monoclonal Antibody-based Biotin-Avidin ELISA for the Detection of Soybean Mosaic Virus in Soybean Seeds

Diaco¹,

Hill

et al. 1985

Journal of General Virology

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SUMMARYTwo monoclonal antibodies (M-Ab) specific for different epitopes on particles of soybean mosaic virus (SMV) were used in a double-antibody sandwich ELISA (M-Ab ELISA). The non-isotopic immunoassay, which used a biotinylated second antibody and an avidin-alkaline phosphatase detection system to detect SMV in soybean seed extracts, was compared with a polyclonal antibody-based solid-phase radioimmunoassay (SPRIA). M-Ab ELISA detected less than l0 ng of SMV/ml and was more sensitive than the SPRIA which detected 25 ng SMV/ml. Furthermore, M-Ab. ELISA required less than 36 h for seed sample assays and only 5.5 h for assays involving purified virus, whereas SPRIA required 3 or 4 days. When seeds from 33 field plots, in which 0~, 30~ and 50~o of the soybean plants had been inoculated with SMV, were assayed by both systems, results of the two tests correlated for 31 of 33 (94~) seed samples. This suggests that dual-site biotin-avidin M-Ab ELISA systems have potential utility for routine screening of seed samples.

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Section: Introductionmentioning

confidence: 99%

Monoclonal Antibody-based Biotin-Avidin ELISA for the Detection of Soybean Mosaic Virus in Soybean Seeds

Diaco¹,

Hill

et al. 1985

Journal of General Virology

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“…MAbs have been previously produced to AMV (Halk et al, 1984;Halk, 1986) and some of these were shown to recognize epitopes on both intact virus particles and dissociated coat protein subunits. However, others must have been directed to conformation-specific epitopes (neotopes) because they reacted with intact AMV particles but not with coat protein subunits (Halk, 1986).…”

Section: Discussionmentioning

confidence: 99%

Differentiation and Antigenic Characterization of Closely Related Alfalfa Mosaic Virus Strains with Monoclonal Antibodies

Hajimorad

Dietzgen

Francki

1990

Journal of General Virology

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A panel of 15 mouse monoclonal antibodies (MAbs) was raised against five strains of alfalfa mosaic virus (AMV) which were closely related antigenically but biologically distinct. A wide diversity of MAb specificity was revealed by screening them in three formats of indirect ELISA, using native and glutaraldehydefixed AMV particles as well as isolated coat protein preparations. Of these MAbs, seven reacted specifically with only one AMV strain in at least one ELISA format and at least one MAb was capable of identifying each of the strains. One of the MAbs reacted with a cryptotope, whereas the other recognized different subtypes of either metatopes or neotopes, indicating that the AMV particle has a complex antigenic structure. Only two of the MAbs precipitated AMV in agarose gels. Another two, which recognized epitopes on coat protein subunits, also reacted well in immunoblots. One of the precipitating MAbs recognized an epitope which appears to be common to AMV and cucumber mosaic virus.

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“…Leaves which exhibit strong mosaic symptoms in the early season may develop large necrotic areas during period of high temperature or increase sunlight and drop prematurely. In infected plants, symptoms are usually outstanding in spring but tend to fade away as the season progresses and are little or not evident on summer vegetation (Halk et al, 1984;Imed et al, 1997).…”

Section: Symptomatologymentioning

confidence: 99%

Ilarviruses and the Importance of Certified Elite Planting Material in Apple Production System - An Overview

Kapoor¹,

Sharma²,

Shylla³

et al. 2018

Int.J.Curr.Microbiol.App.Sci

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Production of Monoclonal Antibodies Against Three Ilarviruses and Alfalfa Mosaic Virus and Their Use in Serotyping

Cited by 53 publications

References 0 publications

Monoclonal Antibody-based Biotin-Avidin ELISA for the Detection of Soybean Mosaic Virus in Soybean Seeds

Monoclonal Antibody-based Biotin-Avidin ELISA for the Detection of Soybean Mosaic Virus in Soybean Seeds

Differentiation and Antigenic Characterization of Closely Related Alfalfa Mosaic Virus Strains with Monoclonal Antibodies

Ilarviruses and the Importance of Certified Elite Planting Material in Apple Production System - An Overview

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