Production of Monoclonal Antibodies against Common Epitopes of Salmonella by Oral Immunization of Laboratory Animals

Abstract

Materials and Methods: Twelve mice were divided into two groups and immunized orally with S. suberu (3.10: g, m) and S. strasburg from the OD2 group (O:9.46). The Köhler and Milstein method yielded IgA and IgM class IgA secreting monoclonal antibodies. They were characterized by the methods of slide-agglutination, haemagglutination, ELISA, immunoblotting. Results: Four anti-flagelar MAbs of class IgA and one anti-O-class IgM class were obtained. All of the IgA class reacted with the Hg epitope of flags obtained from Salmonella strains and S. enteritidis flagellin. In IgMMAbs, only O-antigens isolated from Salmonella strains belonging to the OD2 group were present. Conclusion:Through this method of oral immunization, besides highly specific IgA MAbs, we also received a clone of hybridomas expressing specific IgM MAbs which by ELISA and SAT show excellent opportunity to participate in diagnostic reactions against salmonellae with a common group epitope OD2.