Production of monoclonal antibodies and enzyme immunoassay to bovine retinol-binding protein and determination of retinol-binding protein serum levels and retinol concentrations in serum and liver in dairy cows before and after parturition

Lindberg, L.‐A.; Sinkkonen, H; Pösö, A.R.; Tesfa, Alem Tsehai; Schröder, Jim

doi:10.1053/rvsc.1998.0280

Cited by 13 publications

(14 citation statements)

References 14 publications

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“…In contrast to present results, Selim et al (2014) reported no time-related differences in RBP4 mRNA expression at −10, 1, and 9 d relative to parturition. Higher protein level and longer close-up feeding with concentrate in our previous study (Selim et al, 2014) than in the present one may explain the discrepancies in the expression patterns of RBP4, as Lindberg et al (1999) observed that higher protein intake before parturition ensured a more stable plasma RBP4 concentration around parturition. In addition to its role as a retinol carrier in plasma, RBP4 may play a role in obesity-induced insulin resistance and regulation of hepatic glucose production, particularly by increasing PCK1 expression (Yang et al 2005).…”

Section: Effect Of Overfeeding On Hepatic Gene Expressioncontrasting

confidence: 52%

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Selim

Kokkonen

Taponen

et al. 2015

Journal of Dairy Science

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Prepartal energy overfeeding may predispose cows to a state of increased insulin resistance with greater lipolysis after parturition. The aim of the study was to evaluate the effects of prepartal overfeeding in terms of abundant grass silage ration on the liver and subcutaneous adipose tissue (SAT) gene expression around parturition. Sixteen multiparous Finnish Ayrshire dairy cows were fed ad libitum either grass silage [high energy, HE; 144 MJ/d of metabolizable energy (ME) intake, n=8] or a mixture of grass silage, wheat straw, and rapeseed meal [55:40:5 (CON), 109 MJ/d of ME, n=8] during the dry period (58.2±4.89 d, mean ± standard deviation). Tissue biopsies and blood samples were collected at -14 (±4.98), 1, and 7 d relative to the actual parturition date. The HE cows had greater total dry matter intake, ME intake, and ME balance during the dry period than the CON cows. Compared with CON, the increases in body weight and body condition score were greater in HE during the dry period. Milk yield during the first 2 wk of lactation was not different between the groups. Plasma glucose, nonesterified fatty acids, insulin, glucagon, and β-hydroxybutyrate did not differ between the groups during the transition period. Dietary treatment did not affect hepatic triglyceride content; however, a delayed increase in hepatic total lipid content was observed in the HE cows at d 1 postpartum. Hepatic cytosolic phosphoenolpyruvate carboxykinase 1 mRNA expression was lower in HE than in CON at d 1 and 7 postpartum. Adiponectin receptor 1 and 2 mRNA abundance tended to be lower in SAT of HE than CON. Lower lipoprotein lipase, leptin, and stearoyl-coenzyme A desaturase mRNA abundances were observed at d 7 postpartum in SAT of the HE cows compared with the CON cows. We concluded that prepartal ad libitum feeding of grass silage may decrease insulin sensitivity and lipogenesis in SAT during peripartal period and may attenuate the increase of hepatic gluconeogenic capacity from propionate compared with a controlled-energy diet.

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Section: Effect Of Overfeeding On Hepatic Gene Expressioncontrasting

confidence: 52%

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Selim

Kokkonen

Taponen

et al. 2015

Journal of Dairy Science

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“…Our findings are in contrast to the findings of Rezamand et al (39), who observed that physiological state affected hepatic RBP4 expression during the periparturient period. Lindberg et al (30) showed that protein supplementation before parturition induces a more stable plasma RBP4 concentration near parturition. Therefore, it is possible that different observations of variation of the plasma RBP4 concentration reflect differences in protein status of cows.…”

Section: Discussionmentioning

confidence: 99%

Prepartal dietary energy alters transcriptional adaptations of the liver and subcutaneous adipose tissue of dairy cows during the transition period

Selim

Salin

Taponen

et al. 2014

Physiological Genomics

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Overfeeding during the dry period may predispose cows to increased insulin resistance (IR) with enhanced postpartum lipolysis. We studied gene expression in the liver and subcutaneous adipose tissue (SAT) of 16 Finnish Ayrshire dairy cows fed either a controlled energy diet [Con, 99 MJ/day metabolizable energy (ME)] during the last 6 wk of the dry period or high-energy diet (High, 141 MJ/day ME) for the first 3 wk and then gradually decreasing energy allowance during 3 wk to 99 MJ/day ME before the expected parturition. Tissue biopsies were collected at -10, 1, and 9 days, and blood samples at -10, 1, and 7 days relative to parturition. Overfed cows had greater dry matter, crude protein, and ME intakes and ME balance before parturition. Daily milk yield, live weight, and body condition score were not different between treatments. The High cows tended to have greater plasma insulin and lower glucagon/insulin ratio compared with Con cows. No differences in circulating glucose, glucagon, nonesterified fatty acids and β-hydroxybutyrate concentrations, and hepatic triglyceride contents were observed between treatments. Overfeeding compared with Con resulted in lower CPT1A and PCK1 and a tendency for lower G6PC and PC expression in the liver. The High group tended to have lower RETN expression in SAT than Con. No other effects of overfeeding on the expression of genes related to IR in SAT were observed. In conclusion, overfeeding energy prepartum may have compromised hepatic gluconeogenic capacity and slightly affected IR in SAT based on gene expression.

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“…Retinol represents the most abundant retinoid in bovine blood (Van Merris et al 2004) and its levels in steers are maintained for at least 145 d even if the animals are fed a diet with no supplemental vitamin A (Gorocica-Buenfil et al 2008). However, plasma retinol and β-carotene levels in heifers and cows decrease at the end of gestation, reach their lowest values around parturition and increase again during the first week of lactation (Johnston & Chew, 1984; Goff & Stabel, 1990; Lindberg et al 1999; Kumagai et al 2000; Kumagai et al 2001; Nonnecke et al 2001; Goff et al 2002; Van Merris et al 2004; Debier et al 2005; Rezamand et al 2007). The decrease in plasma retinol concentration is probably due to the transfer of large amounts of retinol and its derivatives into colostrum (Goff et al 2002), resulting in the accumulation of retinoids in colostrum rather than in milk (Debier et al 2005).…”

mentioning

confidence: 99%

“…It was reported that the mean serum RBP4 and retinol concentrations in cows 4 weeks before calving were 42 μg/ml (~2 μ μ ) and 53 μg/dl (~1·8 μ μ ), respectively. RBP4 levels decreased one week before parturition and recovered to control levels at one week after calving (Lindberg et al 1999) suggesting the possibility that RBP4, like retinol, is transferred from maternal stores to calves through colostrum and milk. However, a similar decrease in plasma RBP4 levels was found only in cows with subclinical intramammary infections, but not in uninfected cows (Rezamand et al 2007), suggesting the decrease might be due to suppression of hepatic RBP4 synthesis during acute-phase reactions, as demonstrated in rats (Rosales et al 1996).…”

mentioning

confidence: 99%

Retinol binding protein 4 in dairy cows: its presence in colostrum and alteration in plasma during fasting, inflammation, and the peripartum period

Eldaim

Kamikawa

Soliman

et al. 2009

Journal of Dairy Research

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Retinol-binding protein 4 (RBP4) is a plasma protein involved in retinol transportation, and recent evidence in rodents suggests that RBP4 is also a metabolic regulator that modifies insulin sensitivity. To assess how RBP4 levels are regulated in ruminants, we determined the RBP4 concentrations in bovine plasma and milk using Western blot analysis. Plasma RBP4 levels in non-pregnant non-lactating (control) cows were around 45 mg/ml, which were sustained during 60-h fasting, but decreased significantly 4 h after lipopolysaccharide (LPS) administration. Basal plasma retinol concentration was around 30 mg/dl, but this decreased to approximately one-third and one-half of these values during fasting and 8 h after LPS challenge, respectively. Plasma RBP4 and retinol levels in cows 3-6 d before parturition were comparable to those of the controls. However, on the day of parturition both were significantly decreased and had returned to basal levels by two weeks after calving. Interestingly, RBP4 was clearly detected in colostrum (16 . 4±5 . 6 mg/ml) but was only faintly detected in milk from cows at 7 d and 15 d after calving. Retinol concentrations in colostrum were almost 10-fold higher than those in plasma, while those in milk were comparable to those in plasma. These results suggest that RBP4 and retinol levels are independently regulated under physiological and pathophysiological conditions and that RBP4, like retinol, is transferred from maternal stores to calves through colostrum.

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Production of monoclonal antibodies and enzyme immunoassay to bovine retinol-binding protein and determination of retinol-binding protein serum levels and retinol concentrations in serum and liver in dairy cows before and after parturition

Cited by 13 publications

References 14 publications

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Effect of prepartal ad libitum feeding of grass silage on transcriptional adaptations of the liver and subcutaneous adipose tissue in dairy cows during the periparturient period

Prepartal dietary energy alters transcriptional adaptations of the liver and subcutaneous adipose tissue of dairy cows during the transition period

Retinol binding protein 4 in dairy cows: its presence in colostrum and alteration in plasma during fasting, inflammation, and the peripartum period

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