Production of Monoclonal Antibodies (mAcs) Purified Anti-metalloprotease from the Venom of the Serpent <em>Bitis arietans</em>

Abstract

The African snake Bitis arietans is of great medical importance and is found in sub-Saharan Africa and in savannas and pastures of Morocco and western Arabia. Contributes complacently in the epidemiology of snakebites in humans and animals. The lack of specific antivenoms aggravates this situation. Identifying toxins, knowing their toxic properties and developing antitoxins are the goals of emerging projects. This project aims at monoclonal anti-metalloprotease (mAbs) from the toxin of Bitis arietans venom. mAbs will serve as sources of complementarity determining regions (CDR). The main applied methodologies will not develop the project fit mainly in the purification of metalloprotease and immunization of mice to obtain lymphocytes and replicate it. So far, we have been able to highlight a metalloprotease of interest, which will be properly identified for the production of antibodies, a finding that was confirmed by proteomic and transcriptomic analyzes. The next step will be to immunize mice and validate the antibodies produced.