Production of monoclonal antibody to subtype 9 of Neisseria meningitidis and the distribution of this subtype in Brazil

Abstract

A new monoclonal antibody (5F81A4P1.9), which is specific for subtype 9 antigen of meningococci, was studied. The antibodies were raised against a previously non-typable (NT) serogroup B strain from Brazilian patients and were found to react with the subtype antigen of prototype reference strains for subtype 9 (M982), as well as with those of homologous strains. The subtype 9 epitope was found in 6.8% of serogroup B strains among 602 strains of Neisseria meningitidis case isolates, including representative isolates from Brazilian states. Subtype P1.9 was predominantly related to serogroup B in Brazil among the isolates collected during the N. meningitidis epidemic in 1992. No significant differences were observed in the occurrence of subtype P1.9 among strains isolated from several Brazilian states. Fluorescence-activated cell-sorter analysis showed that 5F81A4 MAb recognized a 46 kDa protein on the surface of a homologous strain of N. meningitidis (B:4:P1.9). These results, in association with a bactericidal activity assay for 5F81A4, and with experimental passive protection in mice, demonstrated the importance of subtype 9 class 1 proteins of N meningitidis in Brazil. Serotyping is essential for the development of vaccination strategies.

Production and characterization of monoclonal antibodies specific for Pseudorabies virus

“…In the past period one of the most frequently used serological methods was ELISA (Eloit et al, 1989;Qvist et al, 1990). This assay, however, is not reliable in all cases, so it is necessary to check the results by other methods such as dot ELISA and Western blot (Yoshida and Tsuda, 1998;Abolhassani, 2000;Belo et al, 2004;Sulimenko and Draber, 2004).…”

Prime-Boost Immunogenicity of Class 5C Protein from Neisseria meningitidis in Mice with Different Adjuvants

“…The murine monoclonal antibodies (mAb) used for isolation and characterization of the meningococcal variant against Opc were produced in our laboratory as described previously [45] with some modifications [46]. BALB/c female mice (18-22 g -provided by the animal house of Adolfo Lutz Institute) were injected in the footpad with an emulsion of complete Freund's adjuvant containing 5 g of the purified Opc protein.…”

Utilização de anticorpos monoclonais como diferenciais de meningites bacterianas e virais pela técnica de Imuno-histoquímica.

“…Com aplicabilidade na área de Neisseria meningitidis, o sistema de sorotipagem para meningococos baseia-se em uma variedade de anticorpos monoclonais que reconhecem diferenças antigênicas nas proteínas de membrana externa de classe 2 ou 3 (porina B) e de classe 1 (porina A) . O sistema de tipagem com anticorpos monoclonais foram desenvolvidos por causa das dificuldades encontradas com o uso de soros policlonais hiperimunes para tipagem, como descrito em trabalhos publicados pelo nosso laboratório (BELO, 2004(BELO, , 2007CHAVES et al, 2015;DE GASPARI, ZOLLINGER, 2001;FERRAZ et al, 2008). (POOLMAN et al, 1994).…”

“…meningitidis com o painel de anticorpos monoclonais pré-estabelecido e produção de novos monoclonais para a caracterização de perfis desconhecidos (BELO, 2004(BELO, , 2007DE GASPARI, ZOLLINGER, 2001).…”