Production of multiple cytokines by Hodgkin's disease derived cell lines

Abstract

The production of cytokines was analysed in Hodgkin's disease (HD) derived cell lines by enzyme linked immunosorbent tests (ELISA) and Northern blot experiments. Our results demonstrate that HD derived cell lines produce a variety of cytokines, such as IL1 alpha, IL4, IL5, IL6, IL8, TNF alpha, TNF beta and GM-CSF but not IL1 beta, IL2, IL3 and G-CSF. In cell lines with a high expression of CD25 (the light chain of the IL2 receptor), we found soluble IL2 receptors in the supernatants. In addition, receptors for IL6 could be detected in most of the HD derived cell lines. However the growth of HD derived cell lines, which produce IL6 and IL6 receptors could not be inhibited by anti-IL6 antibodies. From our data we conclude, that IL6 and additional cytokines may be involved in the biology of HD.

STAT3 is constitutively activated in Hodgkin cell lines

“…4,37 The results of immunofluorescence staining for gp80 are in line with our previous reports showing the expression on all analyzed cell lines with the exception of KMH2. 3,4 The recently established HD cell line L1236 also expressed gp80, but at a low level ( Figure 4B). The results of Western blot and flow cytometry analyses are summarized in Table 1, which also provides IL-6 levels detected by ELISA.…”

“…We and other groups have previously shown that IL-6 and IL-6R gp80 are expressed both in HD cell lines and in primary H-RS cells and IL-6, and perhaps additional cytokines may be involved in the pathogenesis of HD. 1,[3][4][5][6] The aim of this study was to evaluate whether IL-6 signal transduction is important for the growth of HD cell lines. Using a panel of well-characterized HD cell lines we identified constitutively activated DNA-binding proteins in these cells.…”

“…by guest www.bloodjournal.org From produce IL-6 and express the IL-6R gp80, could not be inhibited by ␣-IL-6 antibodies and further suggests that cytokines sharing the gp130 receptor subunit are not involved in proliferation of Hodgkin cell lines. 4 The tyrphostin AG490 inhibits STAT3 activation and growth of the Hodgkin cell line L428…”

“…1,2 This histologic pattern of HD may be due to the production and release of cytokines from H-RS cells that are involved in the growth of the lymphoma cells and the interactions with bystander cells. [3][4][5] Recently it has been shown that HD cell lines and primary H-RS cells express interleukin-6 (IL-6) and the ␣-chain of the IL-6 receptor (IL-6R; gp80). 3,4,6 IL-6 is synthesized by a variety of cells on stimulation and acts on a wide range of different target cells to regulate cell growth, differentiation, or gene expression.…”

“…[3][4][5] Recently it has been shown that HD cell lines and primary H-RS cells express interleukin-6 (IL-6) and the ␣-chain of the IL-6 receptor (IL-6R; gp80). 3,4,6 IL-6 is synthesized by a variety of cells on stimulation and acts on a wide range of different target cells to regulate cell growth, differentiation, or gene expression. 7,8 IL-6 may act as an autocrine or paracrine growth factor for multiple myeloma, Epstein Barr virus (EBV)-immortalized B cells, and perhaps for H-RS cells, although this has not been proven yet for HD.…”