Production of Mycosporine-Like Amino Acids from Gracilaria vermiculophylla (Rhodophyta) Cultured Through One Year in an Integrated Multi-trophic Aquaculture (IMTA) System

Barceló-Villalobos, M.; Figueroa, Félix L.; Korbee, Nathalie; Álvarez‐Gómez, Félix; Abreu, Helena

doi:10.1007/s10126-017-9746-8

Cited by 45 publications

(29 citation statements)

References 39 publications

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“…Lalegerie et al [14] found eight different MAAs in the same species, some of them unidentified, but no total MAA concentration was shown. Barceló-Villalobos et al [32] identified four MAAs, the same as in this work, porphyra-334, shinorine, palythine, and asterina-330. However, total MAA concentration of samples collected from the field was at least two times higher than our MAA concentration.…”

Section: Discussionsupporting

confidence: 78%

“…In order to study the influence of distilled water, as a solvent of extraction in MAA analysis, four Rhodophytes were selected based on their medium-high MAA contents and their different MAA profiles [17,31,32]: Agarophyton vermiculophyllum, Crassiphycus corneus, Gracilariopsis longissima, and Pyropia leucosticta. We selected different procedures and two HPLC columns that are commonly used for MAA analysis in order to elucidate which procedure would be the best option for the isolation, identification, and quantification of MAAs for cosmetic purposes.…”

Section: Discussionmentioning

confidence: 99%

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Quantitative and Qualitative HPLC Analysis of Mycosporine-Like Amino Acids Extracted in Distilled Water for Cosmetical Uses in Four Rhodophyta

et al. 2019

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Mycosporine-like amino acids (MAAs) have gained considerable attention as highly active photoprotective candidates for human sunscreens. However, more studies are necessary to evaluate the extraction efficiencies of these metabolites in cosmetic compatible solvents, as well as, their subsequent HPLC analysis. In the present study, MAA extraction using distilled water and 20% aqueous methanol in four Rhodophyta was investigated. Different re-dissolution solvents and a C8 and C18 columns were tested for the HPLC analysis. Porphyra-334, shinorine, palythine, palythine-serine, asterina-330, and palythinol were identified by HPLC/ESI-MS. The separation of these MAAs were improved employing the C8-column, and using methanol as re-dissolution solvent. Regarding total MAAs concentrations, no differences between the two solvents were found. The highest MAA amounts were observed injecting them directly in the HPLC. According to these results, distilled water could be an excellent extraction solvent for MAAs. Nevertheless, the re-dissolution in pure methanol after dryness would be the best option for the qualitative analysis of the most common MAAs in these red algae. Our results entail important implications regarding the use of red macroalgae as promising candidates as environment-friendly sources of natural sunscreens. coefficients (ε = 28,100-50,000 L·mol −1 ·cm −1 ). They are secondary metabolites capable of absorbing UV radiation with the maximum absorbance between 310 and 365 nm [15][16][17]. MAAs have a general structure that consist of cyclohexenone or cyclohexenimine chromophores conjugated with one or two amino acids that are responsible of UV absorption [18]. The high photostability of MAAs over a wide range of temperature and pH, together with their antioxidant properties, make them promising metabolites in the biotechnology industry. These compounds are recognized as strong UV-absorbing molecules that can be used as an active ingredient in sun-care cosmetic products, therefore could be a potential supplement of chemical filters just used in sunscreens, also due to the fact that they could satisfy consumers who want the incorporation of natural ingredients. Additionally, MAAs could be effective against actinic erythema, but can also protect the humans against other biological effects such as immune suppression or photo-oxidative damage [11,[19][20][21][22][23][24][25]. So, MAAs are promising functional ingredients used for novel cosmeceuticals (cosmetic products with health benefits). In fact, MAAs have already been commercialized as Helioguard®365. This cosmetic reagent contains the MAAs, shinorine and porphyra-334, extracted from the red alga Porphyra umbilicalis and has been successfully commercialized as a natural and safe sunscreen compound. Nowadays, the MAA extraction protocols differ in type of solvents, temperature, and extraction times. The MAA extraction efficiencies and concentrations are affected by these conditions [26][27][28][29]. A variety of extraction and separation methods for MAAs have been...

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Section: Discussionsupporting

confidence: 78%

Section: Discussionmentioning

confidence: 99%

Quantitative and Qualitative HPLC Analysis of Mycosporine-Like Amino Acids Extracted in Distilled Water for Cosmetical Uses in Four Rhodophyta

et al. 2019

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“…Table 1. Structure, molar extinction coefficient, retention time, and maximum absorption wavelength of MAAs in marine macroalgae [43,95]. In addition, it is worth mentioning that the difference of the total MAAs or MAA value between cultivated marine macroalgae and field material existed from same collection location.…”

Section: Introductionmentioning

confidence: 99%

“…Table 1. Structure, molar extinction coefficient, retention time, and maximum absorption wavelength of MAAs in marine macroalgae [43,95]. In order to clearly present the distribution of specific MAA in each marine macroalgae, based on the literature information from the Web of Science, Springer, Google Scholar, and CNKI, Tables 2 and 3 are presenteed.…”

Section: Introductionmentioning

confidence: 99%

Distribution, Contents, and Types of Mycosporine-Like Amino Acids (MAAs) in Marine Macroalgae and a Database for MAAs Based on These Characteristics

et al. 2020

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Mycosporine-like amino acids (MAAs), maximally absorbed in the wavelength region of 310–360 nm, are widely distributed in algae, phytoplankton and microorganisms, as a class of possible multi-functional compounds. In this work, based on the Web of Science, Springer, Google Scholar, and China national knowledge infrastructure (CNKI), we have summarized and analyzed the studies related to MAAs in marine macroalgae over the past 30 years (1990–2019), mainly focused on MAAs distribution, contents, and types. It was confirmed that 572 species marine macroalgae contained MAAs, namely in 45 species of Chlorophytes, 41 species of Phaeophytes, and 486 species of Rhodophytes, and they respectively belonged to 28 orders. On this basis, we established an open online database to quickly retrieve MAAs in 501 species of marine macroalgae. Furthermore, research concerning MAAs in marine macroalgae were analyzed using CiteSpace. It could easily be seen that the preparation and purification of MAAs in marine macroalgae have not been intensively studied during the past 10 years, and therefore it is necessary to strengthen the research in the preparation and purification of MAA purified standards from marine macroalgae in the future. We agreed that this process is not only interesting, but important due to the potential use of MAAs as food and cosmetics, as well as within the medicine industry.

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“…In such systems, the seaweed utilizes the animals' metabolic waste as fertilizer while absorbing CO 2 and releasing O 2 to the environment. The seaweed of economic potential cultivated in IMTA is doubly interesting, since it reduces environmental impact and provides biomass for the extraction of high-value compounds (Samocha et al 2015;Yu et al 2016;Barceló-Villalobos et al 2017). In addition, IMTA has the potential to contribute to a positive, sustainable image, and improved social acceptability (Barrington et al 2010).…”

Section: Introductionmentioning

confidence: 99%

Cultivation of three color strains of Gracilaria domingensis in an integrated organic system

et al. 2017

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Species of the genus Gracilaria are widely distributed around the world, and many are wild harvested and cultivated for phycocolloid production (agar) and human food. This study investigated the growth performance of three color strains of Gracilaria domingensis in an integrated organic system. The three chromatic variants (red, green, and brown) were cultured in a shrimp farm at two depths (surface and 20 cm). The highest relative growth rates were obtained in the first week of cultivation for all three strains studied. Although the growth rates and biomass were slightly higher at a depth of 20 cm, significant differences were not observed at different depths (p \ 0.05). The growth of the three strains varied significantly over time (p \ 0.001). The maximal growth values obtained were 11.25 ± 1.50% day -1 (red), 12.76 ± 3.96% day -1 (green), and 7.86 ± 1.75% day -1 (brown). The mean growth rates for the whole period were 4.63 ± 6.97, 3.38 ± 9.09, and 2.16 ± 6.61% day -1 for the red, green, and brown strains, respectively. The pigment content (chlorophyll a, phycocyanin, and phycoerythrin) of the three strains studied was higher at a depth of 20 cm, while the maximum electron transport rate (ETR max ) and photosynthetic efficiency were higher at the surface. The high growth rates obtained in this study demonstrate the great potential of this species for integrated organic farming. Moreover, the ecological success shown by the red strain suggests that this strain is the most suitable for integrated cultivation.

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Production of Mycosporine-Like Amino Acids from Gracilaria vermiculophylla (Rhodophyta) Cultured Through One Year in an Integrated Multi-trophic Aquaculture (IMTA) System

Cited by 45 publications

References 39 publications

Quantitative and Qualitative HPLC Analysis of Mycosporine-Like Amino Acids Extracted in Distilled Water for Cosmetical Uses in Four Rhodophyta

Quantitative and Qualitative HPLC Analysis of Mycosporine-Like Amino Acids Extracted in Distilled Water for Cosmetical Uses in Four Rhodophyta

Distribution, Contents, and Types of Mycosporine-Like Amino Acids (MAAs) in Marine Macroalgae and a Database for MAAs Based on These Characteristics

Cultivation of three color strains of Gracilaria domingensis in an integrated organic system

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