Production of N-acyl homoserine lactones by Chromobacterium haemolyticum KM2 isolated from the river water in Malaysia

Priya, Kumutha; Sulaiman, Joanita; How, Kah Yan; Yin, Wai Fong; Chan, Kok‐Gan

doi:10.1007/s00203-018-1526-y

Cited by 8 publications

(5 citation statements)

References 28 publications

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“…Te growth and development of C. haemolyticum require moist water sources. Consequently, the geographical location and climate of Guangdong Province provide an ideal habitat for the reproduction of C. haemolyticum [6,15]. We also detected C. haemolyticum in the drinking water of other pig farms with unexplained diarrhea, and after changing the water source, the piglet diarrhea was controlled.…”

Section: Discussionmentioning

confidence: 64%

“…Chromobacterium haemolyticum (C. haemolyticum) was considered as a separate species, lacking violet pigmentation but demonstrating strong hemolysis on sheep blood agar plates [3]. C. haemolyticum has been isolated from a variety of water environments, such as rice roots, lakes, rivers, and sewage efuent [4][5][6]. Tere is evidence that C. haemolyticum is mainly transmitted through water sources, and some reports suggest that C. haemolyticum can cause pneumonia and sepsis in humans, and the patients in these cases have a history of wound exposure or contact with water sources, indicating that C. haemolyticum maybe an opportunistic pathogen [7,8].…”

Section: Introductionmentioning

confidence: 99%

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Outbreak of Fatal Piglet Diarrhea Caused by Chromobacterium haemolyticum in China

Zhang

Zhang²,

et al. 2023

Transboundary and Emerging Diseases

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Chromobacterium haemolyticum is a fatal Gram-negative bacterium which could infect human beings. Our investigation found severe piglet diarrhea in one farm; the morbidity rate of piglets was 30.65%. Then, we isolated a nonpigmented, β-hemolytic Gram-negative bacillus from the clinical samples of this farm, which was designated GDHYZ30 strain. The 16S rRNA gene sequence indicated it was most closely related to Chromobacterium haemolyticum. Similar clinical symptoms were successfully reproduced in experimental piglets with this isolate, and the isolate was also subjected to whole genome-sequencing. It is worth noting that this Chromobacterium haemolyticum has been isolated from several other pig farms with diarrhea of unknown causes. To our knowledge, this is the first report that discusses Chromobacterium haemolyticum as a new pathogen causing diarrhea and death in piglets and transmitting through water sources, and it provides a reference for the prevention and control of human/animal infections as well as food safety.

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Section: Discussionmentioning

confidence: 64%

Section: Introductionmentioning

confidence: 99%

Outbreak of Fatal Piglet Diarrhea Caused by Chromobacterium haemolyticum in China

Zhang

Zhang²,

et al. 2023

Transboundary and Emerging Diseases

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“…Clinical trials and drug resistance analysis show that this novel Chromobacterium haemolyticum is a potential threat to humans and animals. The suitable conditions for the growth ofChromobacterium haemolyticum are near moist water sources, and the geographical location and climate of Guangdong Province are very suitable for the reproduction of Chromobacterium haemolyticum(Lima-Bittencourt, Costa, Barbosa, Chartone-Souza, & Nascimento, 2011;Priya et al, 2018). We also detected Chromobacterium haemolyticum in the drinking water of other pig farms with unexplained diarrhea, and after changing the water source, the piglet diarrhea was controlled.…”

Section: Discussionmentioning

confidence: 69%

“…(Han, Han, & Segal, 2008). C .haemolyticum has been isolated in a variety of water environments, such as rice roots, lakes, rivers, and sewage effluent (Kanamori et al, 2020;Priya, Sulaiman, How, Yin, & Chan, 2018;Zhou et al, 2016). There is evidence that C .…”

Section: Introductionmentioning

confidence: 99%

Fatal piglets diarrhea caused by a novel Chromobacterium haemolyticum

Zhang

et al. 2022

Preprint

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Chromobacterium haemolyticum is a fatal gram-negative bacterium, which had been found that has the ability to infect human. In this study, we detected a nonpigmented, β-hemolytic gram-negative bacillus from fecal samples of piglets with severe diarrhea and death. Analysis of the 16S rRNA gene sequence indicated it was most closely related to Chromobacterium haemolyticum, however only with 79% identities, indicating that this bacterium may be a new type of Chromobacterium haemolyticum. Diarrhea was reproduced in experimental pigs with one of the isolates strains, designated GDHYZ30 strain. Intramuscular injection of the GDHYZ30 strain can cause death of the piglets, and irrigated challenge induced piglets diarrhea. To further elucidate the mechanism of pathogensis of this bacterium, whole genome sequencing was used to analyze and annotate the GDHYZ30 strain. The full-length genome of the strain was 4785117bp, the G+C content was 62.67%, and it encoded 4398 genes, in which 57 drug resistance genes, and 390 virulence factors were included. It is worth noting that this novel Chromobacterium haemolyticum has been isolated from several other pig farm water samples and pig feces samples with unexplained diarrhea. To our knowledge this is the first report that a novel Chromobacterium haemolyticum as a new pathogen causing diarrhea and death in piglets and transmiting through water sources. And the genetic characteristics of the bacteria are analyzed by whole gene sequencing, which provides a reference for the prevention of human and animal infections and food safety.

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“…AHL2 is produced by many bacteria, including Serratia spp. ( 34 , 35 ), Citrobacter amalonaticus ( 36 ), Edwardsiella tarda ( 37 ), Rhizobium leguminosarum ( 38 ), Chromobacterium haemolyticum ( 39 ), and Burkholderia cepacia ( 40 ). It is responsible for several bacterial responses such as virulence, the formation of biofilms, and swarming motility ( 40 – 42 ).…”

Section: Discussionmentioning

confidence: 99%

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

Lopez Marin,

Strejcek,

Uhlik

2023

Microbiol Spectr

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Bacteria need to be isolated in pure culture to gain access to the wide array of interesting functions they conceal. This is challenging because they do not live in isolation but instead in communities where they actively communicate and interact with each other. In this study, we aimed to increase the culturability of soil bacteria using three different signaling molecules: N-(3-oxohexanoyl)-L-homoserine lactone, N-octanoyl-L-homoserine lactone, and 3',5'-cyclic adenosine monophosphate (cAMP). The signals were added individually to soils suspended in PBS buffer to a final concentration of 5 µM. Soil suspensions were agitated for 24 hours, after which they were serially diluted and plated on tenfold-diluted Reasoner’s 2A agar that either contained or did not contain the same signaling molecule used during the extraction step. DNA was isolated from both soil suspensions and grown cultures and, after a high-throughput amplicon sequencing, differences in bacterial abundances and diversity were determined across treatments. To further explain the action of the signaling molecules on the treated soil communities, their metagenomic functions were predicted using the software PICRUSt2. N-octanoyl-L-homoserine lactone was found to increase the diversity observed on solid media, while N-(3-oxohexanoyl)-L-homoserine lactone and cAMP were not. Potentially novel isolates aided by the signaling molecules were affiliated with the genera Pseudomonas and Nocardioides . IMPORTANCE Microorganisms are a repository of interesting metabolites and functions. Therefore, accessing them is an important exercise for advancing not only basic questions about their physiology but also to advance technological applications. In this sense, increasing the culturability of environmental microorganisms remains an important endeavor for modern microbiology. Because microorganisms do not live in isolation in their environments, molecules can be added to the cultivation strategies to “inform them” that they are present in growth-permissive environmental conditions. Signaling molecules such as acyl-homoserine lactones and 3',5'-cyclic adenosine monophosphate belong to the plethora of molecules used by bacteria to communicate with each other in a phenomenon called quorum sensing. Therefore, including quorum sensing molecules can be an incentive for microorganisms, specifically soil bacteria, to increase their numbers on solid media.

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Production of N-acyl homoserine lactones by Chromobacterium haemolyticum KM2 isolated from the river water in Malaysia

Cited by 8 publications

References 28 publications

Outbreak of Fatal Piglet Diarrhea Caused by Chromobacterium haemolyticum in China

Outbreak of Fatal Piglet Diarrhea Caused by Chromobacterium haemolyticum in China

Fatal piglets diarrhea caused by a novel Chromobacterium haemolyticum

Joining the bacterial conversation: increasing the cultivation efficiency of soil bacteria with acyl-homoserine lactones and cAMP

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