Production of natural products by plant cell biotechnology: results, problems and perspectives

Alfermann, A. W.; Petersen, Maike; Fuss, Elizabeth

doi:10.1007/978-3-7091-6040-4_9

Cited by 14 publications

(9 citation statements)

References 52 publications

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“…The biomass of the microrhizomes was related to sugar concentration in medium, and the larger microrhizomes can be directly field planted without an acclimatization treatment (Shirgukar et al, 2001). of attempts, there are only four commercially successful applications (Alferman et al, 2003). Two plausible explanations for so many disappointing results are 1) cell culture fermentors are too expensive, and 2) nondifferentiated cells in liquid medium do not accumulate adequate quantities of the desired compounds (Preil, 2005 Bioreactor systems that aerate large quantities of liquid tissue culture medium were developed for both micropropation and direct production of phytochemicals (Preil 2005).…”

mentioning

confidence: 99%

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Adelberg¹,

Cousins²

2006

HortSci

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Geophytes store carbohydrates in modified underground shoot systems protected by a broad array of biologically active chemistry. In vitro formation of storage organs requires months in the lab instead of years in the field, when water and nutrients are correctly supplied. Liquid and agar systems in large and small vessels were compared for sugar and water use with turmeric (Curcuma longa) as a model plant. Small jars on a shaker were compared with large, flat-bottomed vessels containing thin films of liquid media, intermittently tilted at slight inclines that allow the advantages of liquid phase transfer with gentle agitation. Liquid culture in small vessels on a shaker yielded the most plants and liquid culture on a thin-film rocker in a large vessel yielded the largest plants. Increased and improved biomass (fresh and dry) in liquid culture compared to agar was based on greater sugar use. When large vessels of liquid media were grown for 5 and 6 months on a rocker, 400 mL of media yielded 150 to 200 g (fresh weight) of plants. Similarly, 13 to 16 g (dry weight) of plant tissue was derived from 24 g of sugar. Plants were about one-third rhizome by fresh mass. Rhizomes had greater dry and fresh weight than leaves or roots, indicating solute actively accumulated in the rhizome. The rhizomes had normal morphology, characteristic pigments and fragrance, and rhizome extracts had strong antioxidant potential. The gentle rocking action of plantlets in sugar-containing liquid medium was demonstrated to produce functional storage organs.

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confidence: 99%

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Adelberg¹,

Cousins²

2006

HortSci

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“…One major problem of plant regeneration from somatic embryos is their limited amount of available storage compounds, lacking the extra-embyonal reservoir zygotic embryos have at their disposal (Alferman et al, 2003). Most of papers focused in demonstrating those compounds limitations, however, their distribution is not taking into account.…”

Section: Discussionmentioning

confidence: 99%

Polyphenols Distribution and Reserve Substances Analysis in Cocoa Somatic Embryogenesis

et al. 2016

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In order to understand the causes of lack of regeneration in cacao somatic embryos, two cacao varieties with different responses to regeneration potential were described based on their capacity to store different compounds. It is well known that seed reserves play a central role in the regenerative capability of somatic embryos; thus, we followed histochemical changes and reserve fluctuations of proteins, polysaccharides and polyphenols during somatic embryogenesis (SE) in the two cacao varieties. The study showed that, in somatic embryos of the regenerating variety, polyphenols were localized mainly in the periphery of the embryo (epidermal cells) and proteins were the main storage substance in the embryo expression medium, while the non-regenerating variety had a high presence of polysaccharides with random distribution of polyphenols at the end of the embryo induction step.Keywords: antioxidants, histology, reserve accumulation, recalcitrance, somatic embryogenesis. RESUMENDos variedades de cacao con diferentes respuestas a la regeneración fueron descritas en función de su capacidad para almacenar diferentes compuestos, con el fin de aproximarse al entendimiento de las causas de la falta de regeneración en embriones somáticos de cacao. Es bien sabido que las reservas de semillas desempeñan un papel central en la capacidad de regeneración de embriones somáticos; por tanto, se realizó un seguimiento de cambios histoquímicos y fluctuaciones de reserva de proteínas, polisacáridos y polifenoles durante la embriogénesis somática (SE) en dos variedades de cacao. El estudio mostró que, en los embriones somáticos de la variedad regenerante, los polifenoles se localizaron principalmente en la periferia del embrión (células de la epidermis) y las proteínas fueron el componente principal de almacenamiento en el medio de expresión de embriones, mientras que la variedad no regenerante tenía una alta presencia de polisacáridos y una distribución aleatoria de los polifenoles en el final de la etapa de inducción de embriones. Palabras clave: acumulación de reservas, antioxidantes, embriogénesis somática, histología, recalcitrancia.

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“…Plant cell cultures offer an alternative source to the whole plant for the production of high-value secondary metabolites [5]. Researchers have endeavored to utilize plant cell biosynthetic capabilities for obtaining useful products and for studying the metabolism [6].…”

Section: Introductionmentioning

confidence: 99%

Rapid in Vitro Callogenesis and Phytochemical Screening of Leaf, Stem and Leaf Callus of Mussaenda Frondosa Linn. - A Medicinal Plant.

Chandrashekar

Bhagya

2017

Asian J Pharm Clin Res

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Objective: To standardize the protocol for rapid callogenesis in Mussaenda frondosa L. using leaf explants. Qualitative and quantitative phytochemical analysis of leaf, stem, and callus cultures. Methods:The leaf explants were inoculated onto Murashige and Skoog medium supplemented with varying concentrations of growth regulators such as 2, 4-D, NAA, benzylaminopurine, Kn for the induction of callus. Qualitative and quantitative analysis of total phenol, flavonoid and alkaloid contents of leaf, stem, and callus were tested by standard methods. The antioxidant activities were investigated using 1, 1-diphenyl-2-picryl hydrazyl radical scavenging method and reducing power assay. The anti-inflammatory activity was evaluated by membrane stabilizing activity.Results: Pale green, healthy, friable, and fast growing callus were obtained on the medium enriched with NAA (2 mg/l)+Kn (4 mg/l). Quantitative determination showed the highest concentration of total phenolics in the methanolic extract of in vitro grown callus (10±1.1 mg of GA/g of extract), flavonoids in methanolic stem extract (137±1.6 mg of quercitin/g of extract) and alkaloids in methanolic extract of leaf (118.3±1.5 mg/10 g of extract). The methanolic leaf extract exhibited the highest free radical scavenging activity with an inhibitory concentration 50% (IC 50 ) value of 40.6±10.06 μg/ml. The highest membrane stabilizing activity was shown by chloroform extract of the leaf (66.02%). Conclusion:This preliminary phytochemical and pharmacological analysis may form the basis for drug development in future using callus cultures of M. frondosa.

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Production of natural products by plant cell biotechnology: results, problems and perspectives

Abstract: This short review presents some examples, which demonstrate the importance and the potential of plant cell and tissue cultures

Cited by 14 publications

References 52 publications

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Thin Films of Liquid Media for Heterotrophic Growth and Storage Organ Development: Turmeric (Curcuma longa) as a Model Plant

Polyphenols Distribution and Reserve Substances Analysis in Cocoa Somatic Embryogenesis

Rapid in Vitro Callogenesis and Phytochemical Screening of Leaf, Stem and Leaf Callus of Mussaenda Frondosa Linn. - A Medicinal Plant.

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