Production of New Allotetraploid and Autotetraploid Citrus Breeding Parents: Focus on Zipperskin Mandarins

Grosser, Jude W.; An, Hyun Joo; Ćalović, Milica; Lee, Dong Hoon; Chen, Chunxian; Vasconcellos, Marco Antônio da Silva; Gmitter, Frederick G.

doi:10.21273/hortsci.45.8.1160

Cited by 24 publications

(16 citation statements)

References 25 publications

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“…Due to constraints of the citrus reproductive system, such as polyembryony in most mandarins, specific characteristics or capacity to produce triploid hybrids (Aleza et al, 2010b), several ABS-susceptible cultivars are being used as parents in many mandarin breeding programs, both at diploid and triploid level (McCollum, 2008; Aleza et al, 2010a,b; Cuenca et al, 2010; Grosser et al, 2010; Aleza et al, 2012). This is the case of the monoembryonic cultivar “Fortune,” widely used as female parent in the triploid breeding programs in Spain (Navarro et al, 2012), France and Italy (Recupero et al, 2005; Froelicher et al, 2012); the susceptible cultivars “Murcott” and “Ponkan” are widely used as male parents in many diploid breeding programs carried out in Japan and Brazil (JinPing et al, 2009; Schinor et al, 2012).…”

Section: Introductionmentioning

confidence: 99%

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

et al. 2016

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Alternaria brown spot (ABS) is a serious disease affecting susceptible citrus genotypes, which is a strong concern regarding citrus breeding programs. Resistance is conferred by a recessive locus (ABSr) previously located by our group within a 3.3 Mb genome region near the centromere in chromosome III. This work addresses fine-linkage mapping of this region for identifying candidate resistance genes and develops new molecular markers for ABS-resistance effective marker-assisted selection (MAS). Markers closely linked to ABSr locus were used for fine mapping using a 268-segregating diploid progeny derived from a heterozygous susceptible × resistant cross. Fine mapping limited the genomic region containing the ABSr resistance gene to 366 kb, flanked by markers at 0.4 and 0.7 cM. This region contains nine genes related to pathogen resistance. Among them, eight are resistance (R) gene homologs, with two of them harboring a serine/threonine protein kinase domain. These two genes along with a gene encoding a S-adenosyl-L-methionine-dependent-methyltransferase protein, should be considered as strong candidates for ABS-resistance. Moreover, the closest SNP was genotyped in 40 citrus varieties, revealing very high association with the resistant/susceptible phenotype. This new marker is currently used in our citrus breeding program for ABS-resistant parent and cultivar selection, at diploid, triploid and tetraploid level.

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Section: Introductionmentioning

confidence: 99%

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

et al. 2016

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“…And polyploids of fruit crops possibly produced seedless fruits, which are very desirable especially in edible fruits with hard seeds such as orange, grape, grapefruit, pineapple, and banana. Many neopolyploids have been obtained from recent explorations using laboratory-generated or synthetic allopolyploids, which were used for genome evolution research (Parisod et al, 2010;Yang et al, 2011) or breeding purposes (Grosser et al, 2010;Chae et al, 2013), development of new varieties and new germplasm (Sedov et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Field performance of vegetative form traits of neopolyploids produced by in vitro colchicine treatment in Pyrus communis

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et al. 2015

Scientia Horticulturae

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“…For mandarin improvement, additional breeding objectives beyond seedlessness include easy-peeling, good external/ internal color, good flavor, a range of maturity dates, and good shipping ability/shelf-life. Our program is continuing efforts to generate improved breeding parents, and we recently reported 9 new somatic hybrids and 5 autotetraploids produced by protoplast fusion, with focus on the zipper-skin trait (Grosser et al 2010).…”

Section: Ploidy Manipulation In Scion Improvementmentioning

confidence: 99%

Protoplast fusion for production of tetraploids and triploids: applications for scion and rootstock breeding in citrus

Grosser

Gmitter

2010

Plant Cell Tiss Organ Cult

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Protoplast fusion technology has been utilized in many crops to generate allotetraploid somatic hybrids, and sometimes autotetraploids as a byproduct of the process. A brief history of this technology development is provided, along with a simple protocol developed for citrus, which can be easily adapted to other plants. Protoplast fusion has become a significant tool in ploidy manipulation that can be applied in various cultivar improvement schemes. In rare cases, a new somatic hybrid may have direct utility as an improved cultivar; however, the most important application of somatic hybridization is the building of novel germplasm as a source of elite breeding parents for various types of conventional crosses for both scion and rootstock improvement. Somatic hybridization is generating superior allotetraploid breeding parents for use in interploid crosses to generate seedless triploids. Seedlessness is a primary breeding objective for new fresh fruit citrus varieties, and several thousand triploid hybrids have been produced using somatic hybrids as the tetraploid parent. Protoplast fusion is also being utilized to produce somatic hybrids that combine complementary diploid rootstocks, which have shown good potential for tree size control. Tree size control has gained importance as a means of reducing harvesting costs, maximizing the efficiency of modern cold protection methodology, and facilitating the adaptation of new fruit production systems. Successful somatic hybridization in citrus rootstock improvement has enabled rootstock breeding at the tetraploid level via sexual hybridization, which can yield maximum genetic diversity in zygotic progeny upon which to impose selection for the many traits required in improved rootstock cultivars, including disease and insect resistance, broad adaptation, tree size control, and the ability to consistently produce high yields of quality fruit. Recent progress and successful examples of these applications are discussed. Finally, a discussion of the genetic potential of somatic hybrids as breeding parents, including meiotic behavior and inheritance is provided.

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Production of New Allotetraploid and Autotetraploid Citrus Breeding Parents: Focus on Zipperskin Mandarins

Cited by 24 publications

References 25 publications

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Fine Mapping for Identification of Citrus Alternaria Brown Spot Candidate Resistance Genes and Development of New SNP Markers for Marker-Assisted Selection

Field performance of vegetative form traits of neopolyploids produced by in vitro colchicine treatment in Pyrus communis

Protoplast fusion for production of tetraploids and triploids: applications for scion and rootstock breeding in citrus

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