Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells

Li, Zhengyi; Xiao, Min; Lu, Lili; Li, Yumei

doi:10.1016/j.procbio.2008.04.016

Cited by 78 publications

(50 citation statements)

References 18 publications

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“…In a previous research, Kluyveromyces marxianus improved the purity of a GOS mixture produced by β-galactosidase from B. circulans from 38 to 97 % by selective fermentation of mono-and disaccharides (including lactose) (6). With a combined use of S. cerevisiae and K. lactis the purity of a GOS mixture produced by β-galac tosidase from Penicillium expansum increased from 29 to 98 % (8). In this work, HP-GOS were produced by fermentation with S. cerevisiae ( Table 1 and Fig.…”

Section: Gos Content Before and After Enzymatic Hydrolysis And Fermenmentioning

confidence: 93%

“…Cardelle-Cobas et al (7) fi rst reported the production of non-monosaccharides and HP-GOS by repeated batch fermentation with immobilized yeast cells. A GOS syrup of an increased purity was produced by immobilized β-galactosidase from Penicillium expansum F3 and subjected to fermentation by Saccharomyces cerevisiae L1 and Kluyveromyces lactis L3 (8). This was a feasible industrial process to produce high-purity GOS.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo

Hong

Kim

Kwon

et al. 2016

Food Technol. Biotechnol.

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SummaryGalactooligosaccharides (GOS) are an important class of dietary prebiotics that exert benefi cial eff ects on intestinal microbiota and gut barrier function. In this study, high-purity GOS (HP-GOS) were investigated in vitro and in vivo and confi rmed as prebiotic ingredients in rat diet. HP-GOS were successfully produced using a two-step process, enzymatic hydrolysis and fermentation by yeast. They were found to serve as a good substrate and carbon source for supporting the growth of probiotic bacteria more eff ectively than other commercial GOS. Following administration of 1 % (by mass) of HP-GOS to rats, the growth of Bifi dobacterium bifi dum and B. longum in the gut increased most rapidly up to 12 h, and thereaft er the increase was slow. Therefore, 1 % HP-GOS was found to be acceptable for the growth of probiotic bacteria. Groups of animals that were orally administered HP-GOS and bifi dobacteria during the study, and the group administered HP-GOS during the 2nd (days 13-15) and 4th (days 28-30) period of the study had signifi cantly (p<0.05) higher numbers of bifi dobacteria in faeces than groups receiving a single dose of bifi dobacteria. HP-GOS aff ected the expression of genes encoding glucagon-like peptide-1 (GLP-1) and peptide YY (PYY). There was a signifi cant upregulation of GLP-1 and PYY mRNA with HP-GOS and bifi dobacteria intake. We propose that the prebiotic properties of HP-GOS are potentially valuable for the production of functional foods for human consumption.

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Section: Gos Content Before and After Enzymatic Hydrolysis And Fermenmentioning

confidence: 93%

Section: Introductionmentioning

confidence: 99%

Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo

Hong

Kim

Kwon

et al. 2016

Food Technol. Biotechnol.

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“…In addition, the GOS yield remained at high levels even after seven rounds of batch synthesis (40.80%), with 92% of the initial yield (43.64%). This is considerably higher than the 24.6% GOS yield from wild-type immobilized enzyme that had 85% of the initial yield (28.7%) (9). In previous reports, the yield of GOS after seven batches was less than 90% of the initial yield (1,20).…”

Section: Discussionmentioning

confidence: 60%

“…Samples from the culture broth (1 ml) were periodically withdrawn to analyze the GOS yield. The yield of GOS was monitored by highperformance liquid chromatography as described in our previous study (9). After one batch synthesis of GOS, the culture broth remaining in the flask was centrifuged (3 min at 3,000 ϫ g and 4°C) to separate the GOS solution from the BGase-anchored yeast cells.…”

Section: Methodsmentioning

confidence: 99%

Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

Wang

et al. 2009

Appl Environ Microbiol

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“…As for non-immobilized enzymes, the predominant reaction products are trisaccharides, namely 4 0 -or 6 0 -galactosyllactose, and longer oligosaccharides consisting of 4 or more monosaccharide units (Albayrak & Yang, 2002;Li, Xiao, Lu, & Li., 2008;Shin, Park, & Yang, 1998). Substantial amounts of transgalactosylated disaccharides are also produced in these reactions Ito et al, 1990).…”

Section: Introductionmentioning

confidence: 99%

Characterization of galactooligosaccharides produced by β-galactosidase immobilized onto magnetized Dacron

Neri

Balcão

Cardoso

et al. 2011

International Dairy Journal

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a b s t r a c tBioconversions using enzymes immobilized in magnetic supports present significant advantages due to the easy separation of the enzyme from the reaction mixture and the simplicity and low cost of the support preparation. The characterization of the oligosaccharide mixture obtained by the action of b-galactosidase covalently attached, via glutaraldehyde, to a hydrazideeDacronemagnetite composite is presented. The fractionation of the oligosaccharide mixture by high performance liquid chromatography, followed by the analysis of the purified compounds by mass spectrometry and nuclear magnetic resonance spectroscopy permitted the identification of glucose, galactose, lactose and a hexose disaccharide containing a 1/6 linkage. Also, the following GOS were identified: b-D-Galp-(1/6)-b-D-Galp-(1/4)-Glcp, b-D-Galp-(1/4)-[b-D-Galp-(1/6)]-Glcp and b-D-Galp-(1/6)-b-D-Galp-(1/6)-b-D-Galp-(1/4)-Glcp. When GOS yield (26.2%) and kinetics of biotransformation of lactose by the Dacron immobilized b-galactosidase were compared with values obtained for the enzyme immobilized in other magnetic supports, similar behaviour was observed.

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Production of non-monosaccharide and high-purity galactooligosaccharides by immobilized enzyme catalysis and fermentation with immobilized yeast cells

Cited by 78 publications

References 18 publications

Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo

Evaluation of Prebiotic Effects of High-Purity Galactooligosaccharides in vitro and in vivo

Cell Surface Engineering of a β-Galactosidase for Galactooligosaccharide Synthesis

Characterization of galactooligosaccharides produced by β-galactosidase immobilized onto magnetized Dacron

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