Production of novel antibiotic, dopsisamine, by a new subspecies of Nocardiopsis mutabilis with multiple antibiotic resistance.

Takahashi, Atsushi; Hottan, Kunimoto; Saito, Oriko; Morioka, Moto; Okami, Yoshirō; Umezawa, Hamao

doi:10.7164/antibiotics.39.175

Cited by 31 publications

(15 citation statements)

References 13 publications

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“…At the time of writing, the genus Saccharothrix encompasses 21 species and 2 subspecies with validly published names, in which eight species were isolated from deserts (Liu et al, 2020c). The genus Saccharothrix was found to be a potential producer of novel specialized metabolites, and these chemical entities exhibited a broad range of biological functions, such as antibacterial (Takahashi et al, 1986;Isshiki et al, 1989;Takeuchi et al, 1992), antifungal (Igarashi et al, 1997), antitumor (Vértesy et al, 2001), antivirus (Tomita et al, 1991), pro-inflammatory (Gibson et al, 2005), and other activities (Kimura et al, 1995;Yoshimura et al, 1995;Koguchi et al, 2000) of medical and industrial importance. For instance, at least 14 dithiolopyrrolone compounds with strong activities against a panel of bacteria, yeasts, and filamentous fungi were isolated naturally or using biosynthetic method from Saccharothrix algeriensis NRRL B-24137 (Lamari et al, 2002;Bouras et al, 2008;Merrouche et al, 2010Merrouche et al, , 2011.…”

Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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Taklamakan desert is known as the largest dunefield in China and as the second largest shifting sand desert in the world. Although with long history and glorious culture, the Taklamakan desert remains largely unexplored and numerous microorganisms have not been harvested in culture or taxonomically identified yet. The main objective of this study is to explore the diversity, novelty, and pharmacological potential of the cultivable actinomycetes from soil samples at various sites along the Alar-Hotan desert highway in the Taklamakan desert. A total of 590 actinobacterial strains were recovered by the culture-dependent approach. Phylogenetic analysis based on 16S ribosomal RNA (rRNA) gene sequences unveiled a significant level of actinobacterial diversity with 55 genera distributed in 27 families of 12 orders. Thirty-six strains showed relatively low 16S rRNA similarities (<98.65%) with validly described species, among which four strains had already been characterized as novel taxa by our previous research. One hundred and forty-six actinobacterial isolates were selected as representatives to evaluate the antibacterial activities and mechanism of action by the paper-disk diffusion method and a double fluorescent protein reporter “pDualrep2” system, respectively. A total of 61 isolates exhibited antagonistic activity against the tested “ESKAPE” pathogens, among which seven strains could produce bioactive metabolites either to be able to block translation machinery or to induce SOS-response in the pDualrep2 system. Notably, Saccharothrix sp. 16Sb2-4, harboring a promising antibacterial potential with the mechanism of interfering with protein translation, was analyzed in detail to gain deeper insights into its bioactive metabolites. Through ultra-performance liquid chromatography (UPLC)-quadrupole time-of-flight (QToF)-MS/MS based molecular networking analysis and databases identification, four families of compounds (1–16) were putatively identified. Subsequent bioassay-guided separation resulted in purification of four 16-membered macrolide antibiotics, aldgamycin H (8), aldgamycin K (9), aldgamycin G (10), and swalpamycin B (11), and their structures were elucidated by HR-electrospray ionization source (ESI)-MS and NMR spectroscopy. All compounds 8–11 displayed antibacterial activities by inhibiting protein synthesis in the pDualrep2 system. In conclusion, this work demonstrates that Taklamakan desert is a potentially unique reservoir of versatile actinobacteria, which can be a promising source for discovery of novel species and diverse bioactive compounds.

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Section: Discussionmentioning

confidence: 99%

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Liu

Wang

et al. 2021

Front. Microbiol.

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“…Saccharothrix species have been reported to produce antibiotics belonging to aminoglycoside and benzoquinone (Takahashi et al 1986), glycopeptide (Takeuchi et al 1992), carboxylic nucleoside (Bush et al 1993), dithiolopyrrolone (Lamari et al 2002), heptadecaglycoside (Singh et al 2000), anthracyclin (Zitouni et al 2004b), macrolide (Murakami et al 2009) and angucycline (Kalinovskaya et al 2010) families. The fact that Saccharothrix produces chloramphenicol is itself quite original.…”

Section: Spectroscopic Analysis Of Antibioticsmentioning

confidence: 99%

Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil

Aouiche

Sabaou

Meklat

et al. 2011

World J Microbiol Biotechnol

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An actinomycete strain designated PAL54, producing an antibacterial substance, was isolated from a Saharan soil in Ghardaïa, Algeria. Morphological and chemical studies indicated that this strain belonged to the genus Saccharothrix. Analysis of the 16S rDNA sequence showed a similarity level ranging between 96.9 and 99.2% within Saccharothrix species, with S. longispora DSM 43749(T), the most closely related. DNA-DNA hybridization confirmed that strain PAL54 belonged to Saccharothrix longispora. It showed very strong activity against pathogenic Gram-positive and Gram-negative bacteria responsible for nosocomial infections and resistant to multiple antibiotics. Strain PAL54 secreted the antibiotic optimally during mid-stationary and decline phases of growth. One antibacterial compound was isolated from the culture broth and purified by HPLC. The active compound was elucidated by uv-visible and NMR spectroscopy and by mass spectrometry. The results showed that this compound was a D: (-)-threo chloramphenicol. This is the first report of chloramphenicol production by a Saccharothrix species.

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“…Produces the antibiotic polynitoxin. The type strain of S. mutabilis is strain NRRL B-16077 (= ATCC A strain previously described (17) as " N . rnutabilis subsp.…”

Section: Discussionmentioning

confidence: 99%

Amendment of the Genus Saccharothrix Labeda et al. 1984 and Descriptions of Saccharothrix espanaensis sp. nov., Saccharothrix cryophilis sp. nov., and Saccharothrix mutabilis comb. nov.

Labeda

Lechevalier

1989

International Journal of Systematic Bacteriology

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Production of novel antibiotic, dopsisamine, by a new subspecies of Nocardiopsis mutabilis with multiple antibiotic resistance.

Cited by 31 publications

References 13 publications

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Bioprospecting of Soil-Derived Actinobacteria Along the Alar-Hotan Desert Highway in the Taklamakan Desert

Saccharothrix sp. PAL54, a new chloramphenicol-producing strain isolated from a Saharan soil

Amendment of the Genus Saccharothrix Labeda et al. 1984 and Descriptions of Saccharothrix espanaensis sp. nov., Saccharothrix cryophilis sp. nov., and Saccharothrix mutabilis comb. nov.

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