Production of oligocellouronates by biodegradation of oxidized cellulose

Delattre, Cédric; Michaud, Philippe; Elboutachfaiti, Redouan; Courtois, B.; Courtois, J.

doi:10.1007/s10570-005-9031-2

Cited by 29 publications

(20 citation statements)

References 13 publications

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“…Previously, an endo-GL acting on bacterial O-acetylated ␤-(134)-glucuronan was purified from Trichoderma sp. as a 27-kDa protein (7,9), and this protein may be a homologue of the GL from T. reesei described here. The activity of TrGL in …”

Section: Discussionmentioning

confidence: 70%

Cloning of the Trichoderma reesei cDNA Encoding a Glucuronan Lyase Belonging to a Novel Polysaccharide Lyase Family

Konno

Igarashi

Habu

et al. 2009

Appl Environ Microbiol

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The filamentous fungus Trichoderma reesei produces glucuronan lyase (TrGL) when it is grown on ␤-(134)-polyglucuronate (cellouronate) as a sole carbon source. The cDNA encoding TrGL was cloned, and the recombinant enzyme was heterologously expressed in Pichia pastoris. The cDNA of TrGL includes a 777-bp open reading frame encoding a 20-amino-acid signal peptide and the 238-amino-acid mature protein. The amino acid sequence showed no similarity to the amino acid sequences of previously described functional proteins, indicating that the enzyme should be classified in a novel polysaccharide lyase (PL) family. Recombinant TrGL catalyzed depolymerization of cellouronate endolytically by ␤-elimination and was highly specific for cellouronate. The enzyme was most active at pH 6.5 and 50°C, and its activity and thermostability increased in the presence of Ca 2؉ , suggesting that its calcium dependence is similar to that of other PLs, such as pectate lyases.

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Section: Discussionmentioning

confidence: 70%

Cloning of the Trichoderma reesei cDNA Encoding a Glucuronan Lyase Belonging to a Novel Polysaccharide Lyase Family

Konno

Igarashi

Habu

et al. 2009

Appl Environ Microbiol

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“…This new fungal glucuronan lyase degraded all glucuronans (acetylated or not), ulvan extracted from the green seaweed Ulva lactuca and also cellouronate. [71,55]. This endolytic enzyme (GL2) with a molecular weight of 27 kDa had a higher specific activity than the one from the S. meliloti M5N1CS strain.…”

Section: Glucuronan/cellouronate Acting En-zymesmentioning

confidence: 89%

“…This characteristic opens the way to applications in gasbarrier biomaterial and constitutes environmentally friendly biodegradable packaging films. Studies have led to the biodegradation of these synthetic polyuronides using new specific polysaccharide cleavage enzymes as polysaccharide lyases or hydrolases in order to consider large productions of potential bioactive anionic oligosaccharides [55,56]. The use of these enzymatic activities could also generate new oligosaccharides with biological activities as described on lots of organisms such as bacteria, fungi, plant, algae and mammalian [57,58].…”

Section: Tempo/nabr/naocl System Mediated Oxida-tion Of Polysaccharidesmentioning

confidence: 99%

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β -(1,4)-Polyglucuronic Acids – An Overview

Tavernier¹,

Delattre²,

Petit³

et al. 2008

TOBIOTJ

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Abstract:Polyuronides are an acidic class of polysaccharides with interesting rheological and biological properties. However, except pectin and alginate, the structural variability of this class of polysaccharides is poor and low described in literature. In this context, a new generation of polyuronides has been isolated from two sources in the middle of the 90's. Firstly, a bacterial -(1,4) polyglucuronic acid called glucuronan was identified as the sole exopolysaccharide produced by a bacteria belonging to the Rhizobiaceae family. Secondly, the development of the TEMPO chemistry led to the production at large scale of oxidized cellulose called cellouronate. Both new polyuronides were largely patented and found applications in several industrial areas. Moreover, the biodegradation study of these polysaccharides has led to the identification of a new family of polysaccharide lyases very specific for these substrates. This review focuses on the actual knowledge of this class of acidic polysaccharides and on the enzymes acting about them.

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“…For example, the sulfatation of hydroxyl groups is interesting since significant effects on the physiological functions of polysaccharides have been described such as anticoagulant, antitumor, and anti-HIV infection activities (Martinichen-Herreroa, Carboneroa, Gorina, & Iacominia, 2005;Wang, Zhang, Li, Hou, & Zeng, 2004). Moreover, carboxylation of polysaccharides provides a fast and useful way to improve the water solubility and increase biological activity of native polysaccharides (Delattre, Michaud, Elboutachfaiti, Courtois, & Courtois, 2006;Fan, Saito, & Isogai, 2009;Fraschini & Vignon, 2000;Isogai & Kato, 1998;Muzzarellia, Muzzarellia, Cosanib, & Terbojevichb, 1999). These last years, a specific catalytic oxidation of C6 primary hydroxyl groups of polysaccharides by the stable nitroxyl radical 2,2,6,6-tetramethylpiperidine-1-oxyl radical (TEMPO) and NaOCl was abundantly described for its high regioselectivity and reaction rate (Bragd, van Bekkum, & Besemer, 2004;De Nooy, Besemer, & Van Bekkum, 1995).…”

Section: Introductionmentioning

confidence: 99%