Production of phycocyanin by Leptolyngbya sp. in desert environments

Schipper, Kira; Fortunati, Filippo; Oostlander, P.C.; Muraikhi, Mariam Al; Jabri, Hareb Mohammed S.J. Al; Wijffels, René H.; Barbosa, María J.

doi:10.1016/j.algal.2020.101875

Cited by 36 publications

(29 citation statements)

References 41 publications

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“…to produce PC in continuous culture at 80 μmol photons m −2 s −1 . Under these conditions, 86 mg of PC g −1 biomass was produced with a productivity of 78.8 mg L −1 d −1 [ 24 ].…”

Section: Discussionmentioning

confidence: 99%

Continuous Microalgal Cultivation for Antioxidants Production

et al. 2020

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Microalgae, including cyanobacteria, represent a valuable source of natural compounds that have remarkable bioactive properties. Each microalga species produces a mixture of antioxidants with different amounts of each compound. Three aspects are important in the production of bioactive compounds: the microalga species, the medium composition including light supplied and the photobioreactor design, and operation characteristics. In this study, the antioxidant content and productivity performance of four microalgae were assessed in batch and continuous cultures. Biomass productivity by the four microalgae was substantially enhanced under continuous cultivation by 5.9 to 6.3 times in comparison with batch cultures. The energetic yield, under the experimental conditions studied, ranged from 0.03 to 0.041 g biomass kJ−1. Phenols, terpenoids, and alkaloids were produced by Spirulinaplatensis, Isochrysisgalbana, and Tetraselmissuecica, whereas tocopherols and carotenoids were produced by the four microalgae, except for phycocyanin and allophycocyanin, which were only produced by S. platensis and Porphyridiumcruentum. The findings demonstrate that the continuous cultivation of microalgae in photobioreactors is a convenient method of efficiently producing antioxidants.

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Section: Discussionmentioning

confidence: 99%

Continuous Microalgal Cultivation for Antioxidants Production

et al. 2020

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“…Chlorophyll content (

X_{Chl}

, mg Chl /g X ) was determined by dividing the chlorophyll concentration with the biomass concentration (

C_{x}

, g X /L). Phycocyanin extraction was performed using phosphate buffer and bead beating, followed by 24 h incubation at 4°C, and spectrophotometric analysis, according to the method described by Schipper et al, 2020.…”

Section: Methodsmentioning

confidence: 99%

“…Qatar, a peninsula located in the Arabian Gulf, is one such location, and a number of high‐potential strains have been isolated from the region (Das et al, 2019, Saadaoui et al, 2016, Schipper et al, 2019). One strain, in particular, has already been investigated for its potential to produce phycocyanin‐rich biomass under simulated desert climate conditions, and only limited photoinhibition was observed at high light intensities of 1800 μmol photons/m 2 /s (Schipper et al, 2020). This study, therefore, focuses on the scale‐up and outdoor cultivation of Leptolyngbya sp.…”

Section: Introductionmentioning

confidence: 99%

Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation

Schipper

Das

Muraikhi

et al. 2021

Biotech & Bioengineering

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The effect of light intensity and inoculum volume on the occurrence of photooxidation for Leptolyngbya sp. QUCCCM 56 was investigated, to facilitate the transition from small-scale laboratory experiments to large-scale outdoor cultivation. Indoor, the strain was capable of growing at light intensities of up to 5600 µmol photons/m 2 /s, at inoculation densities as low as 0.1 g/L (10% inoculation volume vol/vol). Levels of chlorophyll and phycocyanin showed a significant decrease within the first 24 h, indicating some level of photooxidation, however, both were able to recover within 72 h. When cultivated under outdoor conditions in Qatar during summer, with average peak light intensities 1981 ± 41 μmol photons/m 2 /s, the strain had difficulties growing. The culture recovered after an initial adaptation period, and clear morphological differences were observed, such as an increase in trichome length, as well as coiling of multiple trichomes in tightly packed strands. It was hypothesized that the morphological changes were induced by UV-radiation as an adaptation mechanism for increased self-shading. Furthermore, the presence of contaminating ciliates could have also affected the outdoor culture. Both UV and contaminants are generally not simulated under laboratory environments, causing a mismatch between indoor optimizations and outdoor realizations.

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“…Phycocyanin is a pigment–protein complex found in cyanobacteria and eukaryotic algae that functions as a light-harvesting pigment. It is widely used in biotechnological, food and pharmaceutical industries [ 87 ]. Schipper et al analyzed the phycocyanin content in Leptolyngbya sp.…”

Section: Chemical Diversity Of the Secondary Metabolites Isolated mentioning

confidence: 99%

“…Schipper et al analyzed the phycocyanin content in Leptolyngbya sp. QUCCCM 56 from a desert environment to reveal that it possesses higher and purer phycocyanin compared to the current commercial source, Arthrospira platensis [ 87 ]. Other than absorbing light, phycocyanin also shows potential anti-aging and proteostasis-suppressive activities.…”

Section: Chemical Diversity Of the Secondary Metabolites Isolated mentioning

confidence: 99%

The Chemistry, Biochemistry and Pharmacology of Marine Natural Products from Leptolyngbya, a Chemically Endowed Genus of Cyanobacteria

Naman

Alexander

et al. 2020

Marine Drugs

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Leptolyngbya, a well-known genus of cyanobacteria, is found in various ecological habitats including marine, fresh water, swamps, and rice fields. Species of this genus are associated with many ecological phenomena such as nitrogen fixation, primary productivity through photosynthesis and algal blooms. As a result, there have been a number of investigations of the ecology, natural product chemistry, and biological characteristics of members of this genus. In general, the secondary metabolites of cyanobacteria are considered to be rich sources for drug discovery and development. In this review, the secondary metabolites reported in marine Leptolyngbya with their associated biological activities or interesting biosynthetic pathways are reviewed, and new insights and perspectives on their metabolic capacities are gained.

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Production of phycocyanin by Leptolyngbya sp. in desert environments

Cited by 36 publications

References 41 publications

Continuous Microalgal Cultivation for Antioxidants Production

Continuous Microalgal Cultivation for Antioxidants Production

Outdoor scale‐up of Leptolyngbya sp.: Effect of light intensity and inoculum volume on photoinhibition and ‐oxidation

The Chemistry, Biochemistry and Pharmacology of Marine Natural Products from Leptolyngbya, a Chemically Endowed Genus of Cyanobacteria

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