Production of pigment-free pullulan by swollen cell in Aureobasidium pullulans NG which cell differentiation was affected by pH and nutrition

Li, Bingxue; Zhang, Ning; Peng, Qianqian; Yin, Tie; Guan, Feifei; Wang, Guili; Li, Ying

doi:10.1007/s00253-009-1955-2

Cited by 39 publications

(15 citation statements)

References 27 publications

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“…When pH was 6.0, the cells remained mainly of yeast‐like form after 12 h cultivation and melanin began to accumulate in the cell after 48 h (data not shown here). Early work already confirmed that pH was the key factor regulating cell morphogenesis of strain A. pullulans . Li et al proved that yeast‐like cells (YL) controlled by extracellular pH had no relationship with nutrition level, which was maintained at pH > 6.0, but was transformed into a swollen cell (SC) at pH ∼4.5.…”

Section: Resultsmentioning

confidence: 95%

“…Early work already confirmed that pH was the key factor regulating cell morphogenesis of strain A. pullulans . Li et al proved that yeast‐like cells (YL) controlled by extracellular pH had no relationship with nutrition level, which was maintained at pH > 6.0, but was transformed into a swollen cell (SC) at pH ∼4.5. SC, a stable cell type of A. pullulans , could bud, separate, or transform into meristematic structure (MS) or chlamydospore (CH), in response to nutrition level and low pH.…”

Section: Resultsmentioning

confidence: 95%

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Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by Aureobasidium pullulans ipe‐1

Cao

Zhao

et al. 2012

J of Chemical Tech & Biotech

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BACKGROUND: β‐poly(malic acid) (PMLA) can be used as a pro‐drug or for a drug‐delivery system. Effects of pH, dissolved oxygen concentration (DO) and stirring speed were investigated to improve PMLA production by A. pullulans ipe‐1. RESULTS: The strain produced a high PMLA concentration when pH and DO remained at about 6.0 and above 70%, respectively, and the yeast‐like cells were the main PMLA producers. To further promote PMLA production, the cultivation could be divided into three phases. In phase I, cell growth was accelerated by maintaining high DO (>70%) with a constant stirring speed of 800 rpm. In phase II, PMLA production was increased by controlling DO at 70% using the automatically controlled stirring speed. In phase III, PMLA production on per gram of glucose (Yp/s) was enhanced by keeping DO at 70%, and using a low stirring speed to decrease cell growth. Compared with batch cultures, a higher PMLA yield was obtained with this strategy, i.e. PMLA production and Yp/s increased by 15% and 18%, respectively. CONCLUSION: Control strategies for pH, DO and stirring speed provide a good reference for process development and optimization of PMLA production. © 2012 Society of Chemical Industry

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Section: Resultsmentioning

confidence: 95%

Section: Resultsmentioning

confidence: 95%

Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by Aureobasidium pullulans ipe‐1

Cao

Zhao

et al. 2012

J of Chemical Tech & Biotech

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“…Therefore, a relatively high concentration of CaCO 3 (∼30 g/L) was used in this study to maintain the fermentation pH around 6 as well as to stimulate PMA production. A near‐neutral pH of 6–6.5 is important for the PMA biosynthesis because a lower pH may cause the hydrolysis of PMA (at pH < 5) (Holler et al, 1992) and induce the production of extracellular polysaccharides (at pH 4.5) and chlamydospore (at pH 3) (Li et al, 2009), which may compete for limited carbon source and result in lower PMA yield.…”

Section: Resultsmentioning

confidence: 99%

Production of polymalic acid and malic acid by Aureobasidium pullulans fermentation and acid hydrolysis

Zou

Zhou

Yang

2013

Biotech & Bioengineering

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Malic acid is a dicarboxylic acid widely used in the food industry and also a potential C4 platform chemical that can be produced from biomass. However, microbial fermentation for direct malic acid production is limited by low product yield, titer, and productivity due to end-product inhibition. In this work, a novel process for malic acid production from polymalic acid (PMA) fermentation followed by acid hydrolysis was developed. First, a PMA-producing Aureobasidium pullulans strain ZX-10 was screened and isolated. This microbe produced PMA as the major fermentation product at a high-titer equivalent to 87.6 g/L of malic acid and high-productivity of 0.61 g/L h in free-cell fermentation in a stirred-tank bioreactor. Fed-batch fermentations with cells immobilized in a fibrous-bed bioreactor (FBB) achieved the highest product titer of 144.2 g/L and productivity of 0.74 g/L h. The fermentation produced PMA was purified by adsorption with IRA-900 anion-exchange resins, achieving a ∼100% purity and a high recovery rate of 84%. Pure malic acid was then produced from PMA by hydrolysis with 2 M sulfuric acid at 85°C, which followed the first-order reaction kinetics. This process provides an efficient and economical way for PMA and malic acid production, and is promising for industrial application.

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“…Besides, a low pH may also induce the formation of by-product (e.g., extracellular polysaccharides) and chlamydospore by some strains of Aureobasidium spp. [ 42 ], which is undesirable in PMA production. Another reason for this is the significant role of CaCO 3 in the biosynthesis of malic acid.…”

Section: Discussionmentioning

confidence: 99%

Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

Xia¹,

Xu²,

Liu³

et al. 2017

BMC Biotechnol

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Backgroundpoly(L-malic acid) (PMA) is a water-soluble polyester with many attractive properties in medicine and food industries, but the high cost of PMA fermentation has restricted its further application for large-scale production. To overcome this problem, PMA production from Jerusalem artichoke tubers was successfully performed. Additionally, a valuable exopolysaccharide, pullulan, was co-produced with PMA by Aureobasidum pullulans HA-4D.ResultsThe Jerusalem artichoke medium for PMA and pullulan co-production contained only 100 g/L hydrolysate sugar, 30 g/L CaCO3 and 1 g/L NaNO3. Compared with the glucose medium, the Jerusalem artichoke medium resulted in a higher PMA concentration (114.4 g/L) and a lower pullulan concentration (14.3 g/L) in a 5 L bioreactor. Meanwhile, the activity of pyruvate carboxylase and malate dehydrogenas was significantly increased, while the activity of α-phosphoglucose mutase, UDP-glucose pyrophosphorylase and glucosyltransferase was not affected. To assay the economic-feasibility, large-scale production in a 1 t fermentor was performed, yielding 117.5 g/L PMA and 15.2 g/L pullulan.ConclusionsIn this study, an economical co-production system for PMA and pullulan from Jerusalem artichoke was developed. The medium for PMA and pullulan co-production was significantly simplified when Jerusalem artichoke tubers were used. With the simplified medium, PMA production was obviously stimulated, which would be associated with the improved activity of pyruvate carboxylase and malate dehydrogenas.Electronic supplementary materialThe online version of this article (doi:10.1186/s12896-017-0340-y) contains supplementary material, which is available to authorized users.

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Production of pigment-free pullulan by swollen cell in Aureobasidium pullulans NG which cell differentiation was affected by pH and nutrition

Cited by 39 publications

References 27 publications

Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by Aureobasidium pullulans ipe‐1

Control strategy of pH, dissolved oxygen concentration and stirring speed for enhancing β‐poly (malic acid) production by Aureobasidium pullulans ipe‐1

Production of polymalic acid and malic acid by Aureobasidium pullulans fermentation and acid hydrolysis

Economic co-production of poly(malic acid) and pullulan from Jerusalem artichoke tuber by Aureobasidium pullulans HA-4D

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