Production of poly-3-hydroxybutyrate (PHB) nanoparticles using grape residues as the sole carbon source

Andler, R.; González-Arancibia, F.; Vilos, C.; Sepulveda-Verdugo, R.; Castro, R.; Mamani, M.; Valdés, C.; Arto-Paz, F.; Díaz-Barrera, A.; Martínez, I.

doi:10.1016/j.ijbiomac.2024.129649

Cited by 5 publications

(1 citation statement)

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“…Further comprehensive analysis could be undertaken to investigate the mechanical properties of the biopolymer and explore new potential applications. Examples of studies regarding to this topic include utilizing PHB nanoparticles for drug delivery [72] or exploring combinations with other materials to extend its applications, such as PHB blending with more ductile biopolymers, like poly(lactic acid), starch, cellulose and poly(caprolactone) [73][74][75][76][77], with maleic anhydride [78], or phenol mixtures from winery residues [79].…”

Section: Discussionmentioning

confidence: 99%

Bioplastic production by harnessing cyanobacteria-rich microbiomes for perpetual synthesis

Altamira-Algarra,

Lage,

Meléndez

et al. 2023

Preprint

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Departing from the conventional axenic and heterotrophic cultures, our research ventures into unexplored territory by investigating the potential of photosynthetic microbiomes for polyhydroxybutyrate (PHB) production. A cyanobacteria-rich microbiome was harnessed for PHB production in a 3 L photobioreactor under non-sterile conditions. The robust culture achieved up to 28 %dcwPHB over 108 days of alternating growth and accumulation phases. Nile Blue staining and Transmission Electron Microscope visualization successfully confirmed the presence of PHB granules within cyanobacteria cells. Analysis through proton Nuclear Magnetic Resonance further validated the extracted polymer as PHB. In addition, the overexpression of the enzyme PHA synthase throughout the accumulation phase correlated directly with the increased PHB production. Also, gene expression changes suggested that initially, glycogen served as the primary storage compound. However, with prolonged macronutrient stress, there was a shift of the carbon flux towards favoring PHB synthesis. Overall, it was demonstrated for the first time the feasibility of using a phototrophic microbiome to continuous production of PHB in a non-sterile system, contributing to advancing in the field of biopolymer production and offering valuable insights into the metabolic pathways involved.

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