Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids

Ramsay, Bruce A.; Lomaliza, K; Chavarie, C.; Dubé, Bruno-Pierre; Bataille, P.; Ramsay, J. O.

doi:10.1128/aem.56.7.2093-2098.1990

Cited by 283 publications

(122 citation statements)

References 19 publications

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“…27 However, it is evident that there was a metabolic change towards the accumulation of mcl-PHA. The experimental data support that the consumed carbon source was distributed as shown in Eqn (44) in the absence of growth. Thus, Y unbal P∕S 1 (0.15 g g -1 ) and m unbal (0.146 h -1 ) values were higher than those during the balanced growth phase, and these values adequately agree with those calculated by Durner et al 27 In addition, other studies provide evidence for this linear kinetic behavior during the batch culture storage phase.…”

Section: Model Calibration For the Mcl-pha Production On Octanoatesupporting

confidence: 74%

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Vega

Castillo

2018

J of Chemical Tech & Biotech

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BACKGROUND Poly(hydroxyalkanoates) (PHAs) are biodegradable polymers that can replace conventional plastics, but microbial production of PHAs must be optimized for commercial success. The aim of this study is to provide a simple mathematical model based on previous studies in the literature to represent the production of PHAs in chemostat and batch cultures by Pseudomonas putida GPo1 (ATCC 29347) and Cupriavidus necator (DSM 545) grown on octanoate and glucose, respectively. RESULTS Kinetic and stoichiometric equations, dependent on the specific growth rate of residual biomass (µR), were developed for carbon‐ and nitrogen‐limiting growth conditions. The parameters have biochemical significance and are independent of the biomass concentration. The results revealed that the Luedeking–Piret model is growth‐associated for both fermentations under carbon limitation. In addition, the PHA content increases with µR. Meanwhile, the PHA content is amplified under nitrogen limitation but decreases as μR increases. Also, the Luedeking–Piret model exhibits a profile that depends on the synthesized polyhydroxyalkanoate. CONCLUSION This study demonstrates that strategies for the production of PHAs require an in‐depth understanding of the process kinetics. This model gives satisfactory predictions, may be extended to fed batch cultures and may be adapted to other fermentations. © 2018 Society of Chemical Industry

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Section: Model Calibration For the Mcl-pha Production On Octanoatesupporting

confidence: 74%

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Vega

Castillo

2018

J of Chemical Tech & Biotech

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“…Cell densities and total PHA contents obtained under conditions yielding P(3HB-co-3HV) were lower than those for PHB. This is probably because of the toxic effect of propionate on growth, which needs very tight control to reach appropriate molar ratio of HV while also keeping decent polymer productivity (Ramsay et al 1990;Son and Lee 1996;Kim and Lenz 2001;Yu et al 2005). Usually, the costs of cosubstrate increase the production costs of P(3HB-co-3HV) especially if a high 3HV content is intended (Choi and Lee 2000).…”

Section: Discussionmentioning

confidence: 99%

Zobellella denitrificans strain MW1, a newly isolated bacterium suitable for poly(3-hydroxybutyrate) production from glycerol

Ibrahim¹,

Steinbüchel²

2010

Journal of Applied Microbiology

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Aims: To search for new bacteria for efficient production of polyhydroxyalkanoates (PHAs) from glycerol. Methods and Results: Samples were taken from different environments in Germany and Egypt, and bacteria capable of growing in mineral salts medium with glycerol as sole carbon source were enriched. From a wastewater sediment sample in Egypt, a Gram‐negative bacterium (strain MW1) was isolated that exhibited good growth and that accumulated considerable amounts of polyhydroxybutyrate (PHB) from glycerol and also from other carbon sources. The 16S rRNA gene sequence of this isolate exhibited 98·5% and 96·2% similarity to Zobellella denitrificans strain ZD1 and to Zobellella taiwanensis strain ZT1 respectively. The isolate was therefore affiliated as strain MW1 of Z. denitrificans. Strain MW1 grows optimally on glycerol at 41°C and pH 7·3 and accumulated PHB up to 80·4% (w/w) of cell dry weight. PHB accumulation was growth‐associated. Although it was not an absolute requirement, 20 g l−1 sodium chloride enhanced both growth (5 g cell dry weight per litre) and PHB content (87%, w/w). Zobellella denitrificans strain MW1 is also capable to accumulate the poly(3‐hydroxybutyrate‐co‐3‐hydroxyvalerate) copolymer if sodium propionate was used as cosubstrate in addition to glycerol. Conclusions: A new PHB‐accumulating strain was isolated and identified. This strain is able to utilize glycerol for growth and PHB accumulation to high content especially in the presence of NaCl that will enable the utilization of waste glycerol from biodiesel industry. Significance and Impact of the Study: This study is the first report on accumulation of PHA in a member of the new genus Zobellella. Furthermore, utilization of glycerol as the sole carbon source for fast growth and PHB biosynthesis, growth in the presence of NaCl and high PHB contents of the cells will make this newly isolated bacterium a potent candidate for industrial production of PHB from crude glycerol occurring as byproduct during biodiesel production.

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“…A number of Bacillus spp. have been reported to accumulate 9±44á5% DCW PHA (Nickerson et al 1981;Wakisaka et al 1982;Haywood et al 1989;Anderson et al 1990;Ramsay et al 1990;Kofronova et al 1994 Medium contained 20 g s l )1 sucrose, glucose and fructose, mineral salts and trace element solution in shake¯asks. Cell mass and PHB content were determined at 24 h. Control () N 2 ) 1 á2 0á14 25á8 0á3 0 á82 0á03 0á04 0á01 Peptone 3á8 0á15 75á0 0á4 5 á10 0á04 0á23 0á01 Yeast extract 3á7 0á16 78á4 0á3 5 á20 0á41 0á24 0á03 Beef extract 3á8 0á20 81á6 0á5 5 á50 0á23 0á26 0á01 Casamino acid 3á8 0á21 73á7 0á4 5 á24 0á20 0á22 0á02 (NH 4 ) 2 SO 4 3á6 0á22 69á4 0á4 4 á60 0á32 0á21…”

Section: Discussionmentioning

confidence: 99%

“…A number of Bacillus spp. have been reported to accumulate 9±44á5% dry cell weight (DCW) PHA (Nickerson et al 1981;Wakisaka et al 1982;Haywood et al 1989;Anderson et al 1990;Ramsay et al 1990;Kofronova et al 1994; . This paper describes the in¯uence of nutritional and environmental conditions on the growth of and PHB accumulation by a new strain of Bacillus mycoides RLJ B-017.…”

Section: Introductionmentioning

confidence: 99%

The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017

2002

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Aims: To optimize the nutritional and environmental conditions for growth of and poly±b-hydroxybutyrate (PHB) accumulation in Bacillus mycoides RLJ B-017. Methods and Results: An isolate, identi®ed as B. mycoides, was grown on different sources of carbon and nitrogen. Among these, sucrose, beef extract and di-ammonium sulphate were found to be the most suitable for growth and PHB accumulation. The overall maximum value of PHB (%) in cells, PHB yield (Y p/s ) and productivities (Q p and q p ) were 69á4 0á4% dry cell weight (DCW), 0á21 g p g S ±1 , 0á104 0á012 g p l ±1 h ±1 and 0á03 g p g x ±1 h ±1, respectively when grown in a medium containing 20 g s l ±1 sucrose, supplemented with di-ammonium sulphate. The addition of beef extract increased the value of PHB (%) in cells, PHB yield and productivities by 17á58 0á3, 23á8, 19á23 0á3 and 13á8 0á2%, respectively. The overall maximum values of PHB (% DCW), PHB yield and productivities were obtained at pH 7á0 0á1, temperature 30 0á5°C, agitation 650 rev min ±1 and oxygen transfer rate 3á8 mmol O 2 l ±1 h ±1 . Conclusions: Sucrose, glucose and fructose were found to be more suitable for cell growth and PHB accumulation, but sucrose was less expensive than glucose. Among the nitrogen sources, beef extract and di-ammonium sulphate promoted PHB synthesis. The accumulation of PHB was observed to be growth associated. Signi®cance and Impact of the Study: Gram-positive bacteria have not been reported to accumulate large amounts of polyhydroxyalkanoate and hence have not been considered as potent candidates for industrial production. A number of Bacillus spp. have been reported to accumulate 9±44á5% DCW PHB. By comparison, Bacillus RLJ B-017 contained 69á4 0á4% DCW PHB. Therefore, this strain has been considered as a potent organism for industrial interest. A relatively high yield of PHB was obtained in this wild strain and PHB synthesis was independent of nutrient limitation. The conditions for the higher PHB yield and productivity will be optimized in the next phase using fed-batch culture.

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Production of poly-(beta-hydroxybutyric-co-beta-hydroxyvaleric) acids

Cited by 283 publications

References 19 publications

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

A simple mathematical model capable of describing the microbial production of poly(hydroxyalkanoates) under carbon‐ and nitrogen‐limiting growth conditions

Zobellella denitrificans strain MW1, a newly isolated bacterium suitable for poly(3-hydroxybutyrate) production from glycerol

The influence of nutritional and environmental conditions on the accumulation of poly-beta-hydroxybutyrate in Bacillus mycoides RLJ B-017

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