Production of polyhydroxyalkanoate by mixed cultivation of Brevundimonas diminuta R79 and Pseudomonas balearica R90

Liang, Boya; Zhang, Xiujun; Wang, Fang; Miao, Changfeng; Ji, Yan; Huang, Zida; Gu, Pengfei; Liu, Xiaoli; Fan, Xiangyu; Li, Qiang

doi:10.1016/j.ijbiomac.2023.123667

Cited by 6 publications

(1 citation statement)

References 53 publications

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“…Notably, RNA-seq significantly contributed to revealing the importance of the ICDH gene. Liang et al [ 25 ]. used RNA-Seq technology to analyze the gene expression of two strains, Pseudomonas balearica R90 and Brevundimonas diminuta R79, during cocultivation.…”

Section: Introductionmentioning

confidence: 99%

Enhancement of vitamin B6 production driven by omics analysis combined with fermentation optimization

Tian,

Liu,

et al. 2024

Microb Cell Fact

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Background Microbial engineering aims to enhance the ability of bacteria to produce valuable products, including vitamin B6 for various applications. Numerous microorganisms naturally produce vitamin B6, yet the metabolic pathways involved are rigorously controlled. This regulation by the accumulation of vitamin B6 poses a challenge in constructing an efficient cell factory. Results In this study, we conducted transcriptome and metabolome analyses to investigate the effects of the accumulation of pyridoxine, which is the major commercial form of vitamin B6, on cellular processes in Escherichia coli. Our omics analysis revealed associations between pyridoxine and amino acids, as well as the tricarboxylic acid (TCA) cycle. Based on these findings, we identified potential targets for fermentation optimization, including succinate, amino acids, and the carbon-to-nitrogen (C/N) ratio. Through targeted modifications, we achieved pyridoxine titers of approximately 514 mg/L in shake flasks and 1.95 g/L in fed-batch fermentation. Conclusion Our results provide insights into pyridoxine biosynthesis within the cellular metabolic network for the first time. Our comprehensive analysis revealed that the fermentation process resulted in a remarkable final yield of 1.95 g/L pyridoxine, the highest reported yield to date. This work lays a foundation for the green industrial production of vitamin B6 in the future.

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Section: Introductionmentioning

confidence: 99%