Production of polyhydroxyalkanoates by Herbaspirillum seropedicae grown with different sole carbon sources and on lactose when engineered to express the lacZlacY genes

Catalán, Ana Inés; Ferreira, Fernando; Gill, Paul R.; Batista, Silvia

doi:10.1016/j.enzmictec.2006.10.008

Cited by 37 publications

(27 citation statements)

References 29 publications

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“…H. seropedicae SmR1 has been already described as a PHB producer using glucose as carbon source [28], however the molecular aspects of its PHB metabolism have not been addressed. The H. seropedicae SmR1 genome sequence analysis indicated several genes likely to be involved in PHB metabolism, including, phbA, phbB and phbC encoding for 3-ketothiolase, acetoacetyl-CoA reductase and poly(3-hydroxybutyrate) synthase.…”

Section: Introductionmentioning

confidence: 99%

Identification and characterization of PhbF: A DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1

et al. 2011

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BackgroundHerbaspirillum seropedicae SmR1 is a nitrogen fixing endophyte associated with important agricultural crops. It produces polyhydroxybutyrate (PHB) which is stored intracellularly as granules. However, PHB metabolism and regulatory control is not yet well studied in this organism.ResultsIn this work we describe the characterization of the PhbF protein from H. seropedicae SmR1 which was purified and characterized after expression in E. coli. The purified PhbF protein was able to bind to eleven putative promoters of genes involved in PHB metabolism in H. seropedicae SmR1. In silico analyses indicated a probable DNA-binding sequence which was shown to be protected in DNA footprinting assays using purified PhbF. Analyses using lacZ fusions showed that PhbF can act as a repressor protein controlling the expression of PHB metabolism-related genes.ConclusionsOur results indicate that H. seropedicae SmR1 PhbF regulates expression of phb-related genes by acting as a transcriptional repressor. The knowledge of the PHB metabolism of this plant-associated bacterium may contribute to the understanding of the plant-colonizing process and the organism's resistance and survival in planta.

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Section: Introductionmentioning

confidence: 99%

Identification and characterization of PhbF: A DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1

et al. 2011

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“…PHB production from concentrated whey solution was obtained by high-density cultivation of recombinant E. coli and resulted in polymer content as high as 80% [8]. Herbaspirillum seropedicae Z69 (pHM3) grown on lactose as a sole carbon source and when engineered to express the lacZlacY genes accumulated PHB as high as that of wild-type T. thermophilus [32].…”

Section: Discussionmentioning

confidence: 99%

Production of polyhydroxyalkanoates from whey by Thermus thermophilus HB8

Pantazaki

Papaneophytou

Pritsa

et al. 2009

Process Biochemistry

104

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“…Growth associated P(3HB) accumulation was recorded in about 43.5% of the total endophytic isolates and the P(3HB) content of these positive isolates ranged from 4.72-34.36% of their cell dry weight (CDW). These findings received support from the production of P(3HB) by endophytic diazotrophs [6,7] as well as heterotrophs [30]. Out of these 23 isolates, bacterial strain HAL 03, endophytic to leaf of H. annuus L. appeared to be the most promising one showing the highest intracellular accumulation of polyester (34.36% of CDW) and was selected for detailed studies.…”

Section: Screening and Selection Of Endophytic Bacteria For P(3hb) Prmentioning

confidence: 97%

“…While, the plant rhizosphere is an interesting hidden niche for polyhydroxyalkanoate producers [3,4,5], the culturable diversity of bacteria endophytic to plants have attracted the attention in recent years for the production of PHAs. Catalan et al [6] have reported the accumulation of P(3HB) in the diazotrophic endophyte Herbaspirillum seropedicae Z69 colonizing a variety of higher plants. The bacterium accumulated 36% of its biomass as P(3HB) when grown in glucose containing medium.…”

Section: Introductionmentioning

confidence: 99%

Influence of growth conditions on production of poly(3-hydroxybutyrate) by Bacillus cereus HAL 03 endophytic to Helianthus annuus L.

2016

J App Biol Biotech

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Systematic screening of culturable endophytic bacteria for production of poly(3-hydroxybutyric acid) [P(3HB)] has resulted in the isolation of a potent strain identified as Bacillus cereus HAL 03 (GenBank Accession No. KR869088) from leaf tissue of Helianthus annuus L. Production of P(3HB) by this strain was influenced significantly by the quality as well as quantity of suitable carbon and nitrogen sources in the growth medium. At 2% (w/v) sucrose, the accumulated P(3HB) reached to a level of 50.46 % of its cell dry weight (CDW), whereas yeast extract (0.2%) as nitrogen source enhanced P(3HB) production and accounted for 53.19% of CDW. Moreover, the non-conventional carbon sources such as molasses further increased the production of the polyester up to 54.05% of its CDW. Finally, the identity of this intracellularly accumulated polyester was confirmed as a homopolymer of 3(hydroxybutyric acid) by the Fourier-transform infrared and proton nuclear magnetic resonance spectroscopic analysis. This study appears to indicate the first ever report of P(3HB) production by any strain of B. cereus endophytic to H. annuus L.

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Production of polyhydroxyalkanoates by Herbaspirillum seropedicae grown with different sole carbon sources and on lactose when engineered to express the lacZlacY genes

Cited by 37 publications

References 29 publications

Identification and characterization of PhbF: A DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1

Identification and characterization of PhbF: A DNA binding protein with regulatory role in the PHB metabolism of Herbaspirillum seropedicae SmR1

Production of polyhydroxyalkanoates from whey by Thermus thermophilus HB8

Influence of growth conditions on production of poly(3-hydroxybutyrate) by Bacillus cereus HAL 03 endophytic to Helianthus annuus L.

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