Production of polyhydroxyalkanoates from methanol by a new methylotrophic bacterium Methylobacterium sp. GW2

Yezza, Abdessalem; Fournier, Diane; Halasz, Annamaria; Hawari, Jalal

doi:10.1007/s00253-006-0458-7

Cited by 73 publications

(43 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…This indicated that methanol is the principal precursor for PHA synthesis by strain Azu-IN1 as previously reported [33][34]. Besides, the recovery yield is superior to that obtained to Yezza et al, [35] who reported that, after 66 h, a copolymer content of 30 % (w/w) was achieved when 1 % (v/v) methanol solution supplemented with 0.1 % (v/v) valeric acid used as substrate. In addition, the yield (Y p/s ) of 0.228 g/g obtained in this study is about 84.5 % of the maximum theoretical yield based on glycerol (0.47 g/g) via acetyl-CoA pathway [22].…”

Section: Discussionsupporting

confidence: 54%

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

2017

J App Biol Biotech

View full text Add to dashboard Cite

Polyhydroxyalkalonates (PHAs) are biodegradable thermoplastics that are receiving immense attention as an alternative to petroleum derived plastics. The present study aimed to produce PHA using cheap and ecofriendly biodiesel waste glycerol as a substrate. Several PHA-producing bacterial isolates were isolated from different environmental samples and their efficacy for PHA production was assessed. Isolate Azu-IN1 showed the highest PHA production and was identified as Zobellella taiwanensis Azu-IN1 using 16S rRNA gene sequence and biochemical characterization. Factors affecting PHA production were optimized in batch fermentations. Supplementation of ammonium chloride as nitrogen source, methanol as an auxiliary carbon source, and agitation rate are the main limiting factors affecting PHA production. Maximum PHA production of 2.65 g/L at recovery yield of 50.3 (%), w/w) was achieved after 36 h in batch fermentation using optimized medium. Enhanced PHA production of 3.73 g/L with recovery yield of 61.7 % (w/w) was obtained in fed batch fermentation. The characteristics of extracted PHA were analyzed using Fourier Transform Infrared Spectroscopy (FTIR) 1 H and 13 C Nuclear Magnetic Resonance (NMR) spectroscopy. This is the first report on accumulation of PHA by Zobellella taiwanensis using glycerol as the sole carbon source.

show abstract

Section: Discussionsupporting

confidence: 54%

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

2017

J App Biol Biotech

View full text Add to dashboard Cite

show abstract

“…Whereas Yamane et al (1996) produced PHB with a M n of 172×10 3 (M w /M n 1.84) in a high-cell-density fedbatch culture of A. latus using a sucrose-based medium. Discrepancy between M n obtained in our study and those of Yamane et al (1996) could be attributed to the differences in growth conditions, biopolymer extraction, purification techniques, or the purity of samples (Rehm 2003;Yezza et al 2006). The molecular weight of polymers is believed to be one of the most important factors affecting their physicochemical and mechanical properties and thus their applications.…”

Section: Discussionmentioning

confidence: 93%

Production of poly-β-hydroxybutyrate (PHB) by Alcaligenes latus from maple sap

Yezza

Halasz

Levadoux

et al. 2007

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

/npsi/ctrl?action=rtdoc&an=3539342&lang=en http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/ctrl?action=rtdoc&an=3539342&lang=frAccess and use of this website and the material on it are subject to the Terms and Conditions set forth at http://nparc.cisti-icist.nrc-cnrc.gc.ca/npsi/jsp/nparc_cp.jsp?lang=en NRC Publications Archive Archives des publications du CNRCThis publication could be one of several versions: author's original, accepted manuscript or the publisher's version. / La version de cette publication peut être l'une des suivantes : la version prépublication de l'auteur, la version acceptée du manuscrit ou la version de l'éditeur.Applied Microbiology and Biotechnology, 77, 2, Production of Poly-β-Hydroxybutyrate ( Abstract Maple sap, an abundant natural product especially in Canada, is rich in sucrose and thus may represent an ideal renewable feedstock for the production of a wide variety of value-added products. In the present study, maple sap or sucrose was employed as a carbon source to Alcaligenes latus for the production of poly-β-hydroxybutyrate (PHB). In shake flasks, the biomass obtained from both the sap and sucrose were 4.4±0.5 and 2.9±0.3 g/L, and the PHB contents were 77.6±1.5 and 74.1±2.0%, respectively. Subsequent batch fermentation (10 L sap) resulted in the formation of 4.2±0.3 g/L biomass and a PHB content of 77.0±2.6%. The number average molecular weights of the PHB produced by A. latus from maple sap and pure sucrose media were 300±66×10 3 and 313±104×10 3 g/mol, respectively. Near-infrared, 1 H magnetic resonance imaging (MRI), and 13 C-MRI spectra of the microbially produced PHB completely matched those obtained with a reference material of poly[(R)-3-hydroxybutyric acid]. The polymer was found to be optically active with [α] 25 D equaled to −7.87 in chloroform. The melting point (177.0°C) and enthalpy of fusion (77.2 J/g) of the polymer were also in line with those reported, i.e., 177°C and 81 J/g, respectively.

show abstract

“…The HV source might be poly 3-hydroxybutyrate-co-3-hydroxyvalerate (PHBV) known to be available in soil film and degraded by microbial activity especially by some fungal strains (Sang et al 2002). The production of PHBV in soil is associated with some bacterial strains such as Methylobacterium organophilum under nitrogen limitation (Yezza et al 2006). In our study, the increase of hydroxyvalerate was reflected on foxtail shoots uptake in the groups treated with mycorrhiza only (EC and EN) and showed a positive correlation with foxtail height and fresh and dry biomass.…”

Section: Discussionmentioning

confidence: 99%

Metabolomics, biomass and lignocellulosic total sugars analysis in foxtail millet (Setaria italica) inoculated with different combinations of plant growth promoting bacteria and mycorrhiza

Dhawi¹,

Datta²,

Ramakrishna³

2018

Commun. Plant Sci.

View full text Add to dashboard Cite

Foxtail millet (Setaria italica) is the second most widely produced millet with potential as a biofuel source. Employment of plant growth promoting bacteria (PGPB) and mycorrhiza could serve as environment-friendly alternatives for the use of excessive NPK fertilizers and producing biofuel. The highest increase of biomass was associated with endomycorrhiza combined with PGPB in comparison to control. Nuclear magnetic resonance (NMR) analysis detected 28 metabolites in foxtail shoot with most of them upregulated in ecto/endomycorrhiza group and combined with PGPB. The upregulation of metabolites associated with synthesis of amino acids correlated positively with biomass. The inoculation with both PGPB and endomycorrhiza gave the best results with reference to total sugar yield. Our study indicates that PGPB and endomycorrhiza combination is well suited for enhancing biomass and boosting sugar yield, which are useful attributes for utilizing foxtail millet as a biofuel source. Highlighted Conclusions1. Effect of PGPB and mycorrhiza on foxtail millet biomass, metabolites and total sugars was studied. 2. Biomass and total sugars increased by combined PGPB and endomycorrhiza treatment. 3. Higher biomass correlated positively with metabolites associated with amino acid biosynthesis.Foxtail millet (Setaria italica) is a diploid C4 grass with a relatively small genome (~515 Mb) (Li and Brutnell 2011). Foxtail millet is an annual grass that is widely used for both food and livestock feed and grown in arid and semi-arid regions of the world (Pandey et al. 2017). Being a close relative of an important biofuel crop switchgrass, it is also a potential biofuel source. The early maturity of foxtail in 60-70 days makes it a good model for experimental studies. Several recent studies have explored the molecular responses of foxtail millet under abiotic stress. Colonization of arbuscular mycorrhizal (AM) fungus Glomus mosseae and its effect on phosphate transport was reported previously, where a significant increase in the expression of plant phosphate transporters belonging to PHT1 family in low phosphate environment was reported in the presence of AM fungi (Ceasar et al. 2014). PHT1 transporter gene family of foxtail millet consists of 12 members with varying levels of expression depending on the availability of phosphate and AM colonization, which helps in enhancing phosphate availability.The plant growth promoting bacteria used in this study was isolated from Torch Lake sediments rich in copper deposits and was shown to enhance maize growth and metal uptake (Li and Ramakrishna 2011). Previously, we reported higher biomass and element uptake in sorghum and maize and upregulation of stress tolerance associated proteins in sorghum as a result of root colonization of PGPB and AM fungi (Dhawi et al. 2015(Dhawi et al. , 2016

show abstract

Production of polyhydroxyalkanoates from methanol by a new methylotrophic bacterium Methylobacterium sp. GW2

Cited by 73 publications

References 22 publications

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

Fermentative Production of Polyhydroxyalkanoates (PHAs) from Glycerol by Zobellella taiwanensis Azu-IN1

Production of poly-β-hydroxybutyrate (PHB) by Alcaligenes latus from maple sap

Metabolomics, biomass and lignocellulosic total sugars analysis in foxtail millet (Setaria italica) inoculated with different combinations of plant growth promoting bacteria and mycorrhiza

Contact Info

Product

Resources

About